BIO 222 Chap 10 DNA and Genetic Material

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Questions and Answers

What key characteristic must genetic material possess to ensure continuity of life?

  • The presence of a simple, repetitive structure.
  • The ability to catalyze metabolic reactions.
  • The capacity to vary and undergo mutations. (correct)
  • The ability to directly interact with cellular membranes.

Why was the elucidation of DNA's structure so pivotal in genetics?

  • It enabled the direct manipulation of genes for therapeutic purposes.
  • It revealed how genetic information could be encoded and expressed. (correct)
  • It confirmed DNA as the sole carrier of genetic information.
  • It simplified genetic studies by providing a uniform model.

What discovery is Johann Friedrich Miescher credited with?

  • Determining the base pairing rules in DNA.
  • Identifying the double helix structure of DNA.
  • Isolating and characterizing nuclein from cell nuclei. (correct)
  • Proposing the tetranucleotide theory of DNA composition.

According to Chargaff's rules, if a double-stranded DNA molecule consists of 28% guanine, what percentage of adenine would it contain?

<p>22% (D)</p> Signup and view all the answers

What role did Levene's work play in advancing our understanding of DNA structure?

<p>He discovered the basic components of a nucleotide. (A)</p> Signup and view all the answers

What critical finding emerged from Griffith's experiments with Streptococcus pneumoniae?

<p>A transforming principle can change non-virulent bacteria into virulent bacteria. (B)</p> Signup and view all the answers

During the Avery-MacLeod-McCarty experiment, what evidence led them to determine DNA, rather than protein, was the transforming principle?

<p>Digesting DNA extract with DNase prevented transformation. (C)</p> Signup and view all the answers

Suppose Avery, MacLeod, and McCarty found that heat-killed bacteria samples treated with both RNase and protease still transformed bacteria, but samples treated with DNase did not. What assertion would that support?

<p>DNA is the genetic material. (B)</p> Signup and view all the answers

In the Hershey-Chase experiment, why was radioactive phosphorus (⁻²P) used to label DNA?

<p>Phosphorus is found in DNA but not typically in proteins. (A)</p> Signup and view all the answers

In the Hershey-Chase experiment designed to determine the carrier of genetic information, if both radioactive sulfur and radioactive phosphorus were found inside the infected bacteria, how would that affect the conclusion?

<p>It would invalidate the experiment's design, making the conclusion uncertain. (D)</p> Signup and view all the answers

What critical role did Rosalind Franklin's work play in the discovery of DNA's structure?

<p>Providing key X-ray diffraction images of DNA. (B)</p> Signup and view all the answers

What type of data did Watson and Crick primarily use to construct their model of DNA?

<p>X-ray diffraction data and base composition rules. (B)</p> Signup and view all the answers

How is genetic information typically encoded in most organisms?

<p>DNA. (C)</p> Signup and view all the answers

Which of the following distinguishes RNA from DNA?

<p>RNA contains ribose sugar, while DNA contains deoxyribose sugar. (B)</p> Signup and view all the answers

A nucleotide consists of what three components?

<p>A sugar, a phosphate group, and a nitrogenous base. (D)</p> Signup and view all the answers

What type of bond connects the nucleotides in a single strand of DNA?

<p>Phosphodiester bond. (D)</p> Signup and view all the answers

Which base is typically found in RNA but not in DNA?

<p>Uracil. (A)</p> Signup and view all the answers

What is the key feature of DNA's secondary structure?

<p>The double helix with complementary, antiparallel strands. (A)</p> Signup and view all the answers

What contributes to the stability of the DNA double helix?

<p>Hydrophobic interactions between the bases. (C)</p> Signup and view all the answers

What does the term 'antiparallel' refer to in the context of DNA structure?

<p>The opposite 5' to 3' direction of the two DNA strands. (A)</p> Signup and view all the answers

If one strand of DNA has the sequence 5'-ATGCTGA-3', what is the sequence of the complementary strand?

<p>5'-CAGCTAT-3' (D)</p> Signup and view all the answers

How many hydrogen bonds are typically formed between adenine and thymine in a DNA molecule?

<p>Two. (C)</p> Signup and view all the answers

What characterizes the B-DNA form, which is the standard form described by Watson and Crick?

