10 Questions
What is the main limitation of the microscopic cell count method in bacterial enumeration?
Difficulty in distinguishing between viable and non-viable cells
What is the purpose of using a Petroff-Hausser counting chamber in the microscopic cell count method?
To count cells per unit area for calculating overall concentration
Why is it necessary to perform serial dilution on a bacterial broth culture before obtaining a countable number of viable bacteria?
To reduce the initial high cell density to a manageable range
What does a single, well-isolated colony on an agar plate represent in terms of bacterial replication?
The outcome of a single cell replicating at that location
What potential issue arises when motile cells are counted using the microscopic cell count method?
Double counting due to their movement across grid squares
What is the purpose of serial dilution in bacterial culture?
To decrease the concentration of the bacterial broth culture
What is the significance of subculturing/aliquoting after serial dilution?
To create multiple colonies from one viable bacterial cell
Why is the drop plate method preferred over the spread plate method for bacterial enumeration?
It uses less media
Which direct method of bacterial enumeration is best suited for samples with low microbial concentrations like wastewater?
Filtration method
In the pour-plate method, why is sterile medium added after pipetting the diluted culture onto a sterile plate?
To promote surface colony formation
Explore the various direct methods used for bacterial enumeration, including Microscopic cell count, CFU counts (Spread-plate, pour-plate, and drop-plate method), and Filtration method. Learn how these methods work in detail and their applications in microbiology.
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