18 Questions
Why did McLeod and McCarty destroy different macromolecules?
To identify the genetic material.
In Hershey-Chase experiment, why did they use sulfur on protein and phosphate on DNA?
To label the protein and DNA for tracking purposes.
What is the chemical basis for base pairing in DNA?
Thymine bonds with Adenine due to hydrogen bonding.
Why is it important for purines to pair with pyrimidines in DNA?
To prevent DNA from becoming too thick or too thin.
What happens if purines bind with themselves according to Chargaff’s rule?
The DNA strand would be too thick.
Which form of DNA is described as being narrow, right spun, and the most common in nature?
B-DNA
What is the process where RNA copies DNA and converts it to mRNA?
Transcription
During Griffith's Experiment, why did the mouse die when exposed to the bacteria?
The bacteria underwent transformation from r-strain to s-strain
In McLeod and McCarty's Experiment, why did the mouse survive when exposed to the bacteria?
DNA was destroyed by DNase
What is one of the main roles of a bacteriophage in the Hershey-Chase Experiment?
Injecting DNA or RNA into the bacterial cell
What is the term for the process where bacteriophages cause the bacterial cell to burst and release more phages?
Lysis
In the Hershey-Chase Experiment, what was found in the pellet fraction after centrifugation?
$32P$-labeled phages
Which type of genetic material did the experiment involving tobacco mosaic virus demonstrate can be RNA?
DNA
What component of the reconstituted viruses was originally from virus b in the experiment?
Protein
Why is RNA more susceptible to degradation in cold temperatures compared to DNA?
RNA has one strand only
According to Chargaff's rules, what is the relationship between A and T, and G and C in DNA?
(A+G) = (C+T)
What does purines/pyrimidines ratio of 1.00 indicate regarding DNA composition?
Equal amounts of purines and pyrimidines
What risk does pairing too many purines or pyrimidines pose to DNA structure according to the text?
Makes DNA wider than 2 nm
Learn about the three groundbreaking experiments that led to the discovery of DNA as the genetic material by Griffith, McLeod, McCarty, and Hershey-Chase. Understand how DNA transformation, macromolecule destruction, and bacteriophage experiments played a crucial role in shaping our understanding of genetics.
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