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Questions and Answers

What is the primary function of oxygen in aerobic respiration?

  • To act as the final electron acceptor (correct)
  • To produce glucose
  • To release carbon dioxide
  • To generate ATP directly

What is produced during the light reactions of photosynthesis?

  • Carbon dioxide
  • Oxygen and ATP (correct)
  • Glucose
  • NADPH only

What role does H2O play in the process of photosynthesis?

  • It is a final product
  • It absorbs carbon dioxide
  • It acts as a catalyst for glucose production
  • It donates protons and electrons during the light reaction (correct)

During the Calvin cycle, which enzyme plays a key role in carbon fixation?

<p>Rubisco (B)</p>
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What is the direction of electron flow starting from the H2O molecule in photosynthesis?

<p>From H2O to NADP+ (D)</p>
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Which type of molecules can pass through a semi-permeable membrane most easily?

<p>Small non-polar molecules (D)</p>
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What happens to a plant cell when placed in a hypotonic solution?

<p>The cell becomes turgid (A)</p>
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Which of the following correctly defines the 'fluid mosaic' model?

<p>It illustrates the membrane as a dynamic structure with movable components. (D)</p>
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In terms of energy, how is catabolism classified?

<p>Exergonic (D)</p>
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What is the primary impact of competitive inhibition on enzyme activity?

<p>It increases the concentration of substrate required to reach maximum reaction rate. (B)</p>
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During aerobic respiration, at which stage is Acetyl CoA produced?

<p>Transition Reaction (C)</p>
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What role does NAD+ play in cellular respiration?

<p>It serves as an electron carrier and is reduced to NADH. (D)</p>
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Which of the following describes osmotic lysis?

<p>It is the process where an animal cell bursts due to excess water intake. (C)</p>
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Study Notes

Eukaryotic Cell Structure

  • Animal and Plant Cells: Share many common structures, including the nucleus, cytoplasm, ribosomes, endoplasmic reticulum, Golgi apparatus, mitochondria, lysosomes, and peroxisomes.
  • Plant Cells: Have additional structures: cell wall, chloroplasts, and vacuoles.
  • Nucleus: Contains DNA, the cell's genetic material, and controls cellular activities.
  • Cytoplasm: Gel-like substance that fills the cell, providing a medium for organelle function.
  • Ribosomes: Sites of protein synthesis, found both free in the cytoplasm and attached to the endoplasmic reticulum.
  • Endoplasmic Reticulum (ER): Network of interconnected membranes involved in protein synthesis (rough ER) and lipid metabolism (smooth ER).
  • Golgi Apparatus: Modifies, sorts, and packages proteins and lipids for secretion or delivery to other organelles.
  • Mitochondria: Powerhouses of the cell, responsible for cellular respiration and ATP production.
  • Lysosomes: Contain enzymes that break down cellular waste and debris.
  • Peroxisomes: Involved in detoxification and lipid metabolism.
  • Cell Wall: Provides structural support and protection to plant cells.
  • Chloroplasts: Sites of photosynthesis, converting sunlight into chemical energy.
  • Vacuoles: Large storage compartments in plant cells, containing water and other substances.

Fluid Mosaic Model

  • Fluid: The phospholipid bilayer is constantly moving and rearranging, allowing for flexibility.
  • Mosaic: The membrane is composed of diverse molecules, including phospholipids, proteins, carbohydrates, and cholesterol, arranged in a mosaic pattern.

Semi-Permeable Membrane

  • Permeable: Allows certain molecules to pass through freely.
  • Small: Small molecules can pass through more easily than large molecules.
  • Non-polar: Non-polar molecules pass through more easily than polar molecules.

Solutions and Osmosis

  • Isotonic: A solution with equal solute concentration to the cell, resulting in no net water movement.

  • Hypertonic: A solution with higher solute concentration than the cell, causing water to move out of the cell.

  • Hypotonic: A solution with lower solute concentration than the cell, causing water to move into the cell.

  • Hypotonic Solution:

    • Plant Cell: Will swell due to water intake but will not burst due to the cell wall.
    • Animal Cell: May swell and burst due to lack of cell wall.
  • Hypertonic Solution:

    • Plant Cell: Will lose water and shrink, potentially leading to plasmolysis
    • Animal Cell: Will shrink and shrivel.
  • Osmotic Lysis: Bursting of a cell due to excessive water intake in a hypotonic solution. This primarily affects animal cells due to the lack of a rigid cell wall.

  • Plasmolysis: The shrinking of the cytoplasm away from the cell wall in a hypertonic solution, a process that primarily affects plant cells. This does not occur in animal cells.

