DNA Replication Process Overview

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Questions and Answers

What is the role of the DnaA protein in DNA replication?

  • Causing DNA to stretch and separating the strands (correct)
  • Loading the DnaB helicase onto the unwound DNA
  • Binding to the single-stranded regions to prevent rejoining of the strands
  • Breaking the hydrogen bonds between nitrogenous base pairs

What is the function of the DnaB helicase in DNA replication?

  • Forming a prepriming complex with the DnaA protein
  • Binding single-stranded DNA-binding proteins (SSBP)
  • Continuing the unwinding of DNA by breaking hydrogen bonds (correct)
  • Preventing the strands from rejoining

What is the function of single-strand DNA-binding protein (SSBP) in DNA replication?

  • Loading the DnaB helicase onto the unwound DNA
  • Breaking the hydrogen bonds between nitrogenous base pairs
  • Forming a prepriming complex with DnaA protein
  • Preventing rejoining of the DNA strands (correct)

What are replication forks in DNA replication?

<p>Junctions between double-stranded and unwound single-stranded DNA (A)</p> Signup and view all the answers

What is the function of the DnaB helicase in DNA replication?

<p>Continuing the unwinding of DNA by breaking hydrogen bonds (D)</p> Signup and view all the answers

What is the role of single-strand DNA-binding protein (SSBP) in DNA replication?

<p>Binding to single-stranded regions to prevent strands from rejoining (A)</p> Signup and view all the answers

What happens as more DnaA proteins bind to the unwound DNA?

<p>DnaA proteins are released from the DNA (A)</p> Signup and view all the answers

What defines a replication bubble in DNA replication?

<p>Region where DNA appears like a bubble (D)</p> Signup and view all the answers

What is the primary function of DnaC helicase loader proteins?

<p>Helping load DnaB helicase onto unwound DNA (C)</p> Signup and view all the answers

How many replication forks are formed at the origin of replication?

<p>Two (A)</p> Signup and view all the answers

What is the significance of the replication forks in DNA replication?

<p>They move in opposite directions (C)</p> Signup and view all the answers

Where do DnaA proteins and DnaB-DnaC complex bind during DNA replication?

<p>At the origin of replication (C)</p> Signup and view all the answers

What prevents the DNA strands from rejoining during replication?

<p>Single-strand DNA-binding protein (SSBP) (C)</p> Signup and view all the answers

Which protein is responsible for continuing the unwinding of DNA by breaking hydrogen bonds?

<p>DnaB helicase (B)</p> Signup and view all the answers

Flashcards

Role of DnaA protein?

Causes DNA to stretch and separates the strands, initiating DNA replication.

Function of DnaB helicase?

Continues unwinding of DNA by breaking hydrogen bonds between base pairs.

Role of single-strand DNA-binding protein (SSBP)?

Prevents rejoining of separated DNA strands by binding to single-stranded regions.

What are replication forks?

Junctions formed between double-stranded DNA and unwound single-stranded DNA during replication.

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What defines a replication bubble?

Region where DNA appears like a bubble, indicating unwinding and active replication.

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Function of DnaC helicase loader proteins?

Helps load DnaB helicase onto unwound DNA at the origin of replication.

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How many forks are formed?

There are two replication forks that move in opposite directions.

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Significance of replication forks?

They move in opposite directions, allowing efficient and complete replication of the DNA.

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What prevents DNA strands from rejoining?

Single-strand DNA-binding protein (SSBP).

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