21 Questions
What is the purpose of the Dische test in DNA extraction and purification?
To confirm the presence of DNA by reacting with a deoxyribose sugar
What does a 260/280 ratio measurement indicate in DNA purity check?
The relative absence of protein contamination in the DNA preparation
What is the role of restriction endonucleases in molecular analysis of genomic DNA?
To cleave double-stranded (ds) DNA into smaller, more manageable fragments
What is the specific purpose of the Dische test in DNA extraction and purification?
To confirm the presence of DNA by using a diphenylamine (DPA) indicator
What property of DNA does the Dische test specifically confirm?
Presence of deoxyribose sugar
What does measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicate?
The purity of DNA
What is the ideal 260/280 ratio for a pure sample of DNA?
1.8
What does a 260/280 ratio lower than 1.8 indicate in a DNA preparation?
Lower purity with minimal protein contamination
Which enzyme is responsible for cleaving double-stranded DNA into smaller, more manageable fragments?
Restriction endonucleases (restriction enzymes)
What is the purpose of the Dische test in DNA extraction and purification?
To confirm the presence of DNA by producing a blue-colored compound
What does a 260/280 ratio lower than 1.8 indicate in a DNA preparation?
Presence of aromatic proteins
What is the ideal 260/280 ratio for a pure sample of DNA?
1.8
What property of DNA does the Dische test specifically confirm?
Presence
Which step involves chemical hydrolysis of DNA to produce a blue-colored compound?
Dische test
What is used to measure the intensity of absorbance of the DNA solution in DNA purity check?
$260 nm wavelength light
What does measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicate?
$260/280 ratio
Which enzyme cleaves double-stranded DNA into smaller, more manageable fragments?
$Restriction endonucleases
What is the specific action of restriction endonucleases in molecular analysis of genomic DNA?
$Cleavage of dsDNA into smaller fragments
What confirms the presence of DNA by reacting with deoxyribose to produce a blue-colored compound?
$Diphenylamine
What is used to measure absorbance intensity at wavelengths 260 nm and 280 nm to assess DNA purity?
Light
In DNA purity check, what is used as a measure of DNA purity?
$260/280 ratio
Study Notes
DNA Extraction and Purification
- The Dische test is used to confirm the presence of DNA by reacting with deoxyribose to produce a blue-colored compound.
- The Dische test specifically confirms the presence of DNA by reacting with deoxyribose.
DNA Purity Check
- Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicates the purity of the DNA sample.
- A 260/280 ratio measurement indicates the purity of the DNA sample, with a ratio of 1.8 or higher indicating a pure sample.
- A 260/280 ratio lower than 1.8 indicates contamination or impurities in the DNA preparation.
- The ideal 260/280 ratio for a pure sample of DNA is 1.8 or higher.
- Spectrophotometry is used to measure the intensity of absorbance of the DNA solution in DNA purity check.
Restriction Endonucleases
- Restriction endonucleases are enzymes that cleave double-stranded DNA into smaller, more manageable fragments.
- The specific action of restriction endonucleases in molecular analysis of genomic DNA is to cleave double-stranded DNA into smaller fragments.
Test your knowledge about the extraction, isolation, and identification of cellular DNA, as well as the action of restriction enzymes and methods for purity check.
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