10 Questions
What is the main enzyme involved in the process of DNA replication in E.coli?
DNA-dependent DNA polymerase
How long does it take for E.coli, with 4.6 x 10^6 base pairs, to complete the process of replication?
18 minutes
What is the average rate of polymerization required by E.coli during DNA replication?
2000 base pairs per second
What is the consequence of a mistake during DNA replication?
Mutations
What serves dual purposes during the process of DNA replication?
Deoxyribonucleoside triphosphates
What provides energy for polymerisation reactions in DNA replication?
Deoxynucleoside triphosphates
What catalyzes polymerisation in only one direction during DNA replication?
DNA-dependent DNA polymerases
What enzyme joins the discontinuously synthesized fragments during DNA replication?
DNA ligase
What is the region in E.coli DNA where replication originates called?
Origin of replication
Why does replication occur within a small opening of the DNA helix for long DNA molecules?
Due to very high energy requirement
Explore the proportions of light and hybrid densities of DNA molecules after E.coli is allowed to grow for 80 minutes. Learn about the semiconservative replication of DNA in chromosomes based on experiments with Vicia faba (faba beans) by Taylor and colleagues in 1958.
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