E.coli DNA Replication Experiment
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Questions and Answers

What is the main enzyme involved in the process of DNA replication in E.coli?

DNA-dependent DNA polymerase

How long does it take for E.coli, with 4.6 x 10^6 base pairs, to complete the process of replication?

18 minutes

What is the average rate of polymerization required by E.coli during DNA replication?

2000 base pairs per second

What is the consequence of a mistake during DNA replication?

<p>Mutations</p> Signup and view all the answers

What serves dual purposes during the process of DNA replication?

<p>Deoxyribonucleoside triphosphates</p> Signup and view all the answers

What provides energy for polymerisation reactions in DNA replication?

<p>Deoxynucleoside triphosphates</p> Signup and view all the answers

What catalyzes polymerisation in only one direction during DNA replication?

<p>DNA-dependent DNA polymerases</p> Signup and view all the answers

What enzyme joins the discontinuously synthesized fragments during DNA replication?

<p>DNA ligase</p> Signup and view all the answers

What is the region in E.coli DNA where replication originates called?

<p>Origin of replication</p> Signup and view all the answers

Why does replication occur within a small opening of the DNA helix for long DNA molecules?

<p>Due to very high energy requirement</p> Signup and view all the answers

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