DNA Sequencing and Nobel Laureates Quiz

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Questions and Answers

What was the primary function of the radioactive isotopes used in Frederick Sanger's early DNA sequencing method?

  • To label individual fragments, enabling sequence determination and overlap identification. (correct)
  • To break down the DNA into smaller fragments.
  • To accelerate the process of nucleotide binding to create longer strands of DNA.
  • To amplify the DNA in order to sequence it more efficiently.

Which key technique did Sanger's team utilize in 1977 to isolate DNA fragments for sequencing?

  • Enzymatic cleavage of DNA at specific sequences in order to identify them.
  • Radioactive tagging of DNA fragments to identify their specific sequence.
  • Gel electrophoresis using an electrical current to separate DNA.
  • Dideoxy chain-termination using a molecular inhibitor to halt DNA strand extension. (correct)

Besides sequencing both DNA and RNA, what did Sanger's work allow scientists to achieve?

  • To rapidly produce an enormous number of viral particles.
  • To create genetically modified organisms resistant to mutation.
  • To discover new DNA sequences and create modified genes with new properties. (correct)
  • To develop treatments for non-genetic disorders by targeting faulty genes.

What was the approximate time gap between Sanger sequencing the first human genome and the 3 billion total base pairs?

<p>Approximately 1 year (A)</p> Signup and view all the answers

What is the direct significance of the discovery of 48,502 base pairs of DNA?

<p>It marked a substantial event in the scientific community and an opening of new genetic sequencing techniques. (B)</p> Signup and view all the answers

Which of these positions did Lise Meitner hold during her career?

<p>Postdoctoral researcher in radioactive substances (B)</p> Signup and view all the answers

What element was discovered by Lise Meitner during her collaboration with Otto Hahn?

<p>Protactinium (B)</p> Signup and view all the answers

What was the significance of the experiment Meitner discussed with Otto Frisch?

<p>It provided evidence of uranium nuclear fission. (A)</p> Signup and view all the answers

What recognition did Otto Hahn receive for the discovery of nuclear fission?

<p>The Nobel Prize (B)</p> Signup and view all the answers

What was Enrico Fermi's major contribution to the field of nuclear physics?

<p>He created the first working nuclear chain reactor. (B)</p> Signup and view all the answers

What was the primary focus of Frederick Sanger's doctoral research?

<p>Studies into amino acids, the building blocks of proteins. (B)</p> Signup and view all the answers

Besides sequencing proteins, what other type of molecule did Sanger successfully sequence?

<p>RNA (B)</p> Signup and view all the answers

Which method of sequencing is attributed to Frederick Sanger?

<p>The dideoxy method of DNA sequencing. (C)</p> Signup and view all the answers

What was the impact of Sanger's work on the field of molecular biology?

<p>It greatly advanced medicine, gene therapy, and genetic manipulation. (A)</p> Signup and view all the answers

What influenced Sanger's strong sense of truth and conscience?

<p>His Quaker beliefs. (A)</p> Signup and view all the answers

Why was Frederick Sanger exempted from military service during WWII?

<p>He was a pacifist due to his Quaker beliefs. (C)</p> Signup and view all the answers

What discovery resulted from Sanger's work on insulin?

<p>Proteins have unique amino acid sets and 3D structures. (D)</p> Signup and view all the answers

In which year did Sanger complete the amino acid sequencing of insulin?

<p>1955 (C)</p> Signup and view all the answers

What significant conclusion did Rutherford reach about the structure of atoms?

<p>Atoms consist mainly of empty space with a dense nucleus. (C)</p> Signup and view all the answers

How did Rutherford's model of the atom differ from Thomson's model?

<p>Rutherford's model showed a nucleus at the center, while Thomson's did not. (D)</p> Signup and view all the answers

What was a key feature of Bohr's model that built upon Rutherford's findings?

<p>Electrons occupy quantized energy levels. (B)</p> Signup and view all the answers

What major scientific revolution did Rutherford's atomic model represent when it was first introduced?

<p>A radical shift in understanding that matter is mostly empty space. (B)</p> Signup and view all the answers

What was the primary reason Lise Meitner relocated from Germany to Sweden in 1938?

<p>She was escaping the increasing persecution of Jewish people under Nazi policies. (C)</p> Signup and view all the answers

What element was named in honor of Rutherford due to his significant contributions to atomic theory?

<p>Rutherfordium (B)</p> Signup and view all the answers

Lise Meitner’s doctoral thesis explored which scientific phenomenon?

