COPY: Detection of Diseases: COVID-19 Testing

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Questions and Answers

What is the primary purpose of the ELISA technique described?

  • To amplify nucleic acids for detection
  • To separate proteins based on their charge
  • To detect specific viral antigens in a patient sample (correct)
  • To determine the size of proteins in a sample

Which step in the Western blotting process is crucial for the specific detection of the target protein?

  • Exposing the membrane to a specific antibody (correct)
  • Washing the membrane with buffer solution
  • Transferring proteins to a membrane
  • Separation of the proteins using gel electrophoresis

What mechanism does PCR utilize to detect nucleic acids?

  • Hybridization of antibodies with proteins
  • Electrophoresis for size separation
  • Amplification of specific DNA sequences (correct)
  • Colorimetric detection of proteins

What advantage do rapid tests provide in the context of detecting viral infections?

<p>They provide immediate results without laboratory equipment. (B)</p> Signup and view all the answers

In an ELISA test, what happens after the addition of enzyme-bound secondary antibodies?

<p>A color change occurs due to the substrate addition. (D)</p> Signup and view all the answers

What is confirmed by the presence of the red line at the Control Line in an antigen rapid test?

<p>The test is valid and worked properly. (C)</p> Signup and view all the answers

What happens if the red line at the Control Line does not appear during an antibody test?

<p>The entire test is deemed invalid. (B)</p> Signup and view all the answers

In the process of RT-PCR, what is the first step after RNA extraction?

<p>Reverse transcription. (A)</p> Signup and view all the answers

How does a TaqMan probe function in real-time PCR?

<p>It releases fluorescent signals upon degradation during PCR. (B)</p> Signup and view all the answers

What characterizes rapid tests like antigen tests?

<p>Results can be seen with the naked eye within 15 minutes. (D)</p> Signup and view all the answers

During the RT-PCR process, what role does the Taq polymerase play in the assay?

<p>It ensures the degradation of the TaqMan probe. (B)</p> Signup and view all the answers

What is the purpose of primer annealing during PCR amplification?

<p>To allow primers to bind to the target DNA. (B)</p> Signup and view all the answers

In the context of nucleic acid detection, what is cDNA?

<p>Complementary DNA synthesized from RNA. (C)</p> Signup and view all the answers

What does the control line in a test kit indicate?

<p>The test kit has not expired (C)</p> Signup and view all the answers

Which component in the test kits binds to the Covid-19 IgG or IgM?

<p>Mouse Anti-Human IgM or IgG antibody (A)</p> Signup and view all the answers

What is the main advantage of Antigen Rapid Tests for COVID-19 detection?

<p>They simplify the testing process for home use (A)</p> Signup and view all the answers

What type of interaction do antigen rapid tests primarily rely on?

<p>Immunological antigen-antibody interaction (D)</p> Signup and view all the answers

Which component is primarily responsible for maintaining the stability of the sample in the test kit?

<p>Buffer solution (C)</p> Signup and view all the answers

What is the role of the Gold-Rabbit IgG conjugate in the test kits?

<p>To move along the liquid and bind to anti-rabbit IgG (A)</p> Signup and view all the answers

In the test process, what happens if the patient's sample contains the virus?

<p>Red lines will appear on the corresponding M or G line (C)</p> Signup and view all the answers

Which of the following does not contribute to the action of antigen rapid tests?

<p>Complex laboratory analysis (D)</p> Signup and view all the answers

Which shape of bacteria is classified as 'cocci'?

<p>Spherical (D)</p> Signup and view all the answers

What is a common method by which pathogenic bacteria cause disease?

<p>Releasing toxins into the bloodstream (C)</p> Signup and view all the answers

Which of the following is NOT a use of bacteria?

<p>Causing food poisoning (C)</p> Signup and view all the answers

What characteristic do viruses lack compared to living organisms?

<p>Ability to reproduce on their own (B)</p> Signup and view all the answers

What is the role of bacteria in human health?

<p>Digesting food and making vitamins (B)</p> Signup and view all the answers

Which of the following diseases is commonly caused by bacteria?

<p>Cholera (A)</p> Signup and view all the answers

What component do bacteria have that makes up their cell wall?

<p>Peptidoglycan (A)</p> Signup and view all the answers

What process describes how nitrogen-fixing bacteria support plant life?

<p>Changing nitrogen gas into ammonia (B)</p> Signup and view all the answers

What is a significant consequence of antibiotic use in bacteria?

<p>Bacteria develop resistance to antibiotics (D)</p> Signup and view all the answers

Which mechanism is NOT utilized by antivirals to combat viral infections?

<p>Killing the virus permanently (A)</p> Signup and view all the answers

What is the primary role of vaccines in infectious disease prevention?

<p>Stimulate the immune system to produce antibodies (D)</p> Signup and view all the answers

Which of the following describes a true zone of inhibition in antibiotic testing?

<p>A clear, large area with minimal bacterial growth (C)</p> Signup and view all the answers

Which antiviral drug is specifically used to treat the flu virus?

<p>Zanamivir (C), Oseltamivir (D)</p> Signup and view all the answers

What is a common feature of both antibiotic and antiviral resistance?

<p>Resistance can lead to ineffective treatments (D)</p> Signup and view all the answers

What does it mean when an antiviral only shortens symptoms by 1-2 days?

<p>It does not provide a long-term solution to the infection (A)</p> Signup and view all the answers

How do vaccines allow the body to respond effectively to future infections?

<p>By tricking the immune system to recognize pathogens (A)</p> Signup and view all the answers

What is the main outcome of the lytic cycle in viral replication?

<p>The host cell is killed and bursts, releasing new viruses. (C)</p> Signup and view all the answers

Which process differentiates the lysogenic cycle from the lytic cycle?

<p>The viral nucleic acid integrates into the host's chromosome. (C)</p> Signup and view all the answers

What type of genetic material do all viruses possess at their core?

<p>Nucleic acids (DNA or RNA) (C)</p> Signup and view all the answers

How do antibiotics function in the treatment of infections?

<p>They kill only bacteria and do not affect viruses. (C)</p> Signup and view all the answers

Which of the following diseases is caused by a virus?

<p>Chickenpox (B)</p> Signup and view all the answers

Which statement about the viral structure is true?

<p>All viruses possess a protein coat called a capsid. (C)</p> Signup and view all the answers

What is the primary role of antivirals in treating infections?

<p>To specifically inhibit the replication of viruses. (C)</p> Signup and view all the answers

Which of the following represents a characteristic of the lysogenic cycle?

<p>Formation of a provirus that replicates with the host cell. (D)</p> Signup and view all the answers

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