DNA Technology and Gene Analysis Quiz
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Questions and Answers

What is the purpose of DNA cloning?

  • Determining the role of a gene in an organism
  • Comparing genes and alleles between individuals (correct)
  • Studying the expression of interacting groups of genes
  • Locating gene expression in a body
  • What technique uses a gel as a molecular sieve to separate nucleic acids or proteins by size, electrical charge, and other properties?

  • Southern Blotting
  • Gel Electrophoresis (correct)
  • Reverse transcriptase-polymerase chain reaction
  • DNA microarray assays
  • Which method is used to compare mRNA from different developmental stages?

  • Southern blotting
  • Northern blotting (correct)
  • DNA microarray assays
  • Reverse transcriptase-polymerase chain reaction
  • What does Southern blotting combine with gel electrophoresis of DNA fragments?

    <p>Nucleic acid hybridization</p> Signup and view all the answers

    What is the purpose of DNA microarray assays?

    <p>Measuring the expression of thousands of genes at one time</p> Signup and view all the answers

    What is the purpose of applying cDNA mixture to a microarray?

    <p>To visualize gene expression in the tissue sample</p> Signup and view all the answers

    How is gene function determined using in vitro mutagenesis?

    <p>By introducing mutations into a cloned gene</p> Signup and view all the answers

    What is the purpose of using RNA interference (RNAi) in gene expression studies?

    <p>To break down or block a gene’s mRNA</p> Signup and view all the answers

    How are genetic markers called SNPs detected?

    <p>By using PCR to identify single nucleotide polymorphisms</p> Signup and view all the answers

    What does the presence of shared SNPs among people affected with a disorder indicate?

    <p>The location of the disease-causing gene</p> Signup and view all the answers

    The cDNA mixture is applied to a microarray to determine which genes are expressed in the tissue sample.

    <p>True</p> Signup and view all the answers

    Silencing gene expression using RNA interference (RNAi) involves the use of single-stranded RNA molecules.

    <p>False</p> Signup and view all the answers

    In vitro mutagenesis introduces mutations into a cloned gene, altering or enhancing its function.

    <p>False</p> Signup and view all the answers

    PCR can be used to detect SNPs, which can help pinpoint the location of disease-causing genes.

    <p>True</p> Signup and view all the answers

    Researchers analyze the genomes of many people with a genetic condition to identify changes specific to unaffected individuals.

    <p>False</p> Signup and view all the answers

    Gel electrophoresis separates nucleic acids or proteins based on their size, electrical charge, and other properties.

    <p>True</p> Signup and view all the answers

    Reverse transcriptase-polymerase chain reaction (RT-PCR) requires more mRNA than Northern blotting.

    <p>False</p> Signup and view all the answers

    DNA microarray assays can only measure the expression of a few genes at one time.

    <p>False</p> Signup and view all the answers

    Southern blotting combines gel electrophoresis of DNA fragments with protein hybridization.

    <p>False</p> Signup and view all the answers

    Restriction fragment analysis can be used to compare two different DNA molecules if the nucleotide difference alters a restriction site.

    <p>True</p> Signup and view all the answers

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