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Questions and Answers
What is the primary function of DNA ligase in the cloning process?
What is the primary function of DNA ligase in the cloning process?
- It helps to unwind the DNA strands
- It facilitates the replication of DNA molecules
- It separates the DNA into smaller fragments
- It covalently joins DNA fragments together (correct)
What does the term 'cloning' refer to in the context of DNA cloning?
What does the term 'cloning' refer to in the context of DNA cloning?
- Introducing a foreign gene into a host organism
- Creating a genetically modified organism
- Making several copies of a DNA molecule (correct)
- Replicating a DNA molecule in a test tube
What is a recombinant DNA molecule?
What is a recombinant DNA molecule?
- A DNA molecule that has been synthesized in a laboratory
- A DNA molecule that has been cloned from a living organism
- A DNA molecule that contains genetic material from two different sources (correct)
- A DNA molecule that has been modified to express a specific protein
What is the primary advantage of using plasmids as cloning vectors?
What is the primary advantage of using plasmids as cloning vectors?
Which of the following is a required component of a plasmid cloning vector?
Which of the following is a required component of a plasmid cloning vector?
What is the purpose of including a selectable marker gene, such as an antibiotic resistance gene, in a plasmid cloning vector?
What is the purpose of including a selectable marker gene, such as an antibiotic resistance gene, in a plasmid cloning vector?
What technique allows the separation of proteins based on their size?
What technique allows the separation of proteins based on their size?
Which of the following protein tags is a fluorescent protein that can be detected by its fluorescence?
Which of the following protein tags is a fluorescent protein that can be detected by its fluorescence?
What technique involves the use of antibodies to purify a specific protein from a mixture?
What technique involves the use of antibodies to purify a specific protein from a mixture?
Which of the following protein tags allows for purification using beads with bound nickel ions?
Which of the following protein tags allows for purification using beads with bound nickel ions?
What technique involves the transfer of separated proteins from a gel to a membrane for detection with antibodies?
What technique involves the transfer of separated proteins from a gel to a membrane for detection with antibodies?
Which of the following protein tags can be purified using a peptide corresponding to its amino acid sequence?
Which of the following protein tags can be purified using a peptide corresponding to its amino acid sequence?
What is a protein domain?
What is a protein domain?
What is the purpose of Green Fluorescent Protein (GFP) in proteins?
What is the purpose of Green Fluorescent Protein (GFP) in proteins?
What are the characteristics of a protein domain?
What are the characteristics of a protein domain?
What does GST stand for in the context of proteins?
What does GST stand for in the context of proteins?
Why are recombinant proteins produced?
Why are recombinant proteins produced?
What is the purpose of a restriction site in the creation of recombinant DNA?
What is the purpose of a restriction site in the creation of recombinant DNA?
What is the purpose of a DNA ligase in the creation of recombinant DNA?
What is the purpose of a DNA ligase in the creation of recombinant DNA?
What is the purpose of a promoter sequence in an expression vector?
What is the purpose of a promoter sequence in an expression vector?
What is the purpose of a poly-T primer in the reverse transcription of mRNA?
What is the purpose of a poly-T primer in the reverse transcription of mRNA?
Why is it challenging to express human proteins in bacteria?
Why is it challenging to express human proteins in bacteria?
What is the purpose of protein tagging in recombinant protein expression?
What is the purpose of protein tagging in recombinant protein expression?
What is the primary purpose of DNA cloning?
What is the primary purpose of DNA cloning?
What are restriction endonucleases?
What are restriction endonucleases?
What type of sequences are recognized by restriction endonucleases?
What type of sequences are recognized by restriction endonucleases?
How do restriction endonucleases cut DNA?
How do restriction endonucleases cut DNA?
What is a vector in DNA cloning?
What is a vector in DNA cloning?
What are the products of a staggered (off-center) cut by a restriction endonuclease?
What are the products of a staggered (off-center) cut by a restriction endonuclease?
Which of the following protein tags allows for purification using beads with bound nickel ions?
Which of the following protein tags allows for purification using beads with bound nickel ions?
What technique involves the transfer of separated proteins from a gel to a membrane for detection with antibodies?
What technique involves the transfer of separated proteins from a gel to a membrane for detection with antibodies?
Which of the following protein tags can be purified using a peptide corresponding to its amino acid sequence?
Which of the following protein tags can be purified using a peptide corresponding to its amino acid sequence?
What is the purpose of the FLAG tag in protein purification?
