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Questions and Answers
What is the process of determining the nucleotide order of a given DNA fragment called?
What is the process of determining the nucleotide order of a given DNA fragment called?
- Nucleotide synthesis
- DNA replication
- DNA sequencing (correct)
- Fragment analysis
In DNA sequencing, what technique uses sequence-specific termination of an in vitro DNA synthesis reaction using modified nucleotide substrates?
In DNA sequencing, what technique uses sequence-specific termination of an in vitro DNA synthesis reaction using modified nucleotide substrates?
- Polymerase chain reaction
- Chain termination method (correct)
- Restriction enzyme digestion
- Ligation method
Who published the chain termination method for DNA sequencing in 1977?
Who published the chain termination method for DNA sequencing in 1977?
- Franklin and Wilkins
- Sanger et al. (correct)
- Watson and Crick
- Maxam and Gilbert
What is the principle underlying chain termination DNA sequencing?
What is the principle underlying chain termination DNA sequencing?
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What is the starting material for a chain termination sequencing experiment?
What is the starting material for a chain termination sequencing experiment?
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