DNA Analysis in Forensics

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Questions and Answers

What is the correct expansion of the acronym DNA?

  • Deoxyribonucleic Acid (correct)
  • Denaturedribonucleic Acid
  • Deoxypolynitrogenous Acid
  • Desaturated Nuclear Adenosine

Which statement BEST describes the function of DNA?

  • An unique impression left behind by criminals at crime scenes
  • A unique subcellular component found in the ribosomes used for protein synthesis and identifying individuals
  • A molecule unique to each person containing instructions for building proteins (correct)
  • A protein unique to each person that affects the rate of chemical reactions

What is the correct sequence for DNA analysis steps after collecting a sample?

  • DNA extraction → DNA fingerprinting → DNA amplification
  • DNA fingerprinting → DNA extraction → DNA amplification
  • DNA amplification → DNA extraction → DNA fingerprinting
  • DNA extraction → DNA amplification → DNA fingerprinting (correct)

After receiving a DNA sample from a crime scene, what is the initial step a technician takes to extract the DNA?

<p>Use physical and chemical means to break down the cell and nuclear membranes (B)</p> Signup and view all the answers

Which of the following sequences accurately depicts the steps in Polymerase Chain Reaction (PCR)?

<p>Denaturing → Annealing → Extending (A)</p> Signup and view all the answers

A forensic lab obtains a severely degraded DNA sample. How does PCR assist in analyzing this sample?

<p>PCR can amplify even small fragments of DNA, making analysis possible. (A)</p> Signup and view all the answers

Why is DNA considered individual evidence in forensic science?

<p>It can be linked to a specific person (D)</p> Signup and view all the answers

Within the structure of DNA, what pairing relationship exists between nucleotide bases?

<p>A pairs with T, G pairs with C (C)</p> Signup and view all the answers

What is the main purpose of using CODIS in forensic science?

<p>To serve as a national DNA database (C)</p> Signup and view all the answers

Given a gel electrophoresis image showing DNA bands from a crime scene and three suspects, how would you determine a match?

<p>None of the suspects DNA complete matches the crime scene DNA. (D)</p> Signup and view all the answers

Flashcards

What does DNA stand for?

Deoxyribonucleic Acid: A molecule that carries genetic instructions for all living organisms.

What BEST describes DNA?

A molecule unique to each person containing instructions for building proteins.

Correct order of DNA analysis steps?

DNA extraction → DNA amplification → DNA fingerprinting.

First step to extract DNA?

Use physical and chemical means to break down the cell and nuclear membranes.

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Correct order of PCR steps?

Denaturing → Annealing → Extending

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How can PCR help analyze degraded DNA?

PCR can amplify even small fragments of DNA, making analysis possible.

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Why is DNA individual evidence?

It can be linked to a specific person.

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Relationship between nucleotide bases?

A pairs with T, G pairs with C.

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What is CODIS primarily used for?

To serve as a national DNA database.

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Based on the gel image, which statement is most accurate?

None of the suspects DNA complete matches the crime scene DNA.

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Study Notes

  • DNA stands for Deoxyribonucleic Acid.
  • DNA is a molecule unique to each person, containing instructions for building proteins.
  • The correct order of the steps of DNA analysis is DNA extraction → DNA amplification → DNA fingerprinting.
  • To extract DNA, laboratory technicians first use physical and chemical means to break down the cell and nuclear membranes after receiving the sample.
  • The correct order of the steps of PCR is Denaturing → Annealing → Extending.
  • PCR can help in analyzing a severely degraded DNA sample because it can amplify even small fragments of DNA, making analysis possible.
  • DNA is considered individual evidence in forensic science because it can be linked to a specific person.
  • In DNA structure, Adenine (A) pairs with Thymine (T), and Guanine (G) pairs with Cytosine (C).
  • CODIS is primarily used in forensic science to serve as a national DNA database.
  • Based on the gel image, suspect 2's DNA completely matches the crime scene DNA, confirming their involvement.

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