DNA Replication Process Overview
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Questions and Answers

What is the enzymatic activity of helicases during DNA replication?

  • Forming RNA primers
  • Polymerizing nucleotides
  • Relieving tensions created by the replication bubble
  • Unwinding the parental DNA strands (correct)

    • Which complex prevents the whole DNA polymerase ε complex from falling off the template during DNA replication?

  • RPA
  • RFC
  • PCNA (correct)
  • Primase

    • What is the function of DNA ligases in the process of DNA replication?

  • Unwinding the parental DNA strands
  • Protecting single-strand DNA from nucleases
  • Polymerizing nucleotides
  • Ligating the different strands being formed (correct)

    • Which enzyme is responsible for adding deoxynucleotides to the free hydroxyl group at the 3′ end of the primer during DNA replication?

    <p>DNA polymerase</p> Signup and view all the answers

    What is the main function of primases in DNA replication?

    <p>Forming RNA primers</p> Signup and view all the answers

    What type of replication is observed in DNA replication?

    <p>Semi-continuous in one strand</p> Signup and view all the answers

    What is the function of the clamp loader in DNA replication?

    <p>Opens the PCNA ring to load onto DNA</p> Signup and view all the answers

    Which enzyme is responsible for synthesizing short RNA primers in DNA replication?

    <p>RNA primase</p> Signup and view all the answers

    What is the function of DNA polymerase-alpha in DNA replication?

    <p>Adds DNA to the RNA primer</p> Signup and view all the answers

    What is the main function of DNA ligases in DNA replication?

    <p>Joining Okazaki fragments together</p> Signup and view all the answers

    Which part of a chromosome makes up the G-tail at the 3'-end?

    <p>Terminal portion of the G-rich strand</p> Signup and view all the answers

    What is a common feature of all telomeres?

    <p>Single-stranded G-tail</p> Signup and view all the answers

    Which direction does DNA synthesis always proceed during replication?

    <p>5' -&gt; 3'</p> Signup and view all the answers

    Which enzyme is responsible for the formation of Okazaki fragments?

    <p>Primase</p> Signup and view all the answers

    What is the function of DNA ligase in DNA replication?

    <p>Sealing nicks in the sugar-phosphate backbone</p> Signup and view all the answers

    During DNA replication, which enzyme is responsible for unwinding the DNA double helix?

    <p>Helicase</p> Signup and view all the answers

    What is the role of DNA polymerase III in DNA replication?

    <p>Main enzyme for leading strand synthesis</p> Signup and view all the answers

    In DNA replication, which enzyme is required to join Okazaki fragments together?

    <p>DNA ligase</p> Signup and view all the answers

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