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Questions and Answers
What is the primary function of oxygen in aerobic respiration?
What is the primary function of oxygen in aerobic respiration?
What is produced during the light reactions of photosynthesis?
What is produced during the light reactions of photosynthesis?
What role does H2O play in the process of photosynthesis?
What role does H2O play in the process of photosynthesis?
During the Calvin cycle, which enzyme plays a key role in carbon fixation?
During the Calvin cycle, which enzyme plays a key role in carbon fixation?
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What is the direction of electron flow starting from the H2O molecule in photosynthesis?
What is the direction of electron flow starting from the H2O molecule in photosynthesis?
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Which type of molecules can pass through a semi-permeable membrane most easily?
Which type of molecules can pass through a semi-permeable membrane most easily?
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What happens to a plant cell when placed in a hypotonic solution?
What happens to a plant cell when placed in a hypotonic solution?
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Which of the following correctly defines the 'fluid mosaic' model?
Which of the following correctly defines the 'fluid mosaic' model?
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In terms of energy, how is catabolism classified?
In terms of energy, how is catabolism classified?
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What is the primary impact of competitive inhibition on enzyme activity?
What is the primary impact of competitive inhibition on enzyme activity?
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During aerobic respiration, at which stage is Acetyl CoA produced?
During aerobic respiration, at which stage is Acetyl CoA produced?
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What role does NAD+ play in cellular respiration?
What role does NAD+ play in cellular respiration?
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Which of the following describes osmotic lysis?
Which of the following describes osmotic lysis?
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Study Notes
Eukaryotic Cell Structure
- Animal and Plant Cells: Share many common structures, including the nucleus, cytoplasm, ribosomes, endoplasmic reticulum, Golgi apparatus, mitochondria, lysosomes, and peroxisomes.
- Plant Cells: Have additional structures: cell wall, chloroplasts, and vacuoles.
- Nucleus: Contains DNA, the cell's genetic material, and controls cellular activities.
- Cytoplasm: Gel-like substance that fills the cell, providing a medium for organelle function.
- Ribosomes: Sites of protein synthesis, found both free in the cytoplasm and attached to the endoplasmic reticulum.
- Endoplasmic Reticulum (ER): Network of interconnected membranes involved in protein synthesis (rough ER) and lipid metabolism (smooth ER).
- Golgi Apparatus: Modifies, sorts, and packages proteins and lipids for secretion or delivery to other organelles.
- Mitochondria: Powerhouses of the cell, responsible for cellular respiration and ATP production.
- Lysosomes: Contain enzymes that break down cellular waste and debris.
- Peroxisomes: Involved in detoxification and lipid metabolism.
- Cell Wall: Provides structural support and protection to plant cells.
- Chloroplasts: Sites of photosynthesis, converting sunlight into chemical energy.
- Vacuoles: Large storage compartments in plant cells, containing water and other substances.
Fluid Mosaic Model
- Fluid: The phospholipid bilayer is constantly moving and rearranging, allowing for flexibility.
- Mosaic: The membrane is composed of diverse molecules, including phospholipids, proteins, carbohydrates, and cholesterol, arranged in a mosaic pattern.
Semi-Permeable Membrane
- Permeable: Allows certain molecules to pass through freely.
- Small: Small molecules can pass through more easily than large molecules.
- Non-polar: Non-polar molecules pass through more easily than polar molecules.
Solutions and Osmosis
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Isotonic: A solution with equal solute concentration to the cell, resulting in no net water movement.
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Hypertonic: A solution with higher solute concentration than the cell, causing water to move out of the cell.
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Hypotonic: A solution with lower solute concentration than the cell, causing water to move into the cell.
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Hypotonic Solution:
- Plant Cell: Will swell due to water intake but will not burst due to the cell wall.
- Animal Cell: May swell and burst due to lack of cell wall.
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Hypertonic Solution:
- Plant Cell: Will lose water and shrink, potentially leading to plasmolysis
- Animal Cell: Will shrink and shrivel.
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Osmotic Lysis: Bursting of a cell due to excessive water intake in a hypotonic solution. This primarily affects animal cells due to the lack of a rigid cell wall.
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Plasmolysis: The shrinking of the cytoplasm away from the cell wall in a hypertonic solution, a process that primarily affects plant cells. This does not occur in animal cells.
Energy and Chemical Reactions
- Endogonic Reactions: Require energy input to proceed, have a positive ΔG (change in Gibbs free energy).
