Developmental Biology - I Lab Experiments
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Questions and Answers

What is the total length of the sperm of Achatina fulica?

  • 1200 microns
  • 800 microns (correct)
  • 600 microns
  • 1000 microns
  • The ovum of Achatina fulica is large and opaque.

    False

    What is the primary color of the albumen gland in Achatina fulica before egg laying?

    lemon yellow

    The staining procedure involves using _____ stains to observe sperm and eggs.

    <p>Giemsa</p> Signup and view all the answers

    What stage follows the larval stage in the life cycle of Drosophila?

    <p>Pupal stage</p> Signup and view all the answers

    What is the characteristic structure of the sperm head in Achatina fulica?

    <p>Pear shaped</p> Signup and view all the answers

    Drosophila development processes are not related to human development processes.

    <p>False</p> Signup and view all the answers

    Match the following features with the corresponding organism:

    <p>Achatina fulica = Nuclei stained reddish purple Caenorhabditis elegans = Model organism for developmental biology Sperm structure = Elongated with a pointed anterior process Ovum characteristics = Tiny and creamy white</p> Signup and view all the answers

    What are the localized pre-adult tissues in the pupal stage called?

    <p>Imaginal discs</p> Signup and view all the answers

    Caenorhabditis elegans contains exactly 1000 somatic cells.

    <p>False</p> Signup and view all the answers

    Beetle life cycles include four stages: egg, larva, _____, and adult.

    <p>pupa</p> Signup and view all the answers

    What is the role of Caenorhabditis elegans in scientific research?

    <p>It is a model organism for molecular and developmental biology.</p> Signup and view all the answers

    Which of the following features is used to identify different phenotypes of flies?

    <p>Bristles and hairs on the body</p> Signup and view all the answers

    How does the pupa serve in insect development?

    <p>It allows for the development of adult structures from tissues.</p> Signup and view all the answers

    Match the species of Tribolium with their size:

    <p>T.brevicornis = B4 mm T.freemani = B5 mm T.audax = B3 mm or smaller T.confusum = B3 mm or smaller</p> Signup and view all the answers

    In some species, such as T.brevicornis, males are larger than females.

    <p>True</p> Signup and view all the answers

    What is the typical duration of embryogenesis for T.castaneum and T.confusum in ideal laboratory conditions?

    <p>3.5-5 days</p> Signup and view all the answers

    The larvae of T.castaneum and T.confusum are typically red in color.

    <p>False</p> Signup and view all the answers

    What unique structure do pupae of T.castaneum and T.confusum possess on their abdominal segments?

    <p>gin traps</p> Signup and view all the answers

    The typical life span of virgin adults in laboratory conditions for T.castaneum and T.confusum is ____ months.

    <p>7-11</p> Signup and view all the answers

    Match the following stages of the T.castaneum and T.confusum life cycle with their typical durations:

    <p>Egg = 3-4 weeks Larvae = 2-3 weeks Pupa = 5-6 days Adult = 1-6 months</p> Signup and view all the answers

    Which environmental factors influence the rate of development in flour beetles?

    <p>Temperature, humidity, and food</p> Signup and view all the answers

    Gemmules are produced by sponges to help them survive harsh environmental conditions.

    <p>True</p> Signup and view all the answers

    What happens to the fecundity of T.castaneum females after 3-4 months?

    <p>It drops</p> Signup and view all the answers

    What is the case called in which a pupa develops?

    <p>Puparium</p> Signup and view all the answers

    The pupa of butterflies is referred to as a cocoon.

    <p>False</p> Signup and view all the answers

    What do caterpillars primarily feed on after they hatch from their eggs?

    <p>Plants</p> Signup and view all the answers

    The caterpillar sheds its skin about _____ times as it grows.

    <p>4 or 5</p> Signup and view all the answers

    Match the following stages of butterfly development with their descriptions:

    <p>Egg = Laid on plants by the adult female Caterpillar = Feeds on plants and grows by molting Pupa = Develops inside a puparium or chrysalis Adult = Has long legs and can fly</p> Signup and view all the answers

    What is the primary function of the amphiblostula larva?

    <p>To give rise to a young sponge</p> Signup and view all the answers

    The planula larva has a mouth and can feed immediately after its formation.

    <p>False</p> Signup and view all the answers

    What type of larva is produced by the redia larva?

    <p>Cercaria larva</p> Signup and view all the answers

    The _____ larva is characterized by a flattened, ciliated body and is commonly found in cnidarians.

    <p>planula</p> Signup and view all the answers

    Which of the following describes the body composition of the amphiblostula larva?

    <p>A mix of flagellated and non-flagellated cells</p> Signup and view all the answers

    The cercaria larva is a free-living stage that follows the redia larva.

    <p>True</p> Signup and view all the answers

    How does the amphiblostula larva develop?

    <p>Through a process of inversion from the stomoblastula.</p> Signup and view all the answers

    Match each larva type with its characteristic feature:

    <p>Amphiblostula = Composed of flagellated and non-flagellated cells Planula = Free-swimming, flattened, ciliated form Redia = Gives rise to cercaria larvae Cercaria = Free living stage of liver fluke</p> Signup and view all the answers

    Which larval stage has a broad and crab-like unsegmented cephalothorax?

    <p>Megalopa larva</p> Signup and view all the answers

    The alima larva of Squilla hatches directly from the egg.

    <p>True</p> Signup and view all the answers

    What is the significance of the nauplius larva in crustacean development?

    <p>It is considered the ancestral form of crustaceans.</p> Signup and view all the answers

    All thoracic appendages in true crabs are _____ formed.