<p>A right-handed helix with a smooth backbone. (C)</p> Signup and view all the answers

Under what circumstances can a hairpin structure form within a single strand of RNA or DNA?

<p>When the strand has sequences that are inverted complements. (B)</p> Signup and view all the answers

A DNA sequence reads 5'-GGCCAATTGG-3'. Which sequence could form a hairpin structure within this strand?

<p>5'-CCAATTAAGG-3' (A)</p> Signup and view all the answers

What is H-DNA characterized by?

<p>A triple-stranded structure formed by DNA unwinding. (C)</p> Signup and view all the answers

Where are sequences likely to form H-DNA typically found?

<p>Mammalian genomes. (A)</p> Signup and view all the answers

What role does DNA methylation play in biological systems?

<p>It alters DNA's three-dimensional structure and affects gene expression. (D)</p> Signup and view all the answers

Where are methyl groups added to modify DNA?

<p>Nitrogenous bases. (B)</p> Signup and view all the answers

How does knowledge of DNA structure inform treatments for genetic diseases?

<p>It allows designing therapies that target specific sequences or structures. (C)</p> Signup and view all the answers

How do the unique structures formed by DNA and RNA, such as hairpins and H-DNA, impact cellular processes?

<p>They can affect gene expression and genome stability. (A)</p> Signup and view all the answers

How does the fact that DNA is the genetic material influence our understanding of human evolution?

<p>It helps us to understand mutations and heredity patterns. (C)</p> Signup and view all the answers

What is the main characteristic of genetic material?

<p>It must encode the phenotype. (A)</p> Signup and view all the answers

During Miescher experiment, which cells were they experimenting with?

<p>White Blood cells. (C)</p> Signup and view all the answers

Which viruses encode the genetic information in RNA instead of DNA?

<p>Tobacco mosaic virus. (A)</p> Signup and view all the answers

If Griffith's experiment had shown that the heat-killed virulent bacteria did not transform the nonvirulent bacteria into a virulent form, how would this have affected the subsequent search for the genetic material?

<p>It would have indicated that transformation requires a living agent. (C)</p> Signup and view all the answers

In the Hershey-Chase experiment, what would the conclusions have been if the radioactive sulfur (³⁵S) was found in the infected bacteria and the radioactive phosphorus (³²P) was found in the phage ghosts?

<p>It would have weakened the conclusion that DNA is the genetic material, suggesting protein might also play a role. (D)</p> Signup and view all the answers

Which experimental finding would most strongly support the conclusion that RNA, not DNA, can be the genetic material?

<p>Discovering a virus whose genome is made entirely of RNA and is infectious. (D)</p> Signup and view all the answers

Considering the structure of DNA, which characteristic is most directly related to its ability to store vast amounts of genetic information?

<p>The specific sequence of nucleotide bases. (D)</p> Signup and view all the answers

A mutation occurs in a cell, preventing the formation of hydrogen bonds in DNA. What would be the most likely consequence of this mutation?

<p>Disruption of DNA replication and transcription. (D)</p> Signup and view all the answers

How would the disruption of phosphodiester bonds in a single strand of DNA directly impact DNA's function?

<p>It would lead to the fragmentation of the DNA strand. (A)</p> Signup and view all the answers

A scientist discovers a new virus with a unique nucleic acid composition: adenine makes up 15%, guanine 35%, cytosine 20%, and uracil 30%. What can be concluded about the virus's genetic material?

<p>The virus has a single-stranded RNA genome. (D)</p> Signup and view all the answers

How does DNA methylation alter gene expression?

<p>By affecting the binding of proteins to DNA. (A)</p> Signup and view all the answers

What implications would the discovery of a protein with the ability to destabilize H-DNA structures have on cellular processes?

<p>It would affect gene expression patterns. (B)</p> Signup and view all the answers

A researcher is studying a genetic sequence in a mammalian cell and observes a region forming H-DNA. What might the researcher infer about this region's function?

<p>It likely contains repetitive sequences and regulates nearby gene expression. (A)</p> Signup and view all the answers

Flashcards

Genetic Material

Genetic material must contain a large amount of complex information.