- Exergonic Reactions: Release energy, have a negative ΔG.
- Catabolism: The breakdown of complex molecules into simpler ones, releasing energy. It is an exergonic process.
- Anabolism: The synthesis of complex molecules from simpler ones, requiring energy input. It is an endogonic process.
Enzyme Regulation
- Temperature (Tm): Enzymes have an optimal temperature range, activity increases with temperature up to a point, after which it denatures.
- pH: Enzymes have an optimal pH range, deviating from this range can decrease activity or denature the enzyme.
- Substrate Concentration: Activity increases with substrate concentration until it reaches a saturation point where all active sites are occupied.
Competitive and Non-competitive Inhibition
- Competitive Inhibition: Inhibitor binds to the active site, competing with the substrate. Increasing substrate concentration can overcome this inhibition.
- Non-competitive Inhibition: Inhibitor binds to an allosteric site on the enzyme, changing its shape and preventing substrate binding. Increasing substrate concentration does not overcome this inhibition.
Redox Reactions
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Oxidation: Loss of electrons, gain of oxygen, increase in oxidation state.
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Reduction: Gain of electrons, loss of oxygen, decrease in oxidation state.
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C6H12O6 + O2 → CO2 + H2O + ATP
- Oxidized: Glucose (C6H12O6)
- Reduced: Oxygen (O2)
- Reducing Agent: Glucose (C6H12O6)
- Oxidizing Agent: Oxygen (O2)
Aerobic Respiration
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Major Steps:
- Glycolysis: Glucose is broken down into pyruvate, producing 2 ATP and 2 NADH.
- Preparation Step: Pyruvate is converted to acetyl-CoA, producing 1 NADH.
- Krebs Cycle (Citric Acid Cycle): Acetyl-CoA is oxidized, producing 3 NADH, 1 FADH2, 1 ATP, and 2 CO2.
- Electron Transport Chain: Electrons from NADH and FADH2 are passed along the electron transport chain to generate ATP through oxidative phosphorylation.
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Products: NADH, FADH2, ATP, CO2, H2O
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NAD+ and FAD: Electron carriers, accepting electrons during oxidation and donating them during reduction.
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Lack of FAD: Will impact the Krebs Cycle, as FADH2 is a critical electron carrier.
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Electron Flow: From glucose to NADH to the electron transport chain to oxygen.
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CO2 Production: Occurs during the preparation step and Krebs cycle.
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Acetyl-CoA Production: Occurs during the preparation step.
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ATP Production:
- Substrate-level phosphorylation: 2 ATP produced during glycolysis and 2 ATP produced during the Krebs Cycle.
- Oxidative phosphorylation: 28 ATP produced through the electron transport chain.
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Proton Force: The potential energy stored across the inner mitochondrial membrane due to the proton gradient.
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Electron Transfer and Chemiosmosis: Occur in the inner mitochondrial membrane.
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Oxygen Function: Final electron acceptor in the electron transport chain, accepting electrons and protons to form water.
Fermentation
- Final Acceptor: Organic molecules like pyruvate or acetaldehyde.
- ATP: 2 ATP are produced through substrate-level phosphorylation.
Photosynthesis
- Oxygen Production: During the light reaction, water molecules are split, releasing oxygen. The oxygen atoms are derived from the water molecule.
- Chlorophyll II Absorption: Leads to excitation of electrons in the chlorophyll and the initiation of electron transport.
- Final Electron Acceptor for Photosystem I: NADP+ is the final electron acceptor, forming NADPH.
- Pigment Molecule Function: Absorb light energy and transfer it to the reaction centers where photochemical reactions occur.
- Linear Electron Flow Products: ATP, NADPH, and oxygen.
- Calvin Cycle Function: To use ATP and NADPH from the light reaction to fix carbon dioxide and generate glucose.
- Electron Flow Direction: From water molecules to photosystem II to photosystem I to NADP+.
Cellular Location of Events
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Photosynthesis:
- Oxygen production: Chloroplasts.
- Activated chlorophyll donates an electron: Chloroplasts.
- Rubisco catalyzes carbon fixation: Chloroplasts.
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Cellular Respiration:
- ATP production in animal cells: Mitochondria.
- Electron transportation in animal cells: Inner mitochondrial membrane.
Order of Photosynthesis Events
- Water split to produce oxygen
- Chlorophyll a excited and transfer electrons
- ATP production
- NADPH production
- CO2 fixation
- Glucose production
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