DNA Replication in E. coli and Vicia faba

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10 Questions

What provides energy for polymerisation reactions in DNA replication?

Deoxynucleoside triphosphates

In which direction does DNA-dependent DNA polymerase catalyze polymerisation?

5' to 3'

What term is used to describe the small opening in the DNA helix where replication occurs?

Replication fork

What enzyme joins the discontinuously synthesized DNA fragments?

DNA ligase

What term is used to describe the region in E. coli DNA where replication initiates?

Origin of replication

What enzyme is referred to as DNA-dependent DNA polymerase?

DNA-dependent DNA polymerase

What is the average rate of polymerization in E.coli during DNA replication?

2000 bp per second

What would result from any mistake during DNA replication?

Mutations

What dual purpose do deoxyribonucleoside triphosphates serve during replication?

Serve as building blocks for DNA synthesis and provide energy for the process

What did the experiments on Vicia faba (faba beans) by Taylor and colleagues in 1958 prove?

DNA in chromosomes replicates semiconservatively

Explore the replication of DNA in E. coli and Vicia faba through experimental findings. Learn about the proportions of light and hybrid densities DNA molecules after a specific growth period and how radioactive thymidine was used to detect newly synthesized DNA in chromosomes.

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