Diluting Blood and Counting Cells
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Diluting Blood and Counting Cells

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Questions and Answers

What is the composition of the dilution fluid used in the method described in the text?

  • Glacial acetic acid and gentian violet (correct)
  • Glacial acetic acid and crystal violet
  • Glacial acetic acid and safranin
  • Glacial acetic acid and methyl blue
  • What is the purpose of the glass bead in the pipette used for accurate dilution?

  • To measure the volume of the fluid
  • To ensure a consistent drop size (correct)
  • To create a vacuum for suction
  • To prevent the fluid from evaporating
  • What is the size of the small squares in the counting chamber used for counting cells?

  • 1/16 sq.mm
  • 1 sq.mm
  • 1/20 sq.mm (correct)
  • 1/32 sq.mm
  • Study Notes

    • The method involves diluting blood with a special fluid.
    • The dilution fluid is made up of glacial acetic acid and gentian violet or methyl blue.
    • A special pipette is used for accurate dilution.
    • The pipette has a capillary tube with marks and a bulb containing a glass bead.
    • A counting chamber is used for counting cells.
    • The chamber has a ruled platform and is made of heavy glass.
    • The central area of the platform is engraved with fine lines measuring 1 sq.mm.
    • The central square is divided into 16 intermediate squares and each of these is further divided into 16 small squares.
    • The small squares have sides whose length is 1/20 mm.
    • A special plane coverslip of standard weight and thickness is used.

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    Description

    "Cell Counting Quiz: Test Your Knowledge on Dilution Methods and Counting Chambers" - Do you know how to accurately dilute blood samples for cell counting? Can you identify the components of a counting chamber and its engraved platform? Test your understanding of the pipette and cover slip used in this process. Challenge yourself with our quiz and enhance your knowledge on cell counting techniques.

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