DNA Replication and Repair Quiz

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Transcription

The process by which genetic information in DNA is copied into messenger RNA (mRNA) for protein synthesis.

Excision Repair

A mechanism that removes and replaces damaged DNA, such as nucleotide excision repair, which corrects UV-induced damage.

Translation

The process by which ribosomes convert the genetic information in mRNA into a sequence of amino acids to build proteins.

Mismatch Repair

A system for recognizing and repairing erroneous insertion, deletion, and mis-incorporation of bases during DNA replication and recombination.

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Codon

A sequence of three nucleotides in mRNA that codes for a specific amino acid or signals the end of protein synthesis.

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PCR (Polymerase Chain Reaction)

A laboratory technique used to make multiple copies of a segment of DNA.

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Aminoacyl-tRNA Synthetase

An enzyme that attaches the appropriate amino acid onto its tRNA.

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Sanger Sequencing

A method of DNA sequencing based on the selective incorporation of chain-terminating dideoxynucleotides during DNA replication.

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Genetic Code

The set of rules by which information encoded in mRNA sequences is translated into proteins by living cells.

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DNA Polymerase

An enzyme that synthesizes DNA molecules from deoxyribonucleotides, essential for DNA replication.

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Anticodon

A sequence of three bases in tRNA that is complementary to a codon in mRNA.

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Proofreading

The function of DNA polymerase to correct errors in DNA synthesis by removing and replacing incorrect nucleotides.

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Silent Mutation

A mutation that changes a single nucleotide, but does not change the amino acid created, thus having no effect on the protein's function.

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Introns

Non-coding sequences of DNA within a gene that are removed during RNA processing.

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tRNA (Transfer RNA)

A type of RNA molecule that helps decode mRNA sequences into proteins during translation by carrying amino acids to the ribosome.

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Rosalind Franklin

An English chemist whose X-ray diffraction images of DNA contributed to the discovery of the DNA double helix structure.

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Okazaki fragment

Short sequences of DNA nucleotides which are synthesized discontinuously and later linked together on the lagging strand during DNA replication.

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Watson and Crick

James Watson and Francis Crick, who, along with Maurice Wilkins and using Rosalind Franklin's data, discovered the double helix structure of DNA.

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ORI (Origin of Replication)

A specific sequence in the genome where DNA replication begins.

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Transformation

The genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings.

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Leading Strand

The strand of DNA that is synthesized continuously during DNA replication.

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Chargaff

Erwin Chargaff, a biochemist known for Chargaff's rules, which state that DNA from any cell should have a 1:1 ratio of pyrimidine and purine bases (A=T and C=G).

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Telomerase

An enzyme that adds nucleotides to the ends of chromosomes (telomeres) to prevent them from shortening during replication.

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Helicase

An enzyme that unwinds the DNA helix ahead of the replication fork.

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Lagging Strand

The strand of DNA that is synthesized discontinuously in short sections (Okazaki fragments) during DNA replication.

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Template

A strand of DNA or RNA used as a guide to synthesize a complementary strand.

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Plasmid

Small, circular, double-stranded DNA molecules that are separate from a cell's chromosomal DNA and can replicate independently. Commonly found in bacteria.

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Antiparallel

Refers to the opposite orientation of the two strands of DNA in the double helix, where one strand runs 5' to 3' and the other 3' to 5'.

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Semi-conservative

The method of DNA replication in which each new double helix contains one old strand and one new strand.

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Primer

A short nucleic acid sequence that provides a starting point for DNA synthesis.

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Study Notes

DNA Replication and Repair

  • Transcription: Copies genetic information from DNA into messenger RNA (mRNA) for protein production.
  • Excision Repair: Removes and replaces damaged DNA, like UV-induced damage.
  • Translation: Ribosomes convert mRNA into amino acid sequences forming proteins.
  • Mismatch Repair: Fixes errors in DNA replication, like insertion, deletion, and mis-incorporation of bases.
  • Codon: Three nucleotides in mRNA that code for an amino acid or stop signal.
  • PCR (Polymerase Chain Reaction): Creates multiple copies of a DNA segment.
  • Aminoacyl-tRNA Synthetase: Attaches the right amino acid to its tRNA.
  • Sanger Sequencing: Method of DNA sequencing using chain-terminating dideoxynucleotides.
  • Genetic Code: Rules for translating mRNA sequences into proteins.
  • DNA Polymerase: Enzyme synthesizing DNA from deoxyribonucleotides (essential for replication).
  • Anticodon: tRNA sequence complementary to a codon in mRNA.
  • Proofreading: DNA polymerase's error-correction mechanism; removes incorrect nucleotides.
  • Silent Mutation: Nucleotide change with no effect on the amino acid sequence.
  • Introns: Non-coding DNA sequences removed from RNA during processing.
  • tRNA (Transfer RNA): Carries amino acids to the ribosome during translation.
  • Rosalind Franklin: Her X-ray diffraction images helped discover the double helix.
  • Okazaki Fragment: Short DNA segments synthesized discontinuously on the lagging strand.
  • Watson and Crick: Discovered the double helix structure of DNA, drawing on Rosalind Franklin's data.
  • ORI (Origin of Replication): DNA sequence where replication starts.
  • Transformation: Acquiring exogenous genetic material from surrounding environment.
  • Leading Strand: DNA strand synthesized continuously during replication.

DNA Structure and Function

  • Chargaff's Rules: DNA from any cell has a 1:1 ratio of A=T and C=G.
  • Telomerase: Adds nucleotides to chromosome ends to prevent shortening.
  • Helicase: Enzyme unwinding the DNA helix during replication.
  • Lagging Strand: Synthesized discontinuously in short sections (Okazaki fragments).
  • Template: DNA or RNA strand used as a guide for complementary strand synthesis.
  • Plasmid: Small, circular, double-stranded DNA molecules.
  • Antiparallel: Opposite 5' to 3' orientations of DNA strands in the double helix.
  • Semi-conservative: In DNA replication, each new double helix contains one old and one new strand.
  • Primer: Short nucleic acid sequence starting DNA synthesis.

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