<p>The movement of heat through materials with uneven particle distribution. (D)</p> Signup and view all the answers

Besides Lise Meitner, who else was awarded the Enrico Fermi Award in 1966?

<p>Otto Hahn and Fritz Strassmann (C)</p> Signup and view all the answers

Prior to attending the University of Vienna, what was the main purpose of Lise Meitner's private tutoring?

<p>To help her pass the formal entrance exam to enter university level courses. (D)</p> Signup and view all the answers

What was the specific title or focus of Meitner's very first scientific publication listed in the text?

<p>No publications are named in the contents. (D)</p> Signup and view all the answers

What was a significant contribution of George Washington Carver to agriculture?

<p>Development of alternative crops for soil restoration. (D)</p> Signup and view all the answers

Which of the following honors did George Washington Carver receive?

<p>The Springarn Medal. (D)</p> Signup and view all the answers

How many products did Carver invent from peanuts?

<p>287 (C)</p> Signup and view all the answers

In what context did Carver emphasize the importance of education?

<p>As a fundamental key to freedom and self-sufficiency. (D)</p> Signup and view all the answers

What was the outcome of Carver's methods for soil improvement and crop cultivation?

<p>Revitalization of the Southern US economy. (B)</p> Signup and view all the answers

What significant contribution did Jane Goodall make to the understanding of chimpanzee behavior?

<p>She identified the emotional and social complexities of chimpanzees. (B)</p> Signup and view all the answers

Which year did Jane Goodall earn her PhD in ethology?

<p>1965 (D)</p> Signup and view all the answers

What was the primary focus of the Roots & Shoots program created by Jane Goodall?

<p>Training young people in conservation leadership. (C)</p> Signup and view all the answers

How did Jane Goodall initially approach her studies of chimpanzees?

<p>With patience, empathy, and tenacity. (B)</p> Signup and view all the answers

What does the Jane Goodall Institute primarily focus on?

<p>Chimpanzee conservation research and education. (A)</p> Signup and view all the answers

What initial concept regarding atoms did Rutherford's research challenge?

<p>Atoms were indivisible solid spheres. (A)</p> Signup and view all the answers

Which of the following best describes the impact of Rutherford's discovery of alpha and beta radiation?

<p>It indicated that atoms were not fundamental and immutable. (C)</p> Signup and view all the answers

What specific experimental observation led Rutherford to propose the 'Rutherford model' of the atom?

<p>The deflection of alpha particles by gold foil. (C)</p> Signup and view all the answers

Why was Rutherford surprised to win a Nobel Prize in Chemistry?

<p>He believed his work was more aligned with physics. (A)</p> Signup and view all the answers

What was the key achievement that Rutherford described as 'breaking the machine and touching the ghost of matter'?

<p>The transmutation of one element into another. (A)</p> Signup and view all the answers

What role did J.J. Thomson play in Rutherford's early career?

<p>He recommended him for a professorship. (D)</p> Signup and view all the answers

What aspect of Rutherford's research directly built upon the findings of Henri Becquerel?

<p>The identification and classification of radioactive emissions. (A)</p> Signup and view all the answers

Which of the following describes the practical application of Rutherford's work on nuclear transmutation in the provided content?

<p>It showed that elements could be altered to other elements. (D)</p> Signup and view all the answers

What crucial discovery did Tak Wah Mak make in 1983?

<p>Discovered the genetic sequence and origin of T-cell receptors (D)</p> Signup and view all the answers

What specific recognition does Jocelyn Bell Burnell hold for her contributions to astronomy?

<p>Dame Commander of the British Empire (DBE) (C)</p> Signup and view all the answers

How often do pulsars emit radio waves as described in the content?

<p>Every 1.337 seconds (A)</p> Signup and view all the answers

Where did Tak Wah Mak complete his PhD?

<p>University of Alberta (A)</p> Signup and view all the answers

What characteristic defines pulsars according to their description?

<p>They are rapidly rotating, strongly magnetized neutron stars (D)</p> Signup and view all the answers

Flashcards

Nuclear Fission

A process by which a heavier atom splits into lighter atoms, releasing a significant amount of energy.

Who was Otto Hahn?

A German chemist who worked with Lise Meitner on the discovery of nuclear fission and was awarded the Nobel Prize in 1944.

Who was Otto Frisch?

Lise Meitner's nephew, a physicist who confirmed her calculations about the process of nuclear fission.

Who was Max Planck?