What is the purpose of the FLAG tag in protein purification?
Which of the following protein tags is a fluorescent protein that can be detected by its fluorescence?
Which of the following protein tags is a fluorescent protein that can be detected by its fluorescence?
What technique allows the separation of proteins based on their size?
What technique allows the separation of proteins based on their size?
What technique involves the use of antibodies to purify a specific protein from a mixture?
What technique involves the use of antibodies to purify a specific protein from a mixture?
What is the main purpose of DNA cloning?
What is the main purpose of DNA cloning?
What is the key function of restriction endonucleases in DNA cloning?
What is the key function of restriction endonucleases in DNA cloning?
What type of DNA ends are generated by a staggered (off-center) cut by a restriction endonuclease?
What type of DNA ends are generated by a staggered (off-center) cut by a restriction endonuclease?
What is the defining characteristic of the DNA sequences recognized by restriction endonucleases?
What is the defining characteristic of the DNA sequences recognized by restriction endonucleases?
What type of DNA ends are generated by a blunt cut by a restriction endonuclease?
What type of DNA ends are generated by a blunt cut by a restriction endonuclease?
What is the primary advantage of using plasmids as cloning vectors in DNA cloning?
What is the primary advantage of using plasmids as cloning vectors in DNA cloning?
What is the main purpose of including a selectable marker gene, such as an antibiotic resistance gene, in a plasmid cloning vector?
What is the main purpose of including a selectable marker gene, such as an antibiotic resistance gene, in a plasmid cloning vector?
What is the role of DNA ligase in cloning?
What is the role of DNA ligase in cloning?
Why are plasmids considered good vectors for cloning?
Why are plasmids considered good vectors for cloning?
What is the key feature that plasmid cloning vectors must possess?
What is the key feature that plasmid cloning vectors must possess?
What is the purpose of a selectable gene in a plasmid cloning vector?
What is the purpose of a selectable gene in a plasmid cloning vector?
What is recombinant DNA technology part of?
What is recombinant DNA technology part of?
Which statement best describes a clone?
Which statement best describes a clone?
What is the key step in creating recombinant DNA using vectors?
What is the key step in creating recombinant DNA using vectors?
What is the role of DNA ligase in the creation of recombinant DNA?
What is the role of DNA ligase in the creation of recombinant DNA?
What is the primary function of a poly-T primer in reverse transcription?
What is the primary function of a poly-T primer in reverse transcription?
Why are eukaryotic systems like yeast preferred for protein expression over bacteria?
Why are eukaryotic systems like yeast preferred for protein expression over bacteria?
What is the main advantage of using protein tagging in recombinant protein expression?
What is the main advantage of using protein tagging in recombinant protein expression?
In the context of gene expression vectors, what do ShineDalgarno (SD) sequences function as?
In the context of gene expression vectors, what do ShineDalgarno (SD) sequences function as?
What is the primary purpose of utilizing the same restriction endonuclease to cut both DNA fragments during cloning?
What is the primary purpose of utilizing the same restriction endonuclease to cut both DNA fragments during cloning?
Why is plasmid used as a vector in DNA cloning?
Why is plasmid used as a vector in DNA cloning?
What is the primary function of a protein domain?
What is the primary function of a protein domain?
What is the main purpose of using a GST-tag in recombinant protein production?
What is the main purpose of using a GST-tag in recombinant protein production?
What is the primary advantage of using a GFP-tag compared to a GST-tag in recombinant protein production?
What is the primary advantage of using a GFP-tag compared to a GST-tag in recombinant protein production?
Which of the following is a key characteristic of a protein domain?
Which of the following is a key characteristic of a protein domain?
What is the primary purpose of producing recombinant proteins?
What is the primary purpose of producing recombinant proteins?
What is the main advantage of using plasmids as cloning vectors?
What is the main advantage of using plasmids as cloning vectors?
What is the purpose of a promoter sequence in an expression vector?
What is the purpose of a promoter sequence in an expression vector?
Which of the following is a required component of a plasmid cloning vector?
Which of the following is a required component of a plasmid cloning vector?
What is the primary function of DNA ligase in the cloning process?
What is the primary function of DNA ligase in the cloning process?
What is the purpose of including a selectable marker gene, such as an antibiotic resistance gene, in a plasmid cloning vector?
What is the purpose of including a selectable marker gene, such as an antibiotic resistance gene, in a plasmid cloning vector?
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