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Questions and Answers
What is the purpose of nucleotide excision repair?
What is the purpose of nucleotide excision repair?
- To remove mis-incorporated bases during DNA replication
- To correct thymine dimers induced by UV light (correct)
- To synthesize and fill gaps in DNA strands
- To recognize and cleave damaged strands
What is the name of the UV specific endonuclease that recognizes and cleaves the damaged strand?
What is the name of the UV specific endonuclease that recognizes and cleaves the damaged strand?
- Exonuclease 1
- Ligase
- uvrABC excinuclease (correct)
- MutS
What is the purpose of mismatch repair?
What is the purpose of mismatch repair?
- To correct thymine dimers induced by UV light
- To remove mis-incorporated bases during DNA replication (correct)
- To synthesize and fill gaps in DNA strands
- To recognize and cleave damaged strands
How many major proteins are involved in the MMR system in E. coli?
How many major proteins are involved in the MMR system in E. coli?
Which protein in the MMR system recognizes the mismatch and recruits MutL?
Which protein in the MMR system recognizes the mismatch and recruits MutL?
What does MutH do in the MMR system?
What does MutH do in the MMR system?
What does Exonuclease 1 do in the MMR system?
What does Exonuclease 1 do in the MMR system?
What does DNA polymerase III do in the MMR system?
What does DNA polymerase III do in the MMR system?
What is the purpose of Ligase in the MMR system?
What is the purpose of Ligase in the MMR system?
Which type of damage does nucleotide excision repair remove?
Which type of damage does nucleotide excision repair remove?
Which protein recruits MutH in the MMR system?
Which protein recruits MutH in the MMR system?
What happens after Ligase seals the gap in the MMR system?
What happens after Ligase seals the gap in the MMR system?
What is the purpose of nucleotide excision repair?
What is the purpose of nucleotide excision repair?
What is the name of the UV specific endonuclease that recognizes and cleaves the damaged strand?
What is the name of the UV specific endonuclease that recognizes and cleaves the damaged strand?
What is the purpose of mismatch repair?
What is the purpose of mismatch repair?
How many major proteins are involved in the MMR system in E. coli?
How many major proteins are involved in the MMR system in E. coli?
Which protein in the MMR system recognizes the mismatch and recruits MutL?
Which protein in the MMR system recognizes the mismatch and recruits MutL?
What does MutH do in the MMR system?
What does MutH do in the MMR system?
What does Exonuclease 1 do in the MMR system?
What does Exonuclease 1 do in the MMR system?
What does DNA polymerase III do in the MMR system?
What does DNA polymerase III do in the MMR system?
What is the purpose of Ligase in the MMR system?
What is the purpose of Ligase in the MMR system?
Which type of damage does nucleotide excision repair remove?
Which type of damage does nucleotide excision repair remove?
Which protein recruits MutH in the MMR system?
Which protein recruits MutH in the MMR system?
What happens after Ligase seals the gap in the MMR system?
What happens after Ligase seals the gap in the MMR system?
What is the purpose of nucleotide excision repair?
What is the purpose of nucleotide excision repair?
Which endonuclease is responsible for recognizing and cleaving the damaged strand during nucleotide excision repair?
Which endonuclease is responsible for recognizing and cleaving the damaged strand during nucleotide excision repair?
What is the purpose of mismatch repair?
What is the purpose of mismatch repair?
Which protein is responsible for recognizing the mismatch during MMR?
Which protein is responsible for recognizing the mismatch during MMR?
What is the role of MutL during MMR?
What is the role of MutL during MMR?
Which endonuclease cuts the DNA strand flanking the mismatch during MMR?
Which endonuclease cuts the DNA strand flanking the mismatch during MMR?
What is the role of Exonuclease 1 during MMR?
What is the role of Exonuclease 1 during MMR?
Which protein is responsible for synthesizing and filling the gap during MMR?
Which protein is responsible for synthesizing and filling the gap during MMR?
What is the purpose of ligase during MMR?
What is the purpose of ligase during MMR?
How many major proteins are involved in the MMR system in E. coli?
How many major proteins are involved in the MMR system in E. coli?
What is the name of the endonuclease responsible for recognizing and cleaving the damaged strand during nucleotide excision repair?
