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Questions and Answers
Which technique is commonly used to detect known mutations in inherited diseases?
Which technique is commonly used to detect known mutations in inherited diseases?
- PCR and direct sequencing
- PCR and allele-specific probes (correct)
- Direct sequencing
- Multiple approaches
Why is direct sequencing not widely used for the research of known mutations?
Why is direct sequencing not widely used for the research of known mutations?
- It is time-consuming
- It is not effective
- It is expensive (correct)
- It is not applicable in all laboratories
What is the main characteristic of the mutations detected using these techniques?
What is the main characteristic of the mutations detected using these techniques?
- They are universal
- They have already been characterized (correct)
- They are unknown
- They are caused by multiple diseases
What is the limitation of the techniques used to detect known mutations?
What is the limitation of the techniques used to detect known mutations?
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Why do laboratories with limited resources and expertise not use direct sequencing?
Why do laboratories with limited resources and expertise not use direct sequencing?
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Which type of targeting was discussed in the previous lecture?
Which type of targeting was discussed in the previous lecture?
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What is the purpose of transcriptional targeting in gene therapy?
What is the purpose of transcriptional targeting in gene therapy?
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What is the role of the promoter in transcriptional targeting?
What is the role of the promoter in transcriptional targeting?
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What is the main difference between transductional targeting and transcriptional targeting?
What is the main difference between transductional targeting and transcriptional targeting?
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How can transcriptional targeting achieve tissue-specific gene expression?
How can transcriptional targeting achieve tissue-specific gene expression?
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