DNA Mutations and Modifying Agents
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Questions and Answers

What is the term used to describe chemicals that insert themselves between DNA bases and can lead to frameshift mutations?

  • Thymine dimers
  • Intercalating agents (correct)
  • DNA-modifying agents
  • Chemical mutagens
  • What type of mutation leads to the insertion of an extra nucleotide in the codon sequence?

  • Transitions mutation
  • Suppressor mutation
  • Reversion mutation
  • Deletion mutation (correct)
  • Which type of mutant expresses its phenotype only under certain conditions?

  • Resistance mutant
  • Lethal mutant
  • Conditional mutant (correct)
  • Biochemical mutant
  • Which type of mutation alters a single nucleotide base but does not change the amino acid sequence in the protein produced?

    <p>Silent mutation</p> Signup and view all the answers

    What is the term for a mutant unable to grow in the absence of a specific molecule?

    <p>Auxotroph</p> Signup and view all the answers

    What is the effect of a nonsense mutation in the genetic code?

    <p>It results in a faulty protein with a wrong amino acid sequence.</p> Signup and view all the answers

    Which type of mutation results in a stop codon, halting the translation process?

    <p>Nonsense mutation</p> Signup and view all the answers

    Which phenomenon can restore the original gene by reversing a previously occurred mutation?

    <p>Suppressor mutation</p> Signup and view all the answers

    What type of mutant undergoes a mutation that reverts back to the wild-type phenotype at a secondary site?

    <p>Suppressor mutant</p> Signup and view all the answers

    What is the term for the observable characteristics of an organism as a result of its genetic makeup?

    <p>Phenotype</p> Signup and view all the answers

    Which chemical agent is known for chemically altering DNA bases, such as guanine binding to thymine?

    <p>Methyl-nitrosoguanidine</p> Signup and view all the answers

    What is the function of a suppressor tRNA with a mutation?

    <p>Synthesizes wild type despite the mutation</p> Signup and view all the answers

    How is the process of screening different from selection?

    <p>Selection involves transferring cells to selection and non-selection, while screening involves looking through colonies for non-selectable qualities.</p> Signup and view all the answers

    What is the role of LexA in the Global SOS response?

    <p>Prevents gene expression when not needed</p> Signup and view all the answers

    What is the main advantage of horizontal gene transfer?

    <p>Transfers genetic material between unrelated organisms</p> Signup and view all the answers

    How does RecA protein contribute to gene repair?

    <p>Repairs chromosome breaks and damages LexA</p> Signup and view all the answers

    Which enzyme is responsible for preventing gene expression in the absence of damage?

    <p>LexA repressor</p> Signup and view all the answers

    What is the protein that facilitates strand invasion during DNA exchange or recombination?

    <p>RecA</p> Signup and view all the answers

    Which form of DNA can carry antibiotic resistance genes and other genes?

    <p>Plasmids</p> Signup and view all the answers

    What allows for the movement of jumping genes known as transposons within DNA?

    <p>Transposase enzyme</p> Signup and view all the answers

    What is responsible for creating double-strand breaks during the process of transferring DNA?

    <p>Transposase enzyme</p> Signup and view all the answers

    Which element consists of two identical IS elements with genes in between and can carry antibiotic resistance genes?

    <p>Transposable element</p> Signup and view all the answers

    During DNA exchange, what must be similar in order for homologous recombination to occur?

    <p>Flanking regions of DNA</p> Signup and view all the answers

    What process involves the transfer of free DNA fragments?

    <p>Transformation</p> Signup and view all the answers

    Which element recognizes the IS element ends in transposition?

    <p>Transposable gene</p> Signup and view all the answers

    What is the function of RepA in plasmids during bacterial conjugation?

    <p>Unwinds the replication single strand</p> Signup and view all the answers

    Which cycle involves phage DNA integrating into the bacterial chromosome at a homologous site?

    <p>Lysogenic Cycle</p> Signup and view all the answers

    What is the function of a prophage in the lysogenic cycle?

    <p>Initiating a new lytic cycle</p> Signup and view all the answers

    Which method involves the transfer of DNA directly between bacteria through direct cell-to-cell contact?

    <p>Conjugation</p> Signup and view all the answers

    In rolling circle replication, what binds to the origin of replication (ori)?

    <p>RepA</p> Signup and view all the answers

    What type of transduction brings DNA from the first host cell into the recipient cell?

    <p>Generalized transduction</p> Signup and view all the answers

    What facilitates transfer during bacterial conjugation?

    <p>Tra operon</p> Signup and view all the answers

    In specialized transduction, what exchanges a portion of host DNA between cells?

    <p>Transducing particle</p> Signup and view all the answers

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