A prominent physicist at the University of Berlin, who gave Lise Meitner an opportunity to work with radioactive materials.

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Who was Enrico Fermi?

An Italian physicist who created the first nuclear reactor in 1942, paving the way for the development of the atomic bomb.

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Lise Meitner

Austrian theoretical nuclear physicist known for her groundbreaking work on nuclear fission.

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Theory of Nuclear Fission

The theory explaining how the nucleus of a uranium atom splits, releasing a huge amount of energy.

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Enrico Fermi Award

A prestigious award given by the US Atomic Energy Commission for outstanding achievements in the field of nuclear science.

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Heat Conduction

The ability of materials to conduct heat, similar to how electricity travels through conductors.

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Sanger Sequencing

A technique for determining the order of nucleotides in a DNA strand, where a molecular inhibitor stops DNA strand extension at specific points allowing for fragment sequencing.

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Sanger's Nobel Prizes

Frederick Sanger was awarded two Nobel Prizes in Chemistry, one in 1958 for his work on insulin and another in 1980 for his work on DNA sequencing.

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Dideoxy Chain-Termination

A crucial discovery by Sanger's team in 1977, the dideoxy chain-termination method uses a molecular inhibitor to stop DNA strands at specific points, allowing for sequencing by isolating and ordering DNA fragments.

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Sanger's Sequencing Impact

Sanger's contribution to understanding DNA sequencing revolutionized scientific research, allowing researchers to analyze and manipulate DNA sequences, potentially leading to cures for genetic disorders and other advances.

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Complete RNA Sequencing of E. coli

A significant milestone in DNA sequencing, Sanger's team was able to decipher the complete sequence of the RNA from E. coli bacteria in 1967, marking a remarkable breakthrough in understanding genetic material.

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Who was Frederick Sanger?

Frederick Sanger was a British biochemist who pioneered techniques for sequencing proteins, RNA, and DNA. He is known for his two Nobel Prizes in Chemistry - one for his work on insulin and another for his development of the dideoxy method of DNA sequencing.

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What is Sanger Sequencing?

The dideoxy method, also known as Sanger Sequencing, was developed by Frederick Sanger. It's a technique used to determine the order of nucleotides (A, T, C, G) in a DNA molecule. It involves using modified nucleotides that terminate DNA replication.

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How did Sanger Sequencing impact medicine?

Frederick Sanger's discovery of the dideoxy method has had a major impact on medicine, gene therapy, and genetic manipulation. It's crucial for understanding human diseases, developing new treatments, and diagnosing genetic disorders.

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What did Sanger win the 1958 Nobel Prize for?

In 1958, Sanger received the Nobel Prize in Chemistry for his groundbreaking work on the chemical sequencing of insulin. He showed that proteins have unique amino acid sequences and 3D structures.

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What did Sanger win the 1980 Nobel Prize for?

Frederick Sanger's second Nobel Prize in Chemistry, shared in 1980, was awarded for his DNA sequencing work. He shared the honor with Walter Gilbert and Paul Berg for their discoveries in gene technology.

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What inspired Sanger's scientific journey?

Inspired by a sense of scientific truth and driven by Quaker values, Sanger dedicated his life to scientific research. His work played a pivotal role in the development of molecular biology.

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What is the Wellcome Sanger Institute?

The Wellcome Sanger Institute, established in 1993, is a renowned research center dedicated to genomic research. It was named in honor of Frederick Sanger, who significantly contributed to the field of genomics.

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How did Sanger impact molecular biology?

Frederick Sanger was a pioneer who made significant contributions to the field of molecular biology, particularly in DNA and protein sequencing. His work has laid the foundation for advancements in gene therapy, diagnostics, and understanding of diseases.

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What is Rutherford's atomic model?

Rutherford's groundbreaking model of the atom, proposed in 1911, posits that negatively charged electrons orbit a small, dense, positively charged nucleus.

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What are the two types of radiation Rutherford identified?

Rutherford's discovery of two types of radiation emitted from radioactive elements - Alpha and Beta.

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What are the characteristics of Alpha and Beta radiation?

Alpha radiation consists of positively charged particles, much heavier than electrons. Beta radiation is made up of negatively charged particles, similar to electrons.

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What is the first transmutation of elements?

Rutherford's groundbreaking achievement of transforming one element into another, marking a significant milestone in nuclear physics.

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How did Rutherford achieve the first transmutation?

Rutherford's experiment using alpha particle bombardment to transform nitrogen into oxygen, providing evidence for nuclear fission and the release of protons.