    <p>uniramous</p> Signup and view all the answers

    Match the larval forms with their descriptions:

    <p>Zoea larva = Characterized by multiple moults leading to megalopa Megalopa larva = Broad and crab-like with a median spine on carapace Alima larva = Modified zoea with glass-like transparency Nauplius larva = Ancestral larval form of crustaceans</p> Signup and view all the answers

    Which of the following is NOT a characteristic of the alima larva?

    <p>Broad cephalothorax</p> Signup and view all the answers

    According to the biogenetic law proposed by Haeckel, ontogeny recapitulates morphology.

    <p>False</p> Signup and view all the answers

    Identify the larval stage in crabs that is characterized by large, stalked eyes.

    <p>Megalopa larva</p> Signup and view all the answers

    Study Notes

    Developmental Biology - I

    • PSMAZOP102 is the course code.

    Experiments

    • Experiment 1 (1-4): Observe sperm and ova in ovotestes of Achatina fulica (garden snail) using suitable staining techniques.
    • Experiment 2 (5-6): Observe the development of Caenorhabditis elegans.
    • Experiment 3 (7-11): Culture Drosophila to study its life cycle.
    • Experiment 4 (12): Observe stages of Tribolium or Sitophilus to understand indirect development in animals.
    • Experiment 5 (13): Study Gemmule in Sponge.
    • Experiment 6 (14-25): Study larvae of non-chordates including Porifera, Cnidaria, Platyhelminthes, Annelida, Mollusca, and Echinodermata & Hemichordata.
    • Experiment 7 (26-30): Temporary mounting of crustacean life forms (Nauplius, Zoea, Mysis, Megalopa, and Alima).
    • Experiment 8 (31-33): Study the life cycle of insects (Lepisma, Cockroach, Butterfly/Moth).

    Achatina fulica (Garden Snail)

    • The snails used for the study originated from natural and breeding environments.
    • Achatina fulica is a protandrous hermaphrodite, facilitating early detection of male gametes.
    • Environmental factors (humidity, temperature, photoperiod) and food quality influence snail breeding.
    • Low humidity causes estivation & growth disorders.
    • High temperature negatively impacts growth and reproduction.

    Genital System of Achatina fulica

    • Ovotestis is a true multilobate structure, divisible into apical portion, ovisperm vesicle, and basal portion ending in the carrefour.
    • Spermatozoa are stored in the ovisperm vesicle year-round.
    • The spermoviduct is long and has a narrow sulcus, incompletely separated from the broader uterus.
    • The vas deferens connects to the penis and the uterus continues as the oviduct, opening into the vagina.
    • The penis & the vagina open into the genital atrium.

    Stages of Gametogenesis (Achatina)

    • Four stages of gametogenesis in the male and female, based on length classes and the original environment.

    Stage 1 of Maturation

    • Phase of sexual rest with reduced gonads (acini).

    Stage 2 of Maturation

    • Gonia multiply by mitosis.
    • Poor development of the gonadal volume in the connective tissue.

    Stage 3 of Maturation

    • The gonadal cells increase in size through vitellogenesis.
    • Connective tissue starts to disappear around the gonadal cells.

    Stage 4 of Maturity

    • Acini are fully filled with mature oocytes.
    • Nucleolus and a distinct nucleus are visible in the oocytes.
    • Sperms are abundant in the follicles.

    Caenorhabditis elegans

    • A small, unsegmented, vermiform, free-living nematode.
    • Simple and precisely structured.
    • A crucial model organism in developmental biology.
    • Genetic lineage tracing in a transparent cuticle
    • Entire genome is sequenced.
    • 1 mm long - a model organism

    Drosophila melanogaster

    • Fruit flies, thrive on fruits, especially ripe bananas.
    • Sexually dimorphism is observed (male smaller than female, different shapes & colours in the abdomen).
    • Use jars with cotton wool and fruit for culturing.
    • Various stages of Drosophila (egg, larva, pupa, adult).

    Tribolium/Sitophilus

    • Beetles, with distinct life cycles (egg, larva, pupa, adult).
    • Adults are sexually dimorphic.
    • Differences in shape and size are observed.
    • Certain species show differing size ratios between males and females.

    Gemmule (Sponge)

    • Gemmules are hardened masses of cells functioning as dormant buds in sponges.

    Larvae of Non-Chordates

    • Study of various larval forms of marine invertebrates: Amphiblastula, Planula, Redia, Cercaria, Trochophore, Glochidium, Veliger, Auricularia, Echinopluteus, Ophiopluteus, Tornaria.
    • Different larval forms characterized by morphological features like symmetry, appendage arrangements, feeding mechanisms, and presence of cilia or other movement capabilities.

    Temporary Mounting of Crustacean Life Forms

    • Techniques for fixing and preserving crustacean life forms.
    • Methods involve staining with alcohol-based eosin.

    Life Cycle of Insects

    • Classification of insect metamorphosis (No-metamorphosis, Gradual, Complete, Incomplete).

    Methods, Preservation

    • Fixing and preserving the plankton sample in formalin or alcohol for a few days.

    Additional Notes

    • Additional notes are detailed in the document for each of the listed topics, including detailed descriptions, examples, relevant diagrams, and specific steps or procedures involved with each topic.

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    Description

    This quiz covers various experiments in Developmental Biology - I, specifically focusing on the observation and study of sperm, ova, and the life cycles of different organisms such as Achatina fulica, C. elegans, and Drosophila. Students will explore indirect development and larvae of non-chordates, enhancing their understanding of developmental processes in different species.

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