Detecting Known Mutations in Inherited Diseases
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Questions and Answers

Which technique is commonly used to detect known mutations in inherited diseases?

  • PCR and direct sequencing
  • PCR and allele-specific probes (correct)
  • Direct sequencing
  • Multiple approaches
  • Why is direct sequencing not widely used for the research of known mutations?

  • It is time-consuming
  • It is not effective
  • It is expensive (correct)
  • It is not applicable in all laboratories
  • What is the main characteristic of the mutations detected using these techniques?

  • They are universal
  • They have already been characterized (correct)
  • They are unknown
  • They are caused by multiple diseases
  • What is the limitation of the techniques used to detect known mutations?

    <p>They cannot identify all genetic diseases</p> Signup and view all the answers

    Why do laboratories with limited resources and expertise not use direct sequencing?

    <p>It is expensive</p> Signup and view all the answers

    Which type of targeting was discussed in the previous lecture?

    <p>Transductional targeting</p> Signup and view all the answers

    What is the purpose of transcriptional targeting in gene therapy?

    <p>To obtain tissue or cell specific gene expression</p> Signup and view all the answers

    What is the role of the promoter in transcriptional targeting?

    <p>To control gene expression in specific tissues</p> Signup and view all the answers

    What is the main difference between transductional targeting and transcriptional targeting?

    <p>Transcriptional targeting allows vector entry into any cell type</p> Signup and view all the answers

    How can transcriptional targeting achieve tissue-specific gene expression?

    <p>By using a tissue-specific promoter in the vector</p> Signup and view all the answers

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