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Questions and Answers
What is the purpose of the Dische test in DNA extraction and purification?
What is the purpose of the Dische test in DNA extraction and purification?
- To isolate and extract cellular DNA from the sample
- To identify and purify the extracted DNA using restriction enzymes
- To confirm the presence of DNA by reacting with a deoxyribose sugar (correct)
- To measure the intensity of absorbance of the DNA solution at 260 nm and 280 nm
What does a 260/280 ratio measurement indicate in DNA purity check?
What does a 260/280 ratio measurement indicate in DNA purity check?
- The conversion of 2-deoxyribose to hydroxylevulinyl aldehyde
- The presence of protein contamination in the DNA preparation
- The relative absence of protein contamination in the DNA preparation (correct)
- The confirmation of DNA through chemical hydrolysis
What is the role of restriction endonucleases in molecular analysis of genomic DNA?
What is the role of restriction endonucleases in molecular analysis of genomic DNA?
- To confirm the presence of DNA by reacting with a deoxyribose sugar
- To cleave double-stranded (ds) DNA into smaller, more manageable fragments (correct)
- To measure the intensity of absorbance of the DNA solution at different wavelengths
- To isolate and extract cellular DNA from the sample
What is the specific purpose of the Dische test in DNA extraction and purification?
What is the specific purpose of the Dische test in DNA extraction and purification?
What property of DNA does the Dische test specifically confirm?
What property of DNA does the Dische test specifically confirm?
What does measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicate?
What does measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicate?
What is the ideal 260/280 ratio for a pure sample of DNA?
What is the ideal 260/280 ratio for a pure sample of DNA?
What does a 260/280 ratio lower than 1.8 indicate in a DNA preparation?
What does a 260/280 ratio lower than 1.8 indicate in a DNA preparation?
Which enzyme is responsible for cleaving double-stranded DNA into smaller, more manageable fragments?
Which enzyme is responsible for cleaving double-stranded DNA into smaller, more manageable fragments?
What is the purpose of the Dische test in DNA extraction and purification?
What is the purpose of the Dische test in DNA extraction and purification?
What does a 260/280 ratio lower than 1.8 indicate in a DNA preparation?
What does a 260/280 ratio lower than 1.8 indicate in a DNA preparation?
What is the ideal 260/280 ratio for a pure sample of DNA?
What is the ideal 260/280 ratio for a pure sample of DNA?
What property of DNA does the Dische test specifically confirm?
What property of DNA does the Dische test specifically confirm?
Which step involves chemical hydrolysis of DNA to produce a blue-colored compound?
Which step involves chemical hydrolysis of DNA to produce a blue-colored compound?
What is used to measure the intensity of absorbance of the DNA solution in DNA purity check?
What is used to measure the intensity of absorbance of the DNA solution in DNA purity check?
What does measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicate?
What does measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicate?
Which enzyme cleaves double-stranded DNA into smaller, more manageable fragments?
Which enzyme cleaves double-stranded DNA into smaller, more manageable fragments?
What is the specific action of restriction endonucleases in molecular analysis of genomic DNA?
What is the specific action of restriction endonucleases in molecular analysis of genomic DNA?
What confirms the presence of DNA by reacting with deoxyribose to produce a blue-colored compound?
What confirms the presence of DNA by reacting with deoxyribose to produce a blue-colored compound?
What is used to measure absorbance intensity at wavelengths 260 nm and 280 nm to assess DNA purity?
What is used to measure absorbance intensity at wavelengths 260 nm and 280 nm to assess DNA purity?
In DNA purity check, what is used as a measure of DNA purity?
In DNA purity check, what is used as a measure of DNA purity?
Study Notes
DNA Extraction and Purification
- The Dische test is used to confirm the presence of DNA by reacting with deoxyribose to produce a blue-colored compound.
- The Dische test specifically confirms the presence of DNA by reacting with deoxyribose.
DNA Purity Check
- Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm indicates the purity of the DNA sample.
- A 260/280 ratio measurement indicates the purity of the DNA sample, with a ratio of 1.8 or higher indicating a pure sample.
- A 260/280 ratio lower than 1.8 indicates contamination or impurities in the DNA preparation.
- The ideal 260/280 ratio for a pure sample of DNA is 1.8 or higher.
- Spectrophotometry is used to measure the intensity of absorbance of the DNA solution in DNA purity check.
Restriction Endonucleases
- Restriction endonucleases are enzymes that cleave double-stranded DNA into smaller, more manageable fragments.
- The specific action of restriction endonucleases in molecular analysis of genomic DNA is to cleave double-stranded DNA into smaller fragments.
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Description
Test your knowledge about the extraction, isolation, and identification of cellular DNA, as well as the action of restriction enzymes and methods for purity check.
