DNA Extraction and Purification Quiz
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Questions and Answers

What is the purpose of the Dische test in DNA extraction and purification?

  • To isolate and extract cellular DNA from the sample
  • To identify and purify the extracted DNA using restriction enzymes
  • To confirm the presence of DNA by reacting with a deoxyribose sugar (correct)
  • To measure the intensity of absorbance of the DNA solution at 260 nm and 280 nm

    • What does a 260/280 ratio measurement indicate in DNA purity check?

  • The conversion of 2-deoxyribose to hydroxylevulinyl aldehyde
  • The presence of protein contamination in the DNA preparation
  • The relative absence of protein contamination in the DNA preparation (correct)
  • The confirmation of DNA through chemical hydrolysis

    • What is the role of restriction endonucleases in molecular analysis of genomic DNA?

  • To confirm the presence of DNA by reacting with a deoxyribose sugar
  • To cleave double-stranded (ds) DNA into smaller, more manageable fragments (correct)
  • To measure the intensity of absorbance of the DNA solution at different wavelengths
  • To isolate and extract cellular DNA from the sample

    • What is the specific purpose of the Dische test in DNA extraction and purification?

    <p>To confirm the presence of DNA by using a diphenylamine (DPA) indicator</p> Signup and view all the answers

    What property of DNA does the Dische test specifically confirm?

    <p>Presence of deoxyribose sugar</p> Signup and view all the answers

    What does measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicate?

    <p>The purity of DNA</p> Signup and view all the answers

    What is the ideal 260/280 ratio for a pure sample of DNA?

    <p>1.8</p> Signup and view all the answers

    What does a 260/280 ratio lower than 1.8 indicate in a DNA preparation?

    <p>Lower purity with minimal protein contamination</p> Signup and view all the answers

    Which enzyme is responsible for cleaving double-stranded DNA into smaller, more manageable fragments?

    <p>Restriction endonucleases (restriction enzymes)</p> Signup and view all the answers

    What is the purpose of the Dische test in DNA extraction and purification?

    <p>To confirm the presence of DNA by producing a blue-colored compound</p> Signup and view all the answers

    What does a 260/280 ratio lower than 1.8 indicate in a DNA preparation?

    <p>Presence of aromatic proteins</p> Signup and view all the answers

    What is the ideal 260/280 ratio for a pure sample of DNA?

    <p>1.8</p> Signup and view all the answers

    What property of DNA does the Dische test specifically confirm?

    <p>Presence</p> Signup and view all the answers

    Which step involves chemical hydrolysis of DNA to produce a blue-colored compound?

    <p>Dische test</p> Signup and view all the answers

    What is used to measure the intensity of absorbance of the DNA solution in DNA purity check?

    <p>$260 nm wavelength light</p> Signup and view all the answers

    What does measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicate?

    <p>$260/280 ratio</p> Signup and view all the answers

    Which enzyme cleaves double-stranded DNA into smaller, more manageable fragments?

    <p>$Restriction endonucleases</p> Signup and view all the answers

    What is the specific action of restriction endonucleases in molecular analysis of genomic DNA?

    <p>$Cleavage of dsDNA into smaller fragments</p> Signup and view all the answers

    What confirms the presence of DNA by reacting with deoxyribose to produce a blue-colored compound?

    <p>$Diphenylamine</p> Signup and view all the answers

    What is used to measure absorbance intensity at wavelengths 260 nm and 280 nm to assess DNA purity?

    <p>Light</p> Signup and view all the answers

    In DNA purity check, what is used as a measure of DNA purity?

    <p>$260/280 ratio</p> Signup and view all the answers

    Study Notes

    DNA Extraction and Purification

    • The Dische test is used to confirm the presence of DNA by reacting with deoxyribose to produce a blue-colored compound.
    • The Dische test specifically confirms the presence of DNA by reacting with deoxyribose.

    DNA Purity Check

    • Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicates the purity of the DNA sample.
    • A 260/280 ratio measurement indicates the purity of the DNA sample, with a ratio of 1.8 or higher indicating a pure sample.
    • A 260/280 ratio lower than 1.8 indicates contamination or impurities in the DNA preparation.
    • The ideal 260/280 ratio for a pure sample of DNA is 1.8 or higher.
    • Spectrophotometry is used to measure the intensity of absorbance of the DNA solution in DNA purity check.

    Restriction Endonucleases

    • Restriction endonucleases are enzymes that cleave double-stranded DNA into smaller, more manageable fragments.
    • The specific action of restriction endonucleases in molecular analysis of genomic DNA is to cleave double-stranded DNA into smaller fragments.

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    Related Documents

    10-DNA Extraction and Purification.ppt

    Description

    Test your knowledge about the extraction, isolation, and identification of cellular DNA, as well as the action of restriction enzymes and methods for purity check.

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