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Questions and Answers
What is the composition of the dilution fluid used in the method described in the text?
What is the composition of the dilution fluid used in the method described in the text?
- Glacial acetic acid and gentian violet (correct)
- Glacial acetic acid and crystal violet
- Glacial acetic acid and safranin
- Glacial acetic acid and methyl blue
What is the purpose of the glass bead in the pipette used for accurate dilution?
What is the purpose of the glass bead in the pipette used for accurate dilution?
- To measure the volume of the fluid
- To ensure a consistent drop size (correct)
- To create a vacuum for suction
- To prevent the fluid from evaporating
What is the size of the small squares in the counting chamber used for counting cells?
What is the size of the small squares in the counting chamber used for counting cells?
- 1/16 sq.mm
- 1 sq.mm
- 1/20 sq.mm (correct)
- 1/32 sq.mm
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Study Notes
- The method involves diluting blood with a special fluid.
- The dilution fluid is made up of glacial acetic acid and gentian violet or methyl blue.
- A special pipette is used for accurate dilution.
- The pipette has a capillary tube with marks and a bulb containing a glass bead.
- A counting chamber is used for counting cells.
- The chamber has a ruled platform and is made of heavy glass.
- The central area of the platform is engraved with fine lines measuring 1 sq.mm.
- The central square is divided into 16 intermediate squares and each of these is further divided into 16 small squares.
- The small squares have sides whose length is 1/20 mm.
- A special plane coverslip of standard weight and thickness is used.
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