Dolichol Phosphate and Protein Glycosylation Quiz
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Questions and Answers

What is the difference between N-linked and O-linked glycosylation?

  • N-linked glycosylation adds sugars sequentially to the polypeptide chain, while O-linked glycosylation adds sugars one at a time.
  • N-linked glycosylation adds sugars one at a time to the polypeptide chain, while O-linked glycosylation adds a whole bunch of sugars. (correct)
  • N-linked glycosylation adds a whole bunch of sugars to the polypeptide chain, while O-linked glycosylation adds sugars sequentially.
  • N-linked glycosylation and O-linked glycosylation are the same process.
  • Which molecule is responsible for transferring sugars from the cytosol to the ER lumen?

  • Glucose
  • Mannose
  • Dolichol phosphate (correct)
  • N-acetylglucosamine
  • What is the role of the protein that can flip the dolichol phosphate?

  • To transfer sugars from the cytosol to the ER lumen (correct)
  • To break down the dolichol phosphate
  • To transfer sugars from the ER lumen to the cytosol
  • To attach the dolichol phosphate to the polypeptide chain
  • Study Notes

    Glycosylation Types

    • N-linked glycosylation: occurs in the endoplasmic reticulum (ER) and involves the attachment of a carbohydrate molecule to an asparagine residue in a protein
    • O-linked glycosylation: occurs in the Golgi apparatus and involves the attachment of a carbohydrate molecule to a serine or threonine residue in a protein

    Glycosylation Process

    • The molecule responsible for transferring sugars from the cytosol to the ER lumen is oligosaccharyltransferase (OST)
    • Oligosaccharyltransferase (OST) transfers a pre-assembled oligosaccharide from dolichol phosphate to the asparagine residue of a protein

    Dolichol Phosphate Function

    • The protein that can flip dolichol phosphate is called flippase
    • Flippase is responsible for flipping dolichol phosphate from the cytosolic side to the luminal side of the ER, allowing the transfer of sugars to occur

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    Description

    Test your knowledge on dolichol phosphate and its role in protein glycosylation. Explore the process of sugar transfer from the cytosol to the ER lumen and learn about N-linked glycosylation.

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