DNA Replication Process

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Questions and Answers

What is the main purpose of DNA replication during cell division?

  • To repair DNA damage in cells
  • To ensure the formation of two identical daughter cells (correct)
  • To increase genetic diversity in daughter cells
  • To form different daughter cells

Why is DNA replication considered semi-conservative?

  • Each new DNA has both old strands from the original DNA.
  • Each new DNA has two completely new strands.
  • Each new DNA has only one old strand.
  • Each new DNA has one old and one new strand. (correct)

What is the function of pre-replication complex proteins like ORC and MCM?

  • They are responsible for breaking down the DNA strands.
  • They are involved in DNA repair mechanisms.
  • They regulate cell cycle checkpoints.
  • They recognize origins of replication and recruit DNA replication machinery. (correct)

In which direction does DNA synthesis always proceed?

<p>5'→3' (C)</p> Signup and view all the answers

Why do DNA polymerases require a primer to initiate chain synthesis?

<p>To begin chain growth as they cannot initiate de novo synthesis (C)</p> Signup and view all the answers

Where does DNA replication start in eukaryotic cells?

<p>At pre-replication complex recognition sites (D)</p> Signup and view all the answers

Which enzyme is responsible for adding deoxynucleotides to the free hydroxyl group at the 3′ end of the primer during DNA replication?

<p>Polymerases (A)</p> Signup and view all the answers

What is the function of Primases in DNA replication?

<p>To form the RNA primers required for DNA polymerases (B)</p> Signup and view all the answers

Which enzyme is essential in unwinding the parental DNA strands during DNA replication?

<p>Helicases (B)</p> Signup and view all the answers

What is the function of Topoisomerases during DNA replication?

<p>To relieve tensions created by the replication bubble (B)</p> Signup and view all the answers

Which enzyme is responsible for ligation of different strands that are being formed, especially in the lagging strand?

<p>Ligases (A)</p> Signup and view all the answers

What complex is involved in synthesizing the leading strand during DNA replication?

<p>Rfc (Replication factors) (B)</p> Signup and view all the answers

What is the function of the clamp loader in DNA replication?

<p>Opening the PCNA ring to load onto DNA (B)</p> Signup and view all the answers

What is the role of DNA polymerase-alpha in primer synthesis during DNA replication?

<p>Adding DNA to the RNA primer (C)</p> Signup and view all the answers

Which enzyme is responsible for polymerizing the lagging strand during DNA replication?

<p>DNA polymerase δ (Pol δ) (C)</p> Signup and view all the answers

What is the main function of the complex formed by DNA polymerase δ (Pol δ)-PCNA-Rfc in DNA replication?

<p>Polymerizing the lagging strand (D)</p> Signup and view all the answers

What is a common feature of all telomeres that relates to their orientation on the chromosome?

<p>3'-end made up of a G-rich single-stranded region (B)</p> Signup and view all the answers

During DNA replication, what does the term 'concurrent' refer to?

<p>Simultaneous synthesis of leading and lagging strands in both replication forks (A)</p> Signup and view all the answers

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