DNA Structure and History Quiz
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Questions and Answers

What is the percentage of thymine in the DNA sample from species X?

  • 32% (correct)
  • 36%
  • 18%
  • 64%
  • Which species has a higher guanine and cytosine content?

  • Species X
  • Species Y (correct)
  • Both species have equal G+C content
  • Neither species has a G+C content above 50%
  • What assumption is made when calculating the base pairs in both DNA samples?

  • Both samples are from single-stranded DNA
  • Both DNA samples are double-stranded (correct)
  • Guanine and cytosine make up 50% each
  • Adenine and thymine percentages vary independently
  • Which species is most likely to be the thermophilic bacterium and why?

    <p>Species Y due to higher G+C content (B)</p> Signup and view all the answers

    What is the combined G+C content of species X?

    <p>36% (A)</p> Signup and view all the answers

    Who first isolated and characterized DNA in 1869?

    <p>Friedrich Miescher (A)</p> Signup and view all the answers

    Which experiment provided compelling evidence that DNA is the genetic material?

    <p>Avery and his colleagues' work with Streptococcus pneumoniae (C)</p> Signup and view all the answers

    According to Chargaff's rules, which of the following statements is correct?

    <p>A = T and G = C (B)</p> Signup and view all the answers

    What structural form do Watson and Crick propose DNA has?

    <p>A right-handed double helix (C)</p> Signup and view all the answers

    What does it mean for DNA strands to be complementary?

    <p>They pair specific bases with one another. (D)</p> Signup and view all the answers

    What major discovery involved Alfred D. Hershey and Martha Chase?

    <p>That DNA carries genetic information, not protein (B)</p> Signup and view all the answers

    What did Erwin Chargaff discover about base composition in different tissues?

    <p>It remains constant in different tissues. (D)</p> Signup and view all the answers

    Which of the following best describes the work of Oswald T. Avery?

    <p>He provided evidence supporting DNA as the genetic material. (B)</p> Signup and view all the answers

    Which base pairs are held together by hydrogen bonding in DNA?

    <p>Adenine and Thymine (B)</p> Signup and view all the answers

    What type of DNA structure is referred to as B-form DNA?

    <p>The most stable form under physiological conditions (B)</p> Signup and view all the answers

    How do higher G≡C content DNA duplexes compare to those with higher A═T content?

    <p>They are more stable (A)</p> Signup and view all the answers

    What results from palindromic DNA sequences in terms of structure?

    <p>The potential for hairpin or cruciform structures (C)</p> Signup and view all the answers

    Which statement is true regarding mirror repeats in DNA?

    <p>They cannot form cruciform structures (B)</p> Signup and view all the answers

    What distinguishes the A and Z forms of DNA from B-form DNA?

    <p>They have different geometric configurations (A)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of hydrogen bonding in DNA?

    <p>They provide significant stability to the double helix (C)</p> Signup and view all the answers

    What type of structure is formed by single-stranded palindromic DNA?

    <p>Hairpin structure (D)</p> Signup and view all the answers

    Flashcards

    Chargaff's Rules

    In DNA, adenine (A) always pairs with thymine (T), and guanine (G) always pairs with cytosine (C).

    Base Pair Equivalence

    The percentage of adenine (A) in a DNA molecule equals the percentage of thymine (T), and the percentage of guanine (G) equals the percentage of cytosine (C).

    Melting Temperature (Tm)

    The temperature at which a double-stranded DNA molecule separates into two single strands.

    G+C Content and Tm

    DNA with a higher proportion of guanine (G) and cytosine (C) base pairs has a higher melting temperature (Tm) because G-C base pairs have three hydrogen bonds, while A-T base pairs have two.

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    Thermophilic Bacteria

    Organisms that thrive in extremely hot environments, often having DNA with a high G+C content to stabilize their DNA at high temperatures.

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    Base Pairing Rules

    Adenine always pairs with thymine, and guanine always pairs with cytosine in DNA. These pairings are based on the specific shapes and chemical properties of the bases, allowing for precise hydrogen bonding between them.

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    DNA Stability Forces

    The double helix structure of DNA is stabilized by two main forces: hydrogen bonds between complementary base pairs, and base stacking interactions between adjacent bases.

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    G≡C content and Stability

    DNA with a higher G≡C content is more stable than DNA with a higher A═T content. This is because the base stacking interactions between consecutive G≡C pairs are stronger than those between A═T pairs.

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    B-DNA

    The B-form is the most common and stable form of DNA under physiological conditions. Its structure is characterized by a right-handed helix with a specific diameter and spacing between bases.

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    Palindromic DNA

    A palindrome in DNA refers to a sequence where the same base sequence is present on both strands of DNA, but in opposite directions. These sequences can form hairpins or cruciform structures.

