Podcast
Questions and Answers
Which technique is commonly used to detect known mutations in inherited diseases?
Which technique is commonly used to detect known mutations in inherited diseases?
- PCR and direct sequencing
- PCR and allele-specific probes (correct)
- Direct sequencing
- Multiple approaches
Why is direct sequencing not widely used for the research of known mutations?
Why is direct sequencing not widely used for the research of known mutations?
- It is time-consuming
- It is not effective
- It is expensive (correct)
- It is not applicable in all laboratories
What is the main characteristic of the mutations detected using these techniques?
What is the main characteristic of the mutations detected using these techniques?
- They are universal
- They have already been characterized (correct)
- They are unknown
- They are caused by multiple diseases
What is the limitation of the techniques used to detect known mutations?
What is the limitation of the techniques used to detect known mutations?
Why do laboratories with limited resources and expertise not use direct sequencing?
Why do laboratories with limited resources and expertise not use direct sequencing?
Which type of targeting was discussed in the previous lecture?
Which type of targeting was discussed in the previous lecture?
What is the purpose of transcriptional targeting in gene therapy?
What is the purpose of transcriptional targeting in gene therapy?
What is the role of the promoter in transcriptional targeting?
What is the role of the promoter in transcriptional targeting?
What is the main difference between transductional targeting and transcriptional targeting?
What is the main difference between transductional targeting and transcriptional targeting?
How can transcriptional targeting achieve tissue-specific gene expression?
How can transcriptional targeting achieve tissue-specific gene expression?
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