Podcast
Questions and Answers
What is the purpose of DNA cloning?
What is the purpose of DNA cloning?
- Determining the role of a gene in an organism
- Comparing genes and alleles between individuals (correct)
- Studying the expression of interacting groups of genes
- Locating gene expression in a body
What technique uses a gel as a molecular sieve to separate nucleic acids or proteins by size, electrical charge, and other properties?
What technique uses a gel as a molecular sieve to separate nucleic acids or proteins by size, electrical charge, and other properties?
- Southern Blotting
- Gel Electrophoresis (correct)
- Reverse transcriptase-polymerase chain reaction
- DNA microarray assays
Which method is used to compare mRNA from different developmental stages?
Which method is used to compare mRNA from different developmental stages?
- Southern blotting
- Northern blotting (correct)
- DNA microarray assays
- Reverse transcriptase-polymerase chain reaction
What does Southern blotting combine with gel electrophoresis of DNA fragments?
What does Southern blotting combine with gel electrophoresis of DNA fragments?
What is the purpose of DNA microarray assays?
What is the purpose of DNA microarray assays?
What is the purpose of applying cDNA mixture to a microarray?
What is the purpose of applying cDNA mixture to a microarray?
How is gene function determined using in vitro mutagenesis?
How is gene function determined using in vitro mutagenesis?
What is the purpose of using RNA interference (RNAi) in gene expression studies?
What is the purpose of using RNA interference (RNAi) in gene expression studies?
How are genetic markers called SNPs detected?
How are genetic markers called SNPs detected?
What does the presence of shared SNPs among people affected with a disorder indicate?
What does the presence of shared SNPs among people affected with a disorder indicate?
The cDNA mixture is applied to a microarray to determine which genes are expressed in the tissue sample.
The cDNA mixture is applied to a microarray to determine which genes are expressed in the tissue sample.
Silencing gene expression using RNA interference (RNAi) involves the use of single-stranded RNA molecules.
Silencing gene expression using RNA interference (RNAi) involves the use of single-stranded RNA molecules.
In vitro mutagenesis introduces mutations into a cloned gene, altering or enhancing its function.
In vitro mutagenesis introduces mutations into a cloned gene, altering or enhancing its function.
PCR can be used to detect SNPs, which can help pinpoint the location of disease-causing genes.
PCR can be used to detect SNPs, which can help pinpoint the location of disease-causing genes.
Researchers analyze the genomes of many people with a genetic condition to identify changes specific to unaffected individuals.
Researchers analyze the genomes of many people with a genetic condition to identify changes specific to unaffected individuals.
Gel electrophoresis separates nucleic acids or proteins based on their size, electrical charge, and other properties.
Gel electrophoresis separates nucleic acids or proteins based on their size, electrical charge, and other properties.
Reverse transcriptase-polymerase chain reaction (RT-PCR) requires more mRNA than Northern blotting.
Reverse transcriptase-polymerase chain reaction (RT-PCR) requires more mRNA than Northern blotting.
DNA microarray assays can only measure the expression of a few genes at one time.
DNA microarray assays can only measure the expression of a few genes at one time.
Southern blotting combines gel electrophoresis of DNA fragments with protein hybridization.
Southern blotting combines gel electrophoresis of DNA fragments with protein hybridization.
Restriction fragment analysis can be used to compare two different DNA molecules if the nucleotide difference alters a restriction site.
Restriction fragment analysis can be used to compare two different DNA molecules if the nucleotide difference alters a restriction site.
