DNA Sequencing
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Questions and Answers

What is the process of determining the nucleotide order of a given DNA fragment called?

  • Nucleotide synthesis
  • DNA replication
  • DNA sequencing (correct)
  • Fragment analysis
  • In DNA sequencing, what technique uses sequence-specific termination of an in vitro DNA synthesis reaction using modified nucleotide substrates?

  • Polymerase chain reaction
  • Chain termination method (correct)
  • Restriction enzyme digestion
  • Ligation method
  • Who published the chain termination method for DNA sequencing in 1977?

  • Franklin and Wilkins
  • Sanger et al. (correct)
  • Watson and Crick
  • Maxam and Gilbert
  • What is the principle underlying chain termination DNA sequencing?

    <p>Separation of single-stranded DNA molecules by length</p> Signup and view all the answers

    What is the starting material for a chain termination sequencing experiment?

    <p>Identical single-stranded DNA molecules</p> Signup and view all the answers

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