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Questions and Answers
What is the primary structure of DNA?
Which method involves the purification of DNA from a sample using a combination of physical and chemical methods?
What is the purpose of DNA extraction?
Where is DNA found in eukaryotic cells?
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What are the nitrogenous bases in DNA?
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What should be added to the microfuge tube after discarding the supernatant?
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What is the purpose of adding Proteinase K (PTK) to the microfuge tube?
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After adding Isopropanol alcohol (IPA), what is the next step in the DNA extraction procedure?
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What is the purpose of adding 300 µl of 6M NaCl after incubation in a water bath?
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What does spinning the microfuge tube at max speed for 15 minutes help to achieve?
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Study Notes
Primary Structure of DNA
- DNA consists of two long strands forming a double helix structure.
- Each strand is made up of a sugar-phosphate backbone and nitrogenous bases.
DNA Purification Method
- The method to purify DNA involves both physical techniques (like centrifugation) and chemical techniques (like lysis buffers).
Purpose of DNA Extraction
- DNA extraction aims to isolate DNA from cells or tissues for various purposes, including analysis, cloning, and sequencing.
Location of DNA in Eukaryotic Cells
- In eukaryotic cells, DNA is primarily located in the nucleus.
- Small amounts of DNA are also found in mitochondria.
Nitrogenous Bases in DNA
- The nitrogenous bases in DNA include adenine (A), thymine (T), cytosine (C), and guanine (G).
- A pairs with T, and C pairs with G, forming base pairs across the double helix.
Post-Supernatant Discard Process
- After discarding the supernatant, an ethanol or isopropanol solution is typically added to help precipitate DNA.
Purpose of Proteinase K (PTK)
- Proteinase K is added to digest proteins and remove contaminants that may interfere with DNA quality.
Step After Adding Isopropanol Alcohol (IPA)
- The next step after adding IPA is typically centrifugation to precipitate the DNA into a visible pellet.
Purpose of Adding 300 µl of 6M NaCl
- Adding 6M NaCl helps to further purify the DNA by helping to precipitate proteins and other impurities.
Outcome of Spinning Microfuge Tube
- Spinning the microfuge tube at maximum speed for 15 minutes separates the DNA pellet from the liquid, consolidating the extracted DNA.
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Description
Learn about the structure and function of DNA, including its role as a carrier of genetic information in the cell. Understand the composition of DNA's primary structure and its presence in eukaryotic cells.
