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Questions and Answers

What is the primary function of lysosomes in cellular processes?

  • Storage and release of calcium ions
  • Synthesis of ribonuclear proteins
  • Degradation of old or unnecessary cellular components (correct)
  • Synthesis of glycosaminoglycans
  • Which of the following accurately describes the structural characteristics of mitochondria?

  • Sausage-shaped and variable in number (correct)
  • Flat membrane-bound sacs with ribosomes
  • Unbound vesicles that store genetic material
  • Smooth tubules associated with lipid synthesis
  • During protein biosynthesis, which structure is primarily responsible for the modification and folding of proteins?

  • Smooth Endoplasmic Reticulum
  • Golgi Apparatus
  • Rough Endoplasmic Reticulum (correct)
  • Nucleus
  • What role do ribosomes play in the cell, particularly in protein biosynthesis?

    <p>They are sites for translation of mRNA into proteins</p> Signup and view all the answers

    What is the primary function of the smooth endoplasmic reticulum in the cell?

    <p>Lipid synthesis and detoxification</p> Signup and view all the answers

    What is the primary function of lysosomes in protein degradation?

    <p>To degrade damaged or useless proteins</p> Signup and view all the answers

    Which of the following correctly describes a characteristic of mitochondria?

    <p>They self-replicate and contain their own DNA.</p> Signup and view all the answers

    What is the role of ribosomes attached to the rough endoplasmic reticulum (rER)?

    <p>To synthesize proteins for secretion or membrane insertion.</p> Signup and view all the answers

    During the process of transcription, what is generated from a gene?

    <p>Pre-mRNA</p> Signup and view all the answers

    What is the main location of cholesterol and phospholipid synthesis within a cell?

    <p>Smooth endoplasmic reticulum (sER)</p> Signup and view all the answers

    Study Notes

    Cell Components and Functions

    • Mitochondria feature a smooth outer membrane and convoluted inner membrane, capable of self-replication; involved in cellular respiration.
    • The outer membrane contains porins and various enzymes, while the inner membrane is folded into cristae to increase surface area; the inner cavity, or matrix, holds mitochondrial DNA, ribosomes, and enzymes.
    • Energy production is facilitated through aerobic respiration in mitochondria, storing energy primarily as ATP.
    • Glycolysis occurs in the cytosol, producing pyruvate which migrates into mitochondria for further ATP synthesis; fatty acid metabolism is exclusively mitochondrial.

    Ribosomes

    • Composed of two rRNA subunits bound to proteins; can be either free in the cytoplasm or attached to the rough endoplasmic reticulum (rER).
    • Function to align mRNA with tRNA for the correct sequence of amino acids, catalyzing peptide bond formation.
    • Free ribosomes synthesize proteins for use within the cell, while those attached to rER produce proteins for export, lysosomes, and membrane proteins.

    Protein Biosynthesis

    • Begins with transcription, where pre-mRNA is synthesized as a complementary strand of DNA.
    • Post-transcription processing removes introns, transforming pre-mRNA into mature mRNA.
    • mRNA exits the nucleus and binds to ribosomes for translation, where it is read to produce proteins.

    Protein Degradation

    • Ubiquitin tags damaged proteins, which are then directed to proteasomes or degraded by lysosomal enzymes.

    Lipid Biosynthesis

    • Takes place in all cells, with fatty acids and triglycerides produced in the cytosol and cholesterol/phospholipids synthesized in the smooth endoplasmic reticulum (sER).
    • Lipid biosynthesis serves to replenish membranes, store energy, and maintain the continuity with the endoplasmic reticulum lumen.

    Nucleus

    • The largest organelle, surrounded by a nuclear envelope that regulates genetic material storage, replication, and controls cell differentiation.
    • Contains nucleolus, a site for rRNA synthesis and ribosome assembly; changes size based on cellular activity.
    • DNA is organized into chromatin, with euchromatin being active and heterochromatin being inactive.

    Endoplasmic Reticulum (ER)

    • Rough ER is characterized by attached ribosomes, playing a critical role in protein synthesis, modification, and folding.
    • Smooth ER is involved in lipid synthesis, detoxification, and calcium ion storage; detoxifies drugs via cytochrome P450 in the liver.

    Golgi Apparatus

    • Composed of membrane-bound cisternae that process and package proteins and lipids received from the rER.
    • Final assembly includes glycosylation of proteins and dispatching them to their destinations using SNARE proteins for targeting.

    Lysosomes

    • Contain hydrolytic enzymes active at low pH to degrade cellular debris and material taken in through endocytosis or phagocytosis.
    • Function in autophagy to remove damaged organelles and proteins.

    Peroxisomes

    • Membrane-bound organelles containing oxidases and catalase, important for detoxifying substances and β-oxidation of fatty acids.
    • Produce hydrogen peroxide to kill pathogens and help synthesize bile acids in the liver.

    Cell Membrane

    • Also known as plasma membrane; consists of an amphiphilic phospholipid bilayer embedded with proteins and cholesterol.
    • Functions to separate internal contents from the external environment, facilitate nutrient transport, and provide mechanical and chemical protection through the glycocalyx, which also aids in cell adhesion and signaling.

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