Genetic Replication

Genetic material must accurately duplicate itself.

Genetic Encoding

Genetic material must have the capacity to encode phenotypes.

Genetic Variation

Genetic material must have the potential for variation.

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Miescher's Contribution

Miescher isolated a weakly acidic, phosphorus-rich substance from cell nuclei, now known as DNA

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Chargaff's Rules

Chargaff's rules state that in DNA, the amount of adenine is equal to thymine and guanine is equal to cytosine.

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Levene Contributions

Levene determined that DNA consists of a string of nucleotides

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Transformation

The process by which external DNA is taken up by a cell, changing its characteristics.

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Griffith's Experiment

Griffith's experiments demonstrated a transforming principle, where heat-killed virulent bacteria could transform live nonvirulent bacteria.

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Avery-MacLeod-McCarty Experiment

Avery, MacLeod, and McCarty demonstrated that DNA is the transforming principle, not protein or RNA.

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Hershey-Chase Experiment

Hershey and Chase demonstrated that DNA, not protein, is the genetic material in bacteriophages using radioactive labeling.

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X-ray Diffraction

A technique in which X-rays are directed at a molecule in crystal form; the diffraction patterns produced are used to determine the molecule's structure.

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Watson and Crick

Watson and Crick developed the three-dimensional structure of DNA using X-ray diffraction data and model building.

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RNA as Genetic Material

In most organisms, DNA carries genetic information; however, in some viruses, RNA carries genetic information instead.

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DNA Primary Structure

Primary structure of DNA refers to its nucleotide sequence.

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Deoxyribonucleotide Components

Deoxyribonucleotides consist of a sugar, a phosphate, and a base.

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DNA Secondary Structure

The secondary structure of DNA involves the double helix, hydrogen bonds, and base pairing.

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Hydrogen Bonds in DNA

Hydrogen bonds hold the two strands of DNA together through base pairing.

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DNA Three-Dimensional Structure

Three-dimensional structure of DNA identified by Watson and Crick refers to B-DNA.

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Hairpin DNA Structure

In single strands of nucleotides, when sequences of nucleotides on the same strand are inverted complements, a hairpin structure will be formed.

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H-DNA structure

H-DNA is a three-stranded (triplex) structure formed when DNA unwinds and one strand pairs with double-stranded DNA from another part of the molecule

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H-DNA sequence

H-DNA occurs often in long sequences of only purines or pyrimidines

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DNA methylation

DNA methylation add methyl groups to nucleotide bases

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Study Notes

Genetic Material Characteristics

  • Genetic material contains complex information, replicates faithfully, encodes the phenotype, and has the capacity to vary.
  • The discovery of DNA's structure was essential for understanding how genetic information is encoded and expressed.

Encoding Genetic Information

  • Early DNA studies include Miescher's discovery of nuclein, Kossel's identification of four nitrogenous bases in DNA, and Chargaff's rules.
  • Chargaff's rules state that adenine (A) always equals thymine (T), and guanine (G) always equals cytosine (C).
  • The Griffith experiment identified a transforming principle.
  • Avery, MacLeod, and McCarty's experiment revealed that DNA is the transforming principle.
  • The Hershey-Chase experiment confirmed that DNA carries genetic information in bacteriophages.
  • Watson and Crick discovered the three-dimensional structure of DNA, partly based on X-ray diffraction images.
  • In most organisms, DNA carries genetic information, but in some viruses, RNA carries genetic information instead, for example the tobacco mosaic virus.

DNA Structure

  • Primary DNA structure consists of deoxyribonucleotides.
  • Nucleotides have three parts: a sugar, a phosphate, and a base.
  • Secondary DNA structure includes the double helix, phosphodiester bonds, hydrogen bond and base pairing, and antiparallel complementary strands.
  • The three-dimensional structure identified by Watson and Crick refers to B-DNA.

Special DNA and RNA Structures

  • Hairpin structures form in single strands of nucleotides when sequences on the same strand are inverted complements.
  • H-DNA is a three-stranded (triplex) structure formed when DNA unwinds and one strand pairs with double-stranded DNA from another part of the molecule.
  • DNA methylation involves adding methyl groups to nucleotide bases and affects the three-dimensional structure of DNA.

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