Density-Gradient Centrifugation Quiz
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Questions and Answers

What method is used to further separate biological particles of similar size but differing density?

Density-gradient centrifugation

What are the two methods that can be used for ultracentrifugation with preformed or self-establishing density gradients?

Rate separation or equilibrium methods

What is caesium chloride widely used for in preparative ultracentrifugation?

Banding of DNA and the isolation of plasmids, nucleoproteins and viruses

What are sodium bromide and sodium iodide employed for in the ultracentrifugation process?

<p>Fractionation of lipoproteins and the banding of DNA or RNA molecules, respectively</p> Signup and view all the answers

What are some examples of gradient materials available for the separation of whole cells and subcellular particles?

<p>Percoll, F</p> Signup and view all the answers

What is the method of choice for further separating biological particles of similar size but differing density?

<p>Ultracentrifugation with preformed or self-establishing density gradients</p> Signup and view all the answers

What are the two methods that can be used for density-gradient centrifugation?

<p>Rate separation or equilibrium methods</p> Signup and view all the answers

Which gradient material is widely used for the banding of DNA and the isolation of plasmids, nucleoproteins, and viruses?

<p>Caesium chloride</p> Signup and view all the answers

What are sodium bromide and sodium iodide employed for in density-gradient centrifugation?

<p>Fractionation of lipoproteins and the banding of DNA or RNA molecules, respectively</p> Signup and view all the answers

Name a range of gradient material offered by various companies for the separation of whole cells and subcellular particles.

<p>Percoll, F</p> Signup and view all the answers

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