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DNA Polymerase III Quiz

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Questions and Answers

What is the function of primase in DNA replication?

To synthesize a small DNA primer
To proofread the newly synthesized DNA
To synthesize a small RNA primer (correct)
To remove RNA primers

What is DNA polymerase III?

A trimer
A monomer
A holoenzyme (correct)
A dimer

What is the function of the polymerase core unit of DNA polymerase III?

To proofread DNA
To remove RNA primers
To initiate DNA replication
To synthesize DNA (correct)

Which enzyme activity is possessed by the 3'-5' exonuclease subunit of DNA polymerase III?

Proofreading activity (C) Signup and view all the answers

Which end of the RNA primer does DNA polymerase III use to synthesize the complementary DNA strand?

3' end (B) Signup and view all the answers

What types of nucleotide triphosphates are added by the 5'-3' polymerase subunit of DNA polymerase III?

dATP, dTTP, dGTP & dCTP (C) Signup and view all the answers

What is released when a deoxy nucleotide triphosphate is added to the elongating chain by the 5'-3' polymerase subunit of DNA polymerase III?

Two phosphate molecules (pyrophosphate) (D) Signup and view all the answers

What type of bond is created between the incoming nucleotide and the last primer nucleotide by the 5'-3' polymerase subunit of DNA polymerase III?

Phosphodiester bond (B) Signup and view all the answers

What is the end product of the synthesis of dCTP by DNA polymerase III?

Cytidine monophosphate (dCMP; incorporated into DNA) (C) Signup and view all the answers

What is the function of the 3'-5' exonuclease subunit of DNA polymerase III?

To remove incorrect nucleotides from the newly synthesized DNA strand (C) Signup and view all the answers