Questions and Answers
Match the authors with their publication title:
Zarlenga, D.S. = The Identification of Haemonchus Species and Diagnosis of Haemonchosis Högberg, N. = Nematode Parasitism Affects Lying Time and Overall Activity Patterns in Lambs Following Pasture Exposure around Weaning Höglund, J. = Signs of Multiple Anthelmintic Resistance in Sheep Gastrointestinal Nematodes in Sweden Daş, G. = Egg Production Dynamics and Fecundity of Heterakis gallinarum Residing in Different Caecal Environments of Chickens Induced by Fibre-Rich Diets
Match the journals with their publication titles:
Front. Vet. Sci. = Comparison of Four Diagnostic Methods for Detection and Relative Quantification of Haemonchus contortus Eggs in Feces Samples Vet. Parasitol. = The Effect of Weaning Age on Animal Performance in Lambs Exposed to Naturally Acquired Nematode Infections Parasitol. Res. = Molecular Detection of Two Major Gastrointestinal Parasite Genera in Cattle Using a Novel Droplet Digital PCR Approach Vet. Parasitol. Reg. Stud. Rep. = Signs of Multiple Anthelmintic Resistance in Sheep Gastrointestinal Nematodes in Sweden
Match the publication years with their corresponding publication titles:
2018 = Comparison of Four Diagnostic Methods for Detection and Relative Quantification of Haemonchus contortus Eggs in Feces Samples 2016 = The Identification of Haemonchus Species and Diagnosis of Haemonchosis 2021 = Nematode Parasitism Affects Lying Time and Overall Activity Patterns in Lambs Following Pasture Exposure around Weaning 2014 = Egg Production Dynamics and Fecundity of Heterakis gallinarum Residing in Different Caecal Environments of Chickens Induced by Fibre-Rich Diets
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Study Notes
ddPCR Assay for Detecting Parasitic Infections
- A novel ddPCR assay was developed to detect and quantify parasitic infections, with a significantly higher dilution effect than conventional PCR and qPCR.
- The assay's performance is not affected by PCR inhibitors, and it has the potential to perform DNA sequencing to confirm a positive result.
Tracking Treatment Response in Bovine Theileriosis
- The ddPCR assay was used to track the treatment response in clinical cases of bovine theileriosis, which is a critical feature.
- The study's findings demonstrated the ability of this novel tool to track treatment response.
Detecting Toxoplasma gondii in Meat Samples
- A ddPCR assay was developed to detect and quantify Toxoplasma gondii (T. gondii) DNA in meat samples from intermediate hosts.
- The assay showed high sensitivity (97.5%) and specificity (100%) and resulted in higher positivity rates in meat samples (7.6% vs. 1.2% in qPCR).
- The detection limit was 8 genomic copies/µL, making it essential for T. gondii detection in meat samples due to the non-homogeneous distribution of tissue cysts and the small sample size.
Detecting Toxoplasma gondii in Edible Fishes
- A dPCR platform was used to investigate the hypothesis that edible fishes can harbor and transmit T. gondii to marine organisms and human consumers.
- The study showed that fish specimens from 12 different fish species were contaminated with T. gondii DNA.
Detecting Gastrointestinal Nematodes in Sheep
- A ddPCR assay was developed to detect and quantify the DNA copies of the three major strongylid genera infecting sheep.
- The assay was used to determine the occurrence of gastrointestinal nematodes in sheep under natural grazing conditions.
- The assay was also used to evaluate the efficacy of different anthelmintics and to complement FECRT and other routine diagnostic methods.
Detecting Gastrointestinal Nematodes in Cattle
- A ddPCR assay was developed to discriminate gastrointestinal nematodes frequently identified in mixed infections in cattle.
- The assay targeted Cooperia spp. and Ostertagia spp. and produced promising results in terms of fractional abundance and LOD for each parasite genus in the presence of the other.
Advantages of ddPCR Assays
- ddPCR assays developed so far presented high sensitivity and specificity, as well as low levels of detection, comparable to or even higher than qPCR and NGS platforms.
- ddPCR assays can accurately detect low parasitic loads in clinical samples, facilitating early diagnosis and treatment initiation at individual and flock levels, even in mixed infections.
- ddPCR assays can be used for absolute quantification of the parasitic burden, monitoring disease progression, and treatment outcomes.
- ddPCR assays have the potential to be used for the discovery of new molecular markers to identify resistance to parasiticides and new targets for drug development.
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