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What is nuclear fission?

The splitting of an atom's nucleus, releasing energy and resulting in the formation of different elements.

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What is a proton?

The positively charged particle found in the nucleus of an atom, discovered by Rutherford in 1919.

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Who is Ernest Rutherford?

Ernest Rutherford, a New Zealand-born physicist, revolutionized our understanding of the atom and radioactivity, winning the Nobel Prize in Chemistry for his research on the disintegration of elements.

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Rutherford's Model

Rutherford's model of the atom, proposed in 1911, describes the atom as a miniature solar system with electrons orbiting a tiny, dense, positively charged nucleus.

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Rutherford's Gold Foil Experiment

Ernest Rutherford's experiment in 1911 revolutionized our understanding of the atom. He fired positively charged particles at a thin layer of gold atoms. The results showed that most particles passed through the atom, some were deflected, and a few bounced back. This led to the discovery of the nucleus, a tiny, dense, positively charged center.

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Thomson's 'Plum Pudding' Model

This model was proposed earlier by J.J. Thomson, suggesting that atoms are like plum pudding, with positive charge spread throughout and electrons embedded within. Rutherford's experiment disproved it.

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Bohr Model of the Atom

Niels Bohr, working with Rutherford, further refined our understanding of the atom by introducing the concept of quantized energy levels for electrons. Electrons can only occupy specific energy levels.

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Atomic Nucleus

The atomic nucleus is the tiny, dense, positively charged center of an atom. It is composed of protons, which are positively charged particles, and neutrons, which carry no charge. The nucleus holds almost all of the atom's mass.

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What are pulsars?

Pulsars are highly magnetized, rotating neutron stars that emit radio waves in beams. These beams create pulses of radiation that make them appear to be blinking.

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What did Jocelyn Bell Burnell discover?

Jocelyn Bell Burnell discovered pulsars while conducting research with radio telescopes, which led to a new understanding of neutron stars.

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What did Tak Wah Mak study in immunology?

Tak Wah Mak's research focused on T-cells, which are crucial for the immune system's ability to fight infections and recognize antigens. He discovered the origin and sequence of T-cell receptors, which are responsible for recognizing antigens.

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How did Tak Wah Mak's research help fight cancer?

Tak Wah Mak's contributions to immunology helped to identify a chemical that can block the fuel supply of Hodgkin's lymphoma, a type of cancer.

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What did Tak Wah Mak's discoveries lead to?

Tak Wah Mak's impressive discoveries led to him being recognized as one of the leading experts in the field of immunology.

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Who was George Washington Carver?

George Washington Carver was a prominent African American agricultural scientist who played a crucial role in restoring the Southern US economy after the Civil War.

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How did Carver contribute to the Southern economy?

Carver's research on soil improvement and alternative crops like peanuts, sweet potatoes, and soybeans revitalized the South's agricultural economy. These crops were crucial for restoring nitrogen-depleted soil from cotton farming.

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What were some of Carver's significant discoveries?

Carver's groundbreaking research led to the development of over 400 marketable products from peanuts and sweet potatoes, including oils and dyes. This boosted the South's agricultural output and economic returns.

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What was Carver's philosophy on education?

Carver strongly believed in the transformative power of education. He emphasized that it empowers individuals and unlocks opportunities for success.

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What was Carver's legacy?

Carver dedicated his life to improving agricultural practices and empowering African Americans. He taught sustainable farming methods at Tuskegee Institute for 47 years, advocating for self-reliance and economic growth.

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Who is Jane Goodall?

Jane Goodall is a renowned British primatologist who is best known for her groundbreaking research on chimpanzees, especially their social behavior and intelligence.

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What makes Jane Goodall's work so significant?

Goodall's extensive research on chimpanzees in Tanzania revealed remarkable similarities between their behavior and that of humans, including tool use, social structures, and complex emotions.

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What is the Jane Goodall Institute and what does it do?

The Jane Goodall Institute, founded in 1977, serves as a global organization dedicated to protecting chimpanzees and their habitats through conservation efforts, research, and education.

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What was one of Goodall's most groundbreaking discoveries about chimpanzees?

One of Goodall's most significant observations was that chimpanzees use tools, like sticks to gather food, demonstrating a level of intelligence and problem-solving skills previously attributed solely to humans.

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How did Goodall achieve such insightful observations of chimpanzees?

Goodall's approach to studying chimpanzees involved natural patience, empathy, and tenacity, allowing her to gain their trust and observe their behavior in detail.

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