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Questions and Answers
What is the primary structure of DNA?
What is the primary structure of DNA?
Which method involves the purification of DNA from a sample using a combination of physical and chemical methods?
Which method involves the purification of DNA from a sample using a combination of physical and chemical methods?
What is the purpose of DNA extraction?
What is the purpose of DNA extraction?
Where is DNA found in eukaryotic cells?
Where is DNA found in eukaryotic cells?
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What are the nitrogenous bases in DNA?
What are the nitrogenous bases in DNA?
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What should be added to the microfuge tube after discarding the supernatant?
What should be added to the microfuge tube after discarding the supernatant?
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What is the purpose of adding Proteinase K (PTK) to the microfuge tube?
What is the purpose of adding Proteinase K (PTK) to the microfuge tube?
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After adding Isopropanol alcohol (IPA), what is the next step in the DNA extraction procedure?
After adding Isopropanol alcohol (IPA), what is the next step in the DNA extraction procedure?
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What is the purpose of adding 300 µl of 6M NaCl after incubation in a water bath?
What is the purpose of adding 300 µl of 6M NaCl after incubation in a water bath?
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What does spinning the microfuge tube at max speed for 15 minutes help to achieve?
What does spinning the microfuge tube at max speed for 15 minutes help to achieve?
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Study Notes
Primary Structure of DNA
- DNA consists of two long strands forming a double helix structure.
- Each strand is made up of a sugar-phosphate backbone and nitrogenous bases.
DNA Purification Method
- The method to purify DNA involves both physical techniques (like centrifugation) and chemical techniques (like lysis buffers).
Purpose of DNA Extraction
- DNA extraction aims to isolate DNA from cells or tissues for various purposes, including analysis, cloning, and sequencing.
Location of DNA in Eukaryotic Cells
- In eukaryotic cells, DNA is primarily located in the nucleus.
- Small amounts of DNA are also found in mitochondria.
Nitrogenous Bases in DNA
- The nitrogenous bases in DNA include adenine (A), thymine (T), cytosine (C), and guanine (G).
- A pairs with T, and C pairs with G, forming base pairs across the double helix.
Post-Supernatant Discard Process
- After discarding the supernatant, an ethanol or isopropanol solution is typically added to help precipitate DNA.
Purpose of Proteinase K (PTK)
- Proteinase K is added to digest proteins and remove contaminants that may interfere with DNA quality.
Step After Adding Isopropanol Alcohol (IPA)
- The next step after adding IPA is typically centrifugation to precipitate the DNA into a visible pellet.
Purpose of Adding 300 µl of 6M NaCl
- Adding 6M NaCl helps to further purify the DNA by helping to precipitate proteins and other impurities.
Outcome of Spinning Microfuge Tube
- Spinning the microfuge tube at maximum speed for 15 minutes separates the DNA pellet from the liquid, consolidating the extracted DNA.
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Description
Learn about the structure and function of DNA, including its role as a carrier of genetic information in the cell. Understand the composition of DNA's primary structure and its presence in eukaryotic cells.