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    Hairpin Structure

    Hairpins are single-stranded DNA or RNA structures that form when a palindromic sequence folds back on itself. These structures are stabilized by intrastrand hydrogen bonding.

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    Cruciform Structure

    Cruciform structures are formed when palindromic sequences in double-stranded DNA fold back on themselves. These structures involve both strands of the DNA and are stabilized by intrastrand hydrogen bonding.

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    Mirror Repeat

    Mirror repeats are similar to palindromes but occur within a single strand of DNA. They do not have complementary sequences within the same strand and cannot form hairpin or cruciform structures.

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    Who discovered DNA?

    Friedrich Miescher isolated and characterized a phosphorus-containing substance in 1869, calling it "nuclein." This substance was later identified as DNA.

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    What experiment proved DNA is the genetic material?

    Oswald Avery, Colin MacLeod, and Maclyn McCarty conducted experiments in the 1940s to prove that DNA, not protein, is the genetic material. They demonstrated this by transferring genetic information from a virulent strain of bacteria to a non-virulent strain using extracted DNA.

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    What are Chargaff's rules?

    Erwin Chargaff discovered that the amounts of certain nitrogenous bases in DNA are always equal (A=T, G=C). These rules guided the understanding of DNA structure and base pairing.

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    Describe the structure of DNA.

    DNA consists of two long chains of nucleotides, spiraling around a central axis. The chains run in opposite directions, creating a structure known as a double helix.

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    What are the components of a DNA nucleotide?

    Each nucleotide consists of a sugar molecule, a phosphate group, and one of four nitrogenous bases: adenine (A), thymine (T), guanine (G), and cytosine (C).

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    Explain the concept of base pairing in DNA.

    The two strands of DNA are complementary, meaning that A always pairs with T and G always pairs with C. This specific base pairing helps maintain the double helix structure and ensures accurate DNA replication.

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    What is the role of base sequence in DNA?

    The sequence of bases in a DNA strand holds the genetic information that determines an organism's traits. This information is passed on to offspring during cell division.

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    Who proposed the double helix model of DNA?

    James Watson and Francis Crick proposed the double helix model of DNA in 1953, based on the accumulated knowledge about DNA structure and Chargaff's rules, which helped revolutionize our understanding of genetics and molecular biology.

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    Study Notes

    DNA Structure and History

    • DNA was first isolated and characterized by Friedrich Miescher in 1869. He called the phosphorus-containing substance "nuclein."
    • In the 1940s, Oswald Avery, Colin MacLeod, and Maclyn McCarty provided compelling evidence that DNA is the genetic material.
    • Experiments demonstrated that DNA from virulent bacteria could transform non-virulent bacteria. This crucial step proved DNA carried genetic information.
    • Further experiments by Hershey and Chase in 1952 confirmed that DNA, not protein, held genetic information.

    DNA Structure

    • DNA comprises two antiparallel helical polynucleotide chains.
    • These chains are not identical, but instead are complementary.
    • Adenine pairs with thymine, and guanine with cytosine.
    • There are hydrogen bonds between these complementary base pairs.
    • The two chains spiral around each other forming a right-handed double helix.
    • The structure also features major and minor grooves.

    Erwin Chargaff's Rule

    • Erwin Chargaff's work revealed consistent base ratios across various species.
    • The number of adenine bases equals the number of thymine bases and guanine equals cytosine (A=T; G=C).
    • Chargaff's rules were essential for understanding DNA structure.

    DNA Three-Dimensional Forms

    • The Watson-Crick model, also known as B-DNA, is the most common stable form under physiological conditions.
    • There are alternative forms of DNA that have been characterized: A-DNA and Z-DNA.

    Comparison of A, B and Z-DNA

    • Key differences exist in their dimensions (helix sense, rise per base, etc.) and conformations (C-endo and C-3' endo). The slide provides details in a table.

    Palindromes and Repeats

    • Palindromes are DNA sequences that are identical either forward or backward.
    • These sequences can form inverted repeats, potentially leading to hairpin and cruciform structures.
    • Mirror repeats occur within a single strand, unlike inverted repeats which involve both strands.

    Hairpins and Cruciforms

    • Palindromic sequences can lead to intrastrand base pairing.
    • Hairpin structures are formed from a single strand.
    • Cruciform structures involve both strands of a double helix.

    DNA Base Pairs and Stability

    • In two species of bacteria, adenine varied in their respective proportions.
    • The proportion of G+C in the DNA correlates with the melting temperature; higher melting temperatures (higher G+C) indicate greater stability.

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    Description

    Test your knowledge on the structure and historical significance of DNA. This quiz covers key discoveries from Miescher to Chargaff, highlighting the crucial evidence for DNA as genetic material. Explore the specifics of its double helix configuration and base pairing.

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