10 Questions
What is the function of primase in DNA replication?
To synthesize a small RNA primer
What is DNA polymerase III?
A holoenzyme
What is the function of the polymerase core unit of DNA polymerase III?
To synthesize DNA
Which enzyme activity is possessed by the 3'-5' exonuclease subunit of DNA polymerase III?
Proofreading activity
Which end of the RNA primer does DNA polymerase III use to synthesize the complementary DNA strand?
3' end
What types of nucleotide triphosphates are added by the 5'-3' polymerase subunit of DNA polymerase III?
dATP, dTTP, dGTP & dCTP
What is released when a deoxy nucleotide triphosphate is added to the elongating chain by the 5'-3' polymerase subunit of DNA polymerase III?
Two phosphate molecules (pyrophosphate)
What type of bond is created between the incoming nucleotide and the last primer nucleotide by the 5'-3' polymerase subunit of DNA polymerase III?
Phosphodiester bond
What is the end product of the synthesis of dCTP by DNA polymerase III?
Cytidine monophosphate (dCMP; incorporated into DNA)
What is the function of the 3'-5' exonuclease subunit of DNA polymerase III?
To remove incorrect nucleotides from the newly synthesized DNA strand
Test Your Knowledge on DNA Polymerase III and Its Functions. Learn about the DNA polymerase III holoenzyme, its 10 functional subunits, and how it uses RNA primers to synthesize complementary DNA strands. Brush up on the different enzyme activities of the polymerase core unit, including 5'-3' DNA polymerase and 3'-5' exonuclease. Take this quiz and see how much you know about DNA replication and DNA polymerase III.
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