Energy and Chemical Reactions

  • Endogonic Reactions: Require energy input to proceed, have a positive ΔG (change in Gibbs free energy).
  • Exergonic Reactions: Release energy, have a negative ΔG.
  • Catabolism: The breakdown of complex molecules into simpler ones, releasing energy. It is an exergonic process.
  • Anabolism: The synthesis of complex molecules from simpler ones, requiring energy input. It is an endogonic process.

Enzyme Regulation

  • Temperature (Tm): Enzymes have an optimal temperature range, activity increases with temperature up to a point, after which it denatures.
  • pH: Enzymes have an optimal pH range, deviating from this range can decrease activity or denature the enzyme.
  • Substrate Concentration: Activity increases with substrate concentration until it reaches a saturation point where all active sites are occupied.

Competitive and Non-competitive Inhibition

  • Competitive Inhibition: Inhibitor binds to the active site, competing with the substrate. Increasing substrate concentration can overcome this inhibition.
  • Non-competitive Inhibition: Inhibitor binds to an allosteric site on the enzyme, changing its shape and preventing substrate binding. Increasing substrate concentration does not overcome this inhibition.

Redox Reactions

  • Oxidation: Loss of electrons, gain of oxygen, increase in oxidation state.

  • Reduction: Gain of electrons, loss of oxygen, decrease in oxidation state.

  • C6H12O6 + O2 → CO2 + H2O + ATP

    • Oxidized: Glucose (C6H12O6)
    • Reduced: Oxygen (O2)
    • Reducing Agent: Glucose (C6H12O6)
    • Oxidizing Agent: Oxygen (O2)

Aerobic Respiration

  • Major Steps:

    • Glycolysis: Glucose is broken down into pyruvate, producing 2 ATP and 2 NADH.
    • Preparation Step: Pyruvate is converted to acetyl-CoA, producing 1 NADH.
    • Krebs Cycle (Citric Acid Cycle): Acetyl-CoA is oxidized, producing 3 NADH, 1 FADH2, 1 ATP, and 2 CO2.
    • Electron Transport Chain: Electrons from NADH and FADH2 are passed along the electron transport chain to generate ATP through oxidative phosphorylation.
  • Products: NADH, FADH2, ATP, CO2, H2O

  • NAD+ and FAD: Electron carriers, accepting electrons during oxidation and donating them during reduction.

  • Lack of FAD: Will impact the Krebs Cycle, as FADH2 is a critical electron carrier.

  • Electron Flow: From glucose to NADH to the electron transport chain to oxygen.

  • CO2 Production: Occurs during the preparation step and Krebs cycle.

  • Acetyl-CoA Production: Occurs during the preparation step.

  • ATP Production:

    • Substrate-level phosphorylation: 2 ATP produced during glycolysis and 2 ATP produced during the Krebs Cycle.
    • Oxidative phosphorylation: 28 ATP produced through the electron transport chain.
  • Proton Force: The potential energy stored across the inner mitochondrial membrane due to the proton gradient.

  • Electron Transfer and Chemiosmosis: Occur in the inner mitochondrial membrane.

  • Oxygen Function: Final electron acceptor in the electron transport chain, accepting electrons and protons to form water.

Fermentation

  • Final Acceptor: Organic molecules like pyruvate or acetaldehyde.
  • ATP: 2 ATP are produced through substrate-level phosphorylation.

Photosynthesis

  • Oxygen Production: During the light reaction, water molecules are split, releasing oxygen. The oxygen atoms are derived from the water molecule.
  • Chlorophyll II Absorption: Leads to excitation of electrons in the chlorophyll and the initiation of electron transport.
  • Final Electron Acceptor for Photosystem I: NADP+ is the final electron acceptor, forming NADPH.
  • Pigment Molecule Function: Absorb light energy and transfer it to the reaction centers where photochemical reactions occur.
  • Linear Electron Flow Products: ATP, NADPH, and oxygen.
  • Calvin Cycle Function: To use ATP and NADPH from the light reaction to fix carbon dioxide and generate glucose.
  • Electron Flow Direction: From water molecules to photosystem II to photosystem I to NADP+.

Cellular Location of Events

  • Photosynthesis:

    • Oxygen production: Chloroplasts.
    • Activated chlorophyll donates an electron: Chloroplasts.
    • Rubisco catalyzes carbon fixation: Chloroplasts.
  • Cellular Respiration:

    • ATP production in animal cells: Mitochondria.
    • Electron transportation in animal cells: Inner mitochondrial membrane.

Order of Photosynthesis Events

  • Water split to produce oxygen
  • Chlorophyll a excited and transfer electrons
  • ATP production
  • NADPH production
  • CO2 fixation
  • Glucose production

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