What is the name of the endonuclease responsible for recognizing and cleaving the damaged strand during nucleotide excision repair?
Which repair system corrects mis-incorporated bases during DNA replication?
Which repair system corrects mis-incorporated bases during DNA replication?
Flashcards
Nucleotide excision repair
Nucleotide excision repair
A repair pathway that corrects errors in DNA caused by UV radiation.
uvrABC excinuclease
uvrABC excinuclease
A specific endonuclease that recognizes and cleaves DNA strands damaged by UV radiation, specifically thymine dimers.
Mismatch repair
Mismatch repair
The repair pathway that removes incorrectly incorporated bases during DNA replication.
MutS
MutS
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MutL
MutL
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MutH
MutH
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Exonuclease 1
Exonuclease 1
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DNA polymerase III
DNA polymerase III
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Ligase
Ligase
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Thymine dimers
Thymine dimers
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3 major proteins in MMR
3 major proteins in MMR
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What does nucleotide excision repair do?
What does nucleotide excision repair do?
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What is the name of the UV-specific endonuclease?
What is the name of the UV-specific endonuclease?
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What is the purpose of mismatch repair?
What is the purpose of mismatch repair?
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Which protein recognizes the mismatch?
Which protein recognizes the mismatch?
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What does MutL do?
What does MutL do?
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Which protein cuts the DNA strand?
Which protein cuts the DNA strand?
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What does Exonuclease 1 do?
What does Exonuclease 1 do?
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What does DNA polymerase III do?
What does DNA polymerase III do?
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What does Ligase do?
What does Ligase do?
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What happens after Ligase acts?
What happens after Ligase acts?
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Which type of damage does nucleotide excision repair remove?
Which type of damage does nucleotide excision repair remove?
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Which protein recruits MutH?
Which protein recruits MutH?
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What happens after Ligase seals the gap?
What happens after Ligase seals the gap?
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Purpose of nucleotide excision repair
Purpose of nucleotide excision repair
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Which endonuclease cleaves the damaged strand in nucleotide excision repair?
Which endonuclease cleaves the damaged strand in nucleotide excision repair?
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Purpose of mismatch repair
Purpose of mismatch repair
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Which protein recognizes the mismatch in mismatch repair?
Which protein recognizes the mismatch in mismatch repair?
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Role of MutL in mismatch repair
Role of MutL in mismatch repair
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Which endonuclease cuts the DNA strand in mismatch repair?
Which endonuclease cuts the DNA strand in mismatch repair?
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Role of Exonuclease 1 in mismatch repair
Role of Exonuclease 1 in mismatch repair
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Which protein fills the gap in mismatch repair?
Which protein fills the gap in mismatch repair?
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Purpose of ligase in mismatch repair
Purpose of ligase in mismatch repair
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Number of proteins in mismatch repair
Number of proteins in mismatch repair
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Name of the endonuclease in nucleotide excision repair
Name of the endonuclease in nucleotide excision repair
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Which system corrects replication errors?
Which system corrects replication errors?
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Study Notes
Nucleotide Excision Repair
- Purpose: to remove DNA damage, specifically bulky lesions and UV-induced damage
- Uses UV-specific endonuclease (UvrABC) to recognize and cleave the damaged strand
- Removes helix-distorting DNA lesions, including UV-induced cyclobutane pyrimidine dimers
Mismatch Repair
- Purpose: to correct mis-incorporated bases during DNA replication
- Involves 5 major proteins in E. coli: MutS, MutL, MutH, Exonuclease 1, and DNA polymerase III
- MutS recognizes the mismatch and recruits MutL
- MutH cuts the DNA strand flanking the mismatch
- Exonuclease 1 removes the mismatched DNA strand
- DNA polymerase III synthesizes and fills the gap
- Ligase seals the gap
- After Ligase seals the gap, the corrected DNA strand is restored
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Description
Test your knowledge on DNA repair mechanisms with this quiz! From nucleotide excision repair to mismatch repair, this quiz will challenge your understanding of how cells fix errors in DNA replication. Brush up on the key proteins and enzymes involved in these processes and discover new insights into the fascinating world of DNA repair. Perfect for biology students, researchers, and science enthusiasts.
