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Questions and Answers
What is the percentage of thymine in the DNA sample from species X?
What is the percentage of thymine in the DNA sample from species X?
Which species has a higher guanine and cytosine content?
Which species has a higher guanine and cytosine content?
What assumption is made when calculating the base pairs in both DNA samples?
What assumption is made when calculating the base pairs in both DNA samples?
Which species is most likely to be the thermophilic bacterium and why?
Which species is most likely to be the thermophilic bacterium and why?
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What is the combined G+C content of species X?
What is the combined G+C content of species X?
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Who first isolated and characterized DNA in 1869?
Who first isolated and characterized DNA in 1869?
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Which experiment provided compelling evidence that DNA is the genetic material?
Which experiment provided compelling evidence that DNA is the genetic material?
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According to Chargaff's rules, which of the following statements is correct?
According to Chargaff's rules, which of the following statements is correct?
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What structural form do Watson and Crick propose DNA has?
What structural form do Watson and Crick propose DNA has?
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What does it mean for DNA strands to be complementary?
What does it mean for DNA strands to be complementary?
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What major discovery involved Alfred D. Hershey and Martha Chase?
What major discovery involved Alfred D. Hershey and Martha Chase?
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What did Erwin Chargaff discover about base composition in different tissues?
What did Erwin Chargaff discover about base composition in different tissues?
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Which of the following best describes the work of Oswald T. Avery?
Which of the following best describes the work of Oswald T. Avery?
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Which base pairs are held together by hydrogen bonding in DNA?
Which base pairs are held together by hydrogen bonding in DNA?
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What type of DNA structure is referred to as B-form DNA?
What type of DNA structure is referred to as B-form DNA?
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How do higher G≡C content DNA duplexes compare to those with higher A═T content?
How do higher G≡C content DNA duplexes compare to those with higher A═T content?
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What results from palindromic DNA sequences in terms of structure?
What results from palindromic DNA sequences in terms of structure?
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Which statement is true regarding mirror repeats in DNA?
Which statement is true regarding mirror repeats in DNA?
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What distinguishes the A and Z forms of DNA from B-form DNA?
What distinguishes the A and Z forms of DNA from B-form DNA?
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Which of the following is NOT a characteristic of hydrogen bonding in DNA?
Which of the following is NOT a characteristic of hydrogen bonding in DNA?
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What type of structure is formed by single-stranded palindromic DNA?
What type of structure is formed by single-stranded palindromic DNA?
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Flashcards
Chargaff's Rules
Chargaff's Rules
In DNA, adenine (A) always pairs with thymine (T), and guanine (G) always pairs with cytosine (C).
Base Pair Equivalence
Base Pair Equivalence
The percentage of adenine (A) in a DNA molecule equals the percentage of thymine (T), and the percentage of guanine (G) equals the percentage of cytosine (C).
Melting Temperature (Tm)
Melting Temperature (Tm)
The temperature at which a double-stranded DNA molecule separates into two single strands.
G+C Content and Tm
G+C Content and Tm
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Thermophilic Bacteria
Thermophilic Bacteria
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Base Pairing Rules
Base Pairing Rules
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DNA Stability Forces
DNA Stability Forces
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G≡C content and Stability
G≡C content and Stability
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B-DNA
B-DNA
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Palindromic DNA
Palindromic DNA
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Hairpin Structure
Hairpin Structure
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Cruciform Structure
Cruciform Structure
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Mirror Repeat
Mirror Repeat
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Who discovered DNA?
Who discovered DNA?
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What experiment proved DNA is the genetic material?
What experiment proved DNA is the genetic material?
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What are Chargaff's rules?
What are Chargaff's rules?
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Describe the structure of DNA.
Describe the structure of DNA.
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What are the components of a DNA nucleotide?
What are the components of a DNA nucleotide?
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Explain the concept of base pairing in DNA.
Explain the concept of base pairing in DNA.
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What is the role of base sequence in DNA?
What is the role of base sequence in DNA?
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Who proposed the double helix model of DNA?
Who proposed the double helix model of DNA?
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Study Notes
DNA Structure and History
- DNA was first isolated and characterized by Friedrich Miescher in 1869. He called the phosphorus-containing substance "nuclein."
- In the 1940s, Oswald Avery, Colin MacLeod, and Maclyn McCarty provided compelling evidence that DNA is the genetic material.
- Experiments demonstrated that DNA from virulent bacteria could transform non-virulent bacteria. This crucial step proved DNA carried genetic information.
- Further experiments by Hershey and Chase in 1952 confirmed that DNA, not protein, held genetic information.
DNA Structure
- DNA comprises two antiparallel helical polynucleotide chains.
- These chains are not identical, but instead are complementary.
- Adenine pairs with thymine, and guanine with cytosine.
- There are hydrogen bonds between these complementary base pairs.
- The two chains spiral around each other forming a right-handed double helix.
- The structure also features major and minor grooves.
Erwin Chargaff's Rule
- Erwin Chargaff's work revealed consistent base ratios across various species.
- The number of adenine bases equals the number of thymine bases and guanine equals cytosine (A=T; G=C).
- Chargaff's rules were essential for understanding DNA structure.
DNA Three-Dimensional Forms
- The Watson-Crick model, also known as B-DNA, is the most common stable form under physiological conditions.
- There are alternative forms of DNA that have been characterized: A-DNA and Z-DNA.
Comparison of A, B and Z-DNA
- Key differences exist in their dimensions (helix sense, rise per base, etc.) and conformations (C-endo and C-3' endo). The slide provides details in a table.
Palindromes and Repeats
- Palindromes are DNA sequences that are identical either forward or backward.
- These sequences can form inverted repeats, potentially leading to hairpin and cruciform structures.
- Mirror repeats occur within a single strand, unlike inverted repeats which involve both strands.
Hairpins and Cruciforms
- Palindromic sequences can lead to intrastrand base pairing.
- Hairpin structures are formed from a single strand.
- Cruciform structures involve both strands of a double helix.
DNA Base Pairs and Stability
- In two species of bacteria, adenine varied in their respective proportions.
- The proportion of G+C in the DNA correlates with the melting temperature; higher melting temperatures (higher G+C) indicate greater stability.
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Description
Test your knowledge on the structure and historical significance of DNA. This quiz covers key discoveries from Miescher to Chargaff, highlighting the crucial evidence for DNA as genetic material. Explore the specifics of its double helix configuration and base pairing.