Podcast Beta
Questions and Answers
What is the purpose of DNA cloning?
What technique uses a gel as a molecular sieve to separate nucleic acids or proteins by size, electrical charge, and other properties?
Which method is used to compare mRNA from different developmental stages?
What does Southern blotting combine with gel electrophoresis of DNA fragments?
Signup and view all the answers
What is the purpose of DNA microarray assays?
Signup and view all the answers
What is the purpose of applying cDNA mixture to a microarray?
Signup and view all the answers
How is gene function determined using in vitro mutagenesis?
Signup and view all the answers
What is the purpose of using RNA interference (RNAi) in gene expression studies?
Signup and view all the answers
How are genetic markers called SNPs detected?
Signup and view all the answers
What does the presence of shared SNPs among people affected with a disorder indicate?
Signup and view all the answers
The cDNA mixture is applied to a microarray to determine which genes are expressed in the tissue sample.
Signup and view all the answers
Silencing gene expression using RNA interference (RNAi) involves the use of single-stranded RNA molecules.
Signup and view all the answers
In vitro mutagenesis introduces mutations into a cloned gene, altering or enhancing its function.
Signup and view all the answers
PCR can be used to detect SNPs, which can help pinpoint the location of disease-causing genes.
Signup and view all the answers
Researchers analyze the genomes of many people with a genetic condition to identify changes specific to unaffected individuals.
Signup and view all the answers
Gel electrophoresis separates nucleic acids or proteins based on their size, electrical charge, and other properties.
Signup and view all the answers
Reverse transcriptase-polymerase chain reaction (RT-PCR) requires more mRNA than Northern blotting.
Signup and view all the answers
DNA microarray assays can only measure the expression of a few genes at one time.
Signup and view all the answers
Southern blotting combines gel electrophoresis of DNA fragments with protein hybridization.
Signup and view all the answers
Restriction fragment analysis can be used to compare two different DNA molecules if the nucleotide difference alters a restriction site.
Signup and view all the answers
