Cytoplasmic Membrane Structure and Transport

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Questions and Answers

What is the primary role of fimbriae in bacterial cells?

  • Energy production through ATP synthesis
  • Facilitating bacterial conjugation
  • Biomineralization for environmental stability
  • Adhesion to surfaces and cells (correct)

What distinguishes pili from fimbriae in terms of their structural characteristics?

  • Pili are involved in adhesion but not in motility
  • Fimbriae are shorter and narrower than pili (correct)
  • Pili are present in both Gram-positive and Gram-negative bacteria
  • Fimbriae are longer and broader than pili

In which type of bacteria would you primarily find pili?

  • Only Gram-negative bacteria (correct)
  • Archaea and Gram-negative bacteria
  • Both Gram-positive and Gram-negative
  • Only Gram-positive bacteria

Which of the following accurately describes the role of polyphosphate in bacterial cells?

<p>Aiding in ATP synthesis and phosphate storage (B)</p> Signup and view all the answers

Which statement regarding biofilms is correct?

<p>Biofilms consist of communities of bacteria encased in a protective layer (A)</p> Signup and view all the answers

What is the primary function of the periplasm in Gram-negative bacteria?

<p>To contain enzymes and transport proteins for substance transport (D)</p> Signup and view all the answers

Which structural component is responsible for the retention of crystal violet in Gram-positive bacteria?

<p>Peptidoglycan (A)</p> Signup and view all the answers

What role do porins play in the outer membrane of Gram-negative bacteria?

<p>They facilitate the entry of small molecules and nutrients (A)</p> Signup and view all the answers

What is the main component that distinguishes the cell walls of archaea from those of bacteria?

<p>Composition of pseudomurein instead of peptidoglycan (A)</p> Signup and view all the answers

During the Gram staining process, what is the function of iodine treatment?

<p>To enhance crystal violet retention (C)</p> Signup and view all the answers

What kind of ATP-binding transport proteins are part of the ABC transport system?

<p>ATP-hydrolyzing proteins (A)</p> Signup and view all the answers

Pili are found in which type of bacteria, and what is their primary function?

<p>Gram-negative, for adhering to surfaces (D)</p> Signup and view all the answers

What is the composition of peptidoglycan found in bacterial cell walls?

<p>Alternating units of N-acetylglucosamine and N-acetylmuramic acid (B)</p> Signup and view all the answers

What role do polyphosphate granules primarily serve in bacteria?

<p>Energy and phosphate storage (D)</p> Signup and view all the answers

What structure in flagella comprises the rotor and stator components?

<p>Motor (A)</p> Signup and view all the answers

How do polar flagellated bacteria change direction during movement?

<p>By reversing flagella rotation (A)</p> Signup and view all the answers

Which of the following components is NOT part of the flagellar structure?

<p>Capsule (A)</p> Signup and view all the answers

What does the term 'chemotaxis' refer to in bacteria?

<p>Movement towards or away from chemical stimuli (B)</p> Signup and view all the answers

What is the width range of archaeal flagella?

<p>10-13 nm (D)</p> Signup and view all the answers

In the Capillary Tube Assay for measuring chemotaxis, what happens when an attractant is present?

<p>Microorganisms swarm towards the chemical (A)</p> Signup and view all the answers

Which statement about flagellar synthesis is correct?

<p>Assembly begins with MS and C rings (A)</p> Signup and view all the answers

What is the primary motivation behind the oxidation of granule sulfur in bacteria?

<p>Due to limited supply of reduced sulfur (C)</p> Signup and view all the answers

What type of bacterial flagellation is characterized by flagella distributed over the entire surface of the cell?

<p>Peritrichous flagellation (B)</p> Signup and view all the answers

What characterizes the cytoplasmic membrane's structure?

<p>It contains unique lipids such as crenarchaeol. (B)</p> Signup and view all the answers

Which function does the cytoplasmic membrane NOT serve?

<p>Providing energy directly. (C)</p> Signup and view all the answers

How do hopanoids influence bacterial membranes?

<p>They stabilize the structure. (C)</p> Signup and view all the answers

What role do aquaporins play in the cytoplasmic membrane?

<p>They facilitate rapid transport of water. (A)</p> Signup and view all the answers

What structural feature of the cytoplasmic membrane contributes to its fluidity?

<p>The lipid nature of the membrane. (C)</p> Signup and view all the answers

What stereochemical difference exists between the glycosidic bonds of pseudomurein compared to peptidoglycan?

<p>β(1→3) in pseudomurein and β(1→4) in peptidoglycan (C)</p> Signup and view all the answers

Which of the following correctly describes the functions of polyphosphate in cells?

<p>Precursor for ATP production and phosphate storage (C)</p> Signup and view all the answers

What type of proteins are integral to the cytoplasmic membrane?

<p>They span the membrane with both hydrophobic and hydrophilic regions. (A)</p> Signup and view all the answers

What is a primary function of the S-layer in archaea?

<p>Retaining proteins and serving as a selective sieve (D)</p> Signup and view all the answers

Which statement about membrane permeability is accurate?

<p>Only small non-polar molecules can freely pass through. (A)</p> Signup and view all the answers

How does the glycocalyx contribute to bacterial survival?

<p>By assisting in cell binding and protecting against desiccation (C)</p> Signup and view all the answers

What distinguishes transport proteins in the cytoplasmic membrane?

<p>They aid in both active and passive transport mechanisms. (B)</p> Signup and view all the answers

What type of lipids are commonly found in the cytoplasmic membrane?

<p>Fatty acids ranging from 14 to 20 carbon atoms. (C)</p> Signup and view all the answers

Which statement about sulfur in cells is accurate?

<p>Sulfur is essential for energy metabolism and CO2 fixation (C)</p> Signup and view all the answers

What role do carbonate minerals play in prokaryotes?

<p>They stabilize cellular environments through biomineralization (D)</p> Signup and view all the answers

Which of the following correctly identifies the source of sulfur used by cells?

<p>Reduced sulfur sources within cells (C)</p> Signup and view all the answers

What distinguishes fimbriae from pili in prokaryotic cells?

<p>Fimbriae are found in both Gram-positive and Gram-negative bacteria (A)</p> Signup and view all the answers

What is a pivotal function of polyphosphate granules within cells?

<p>Serving as both energy and phosphate storage (B)</p> Signup and view all the answers

What is a characteristic feature of Staphylococcus species in relation to biofilms?

<p>They form biofilms through slime layer development (B)</p> Signup and view all the answers

Flashcards

Cytoplasmic Membrane

A biological membrane that encloses the cytoplasm of a cell, separating it from the external environment. It acts as a barrier, controlling what enters and exits the cell.

Cytoplasm

The fluid, gel-like substance within the cell membrane, containing all the organelles and other cellular components.

Phospholipids

Specialized fatty molecules that form the structural basis of the cytoplasmic membrane. They have a unique structure consisting of a hydrophilic head and a hydrophobic tail.

Selective Permeability

A property of the cytoplasmic membrane that allows it to control the passage of molecules in and out of the cell. The membrane lets some substances pass through easily, while others are blocked.

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Membrane Proteins

Proteins embedded within the cytoplasmic membrane, crucial for various cellular processes such as transport, signaling, and adhesion.

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Transport Proteins

Membrane proteins that facilitate the movement of specific molecules across the membrane.

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Channel Proteins

A type of transport protein that creates a hydrophilic channel allowing water and small polar molecules to pass through the membrane.

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Hopanoids

Specialized lipids that increase the stability of bacterial membranes. Think of them as structural reinforcements.

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Crenarchaeol

Unique lipids found in hyperthermophilic Archaea. They contain cyclopentyl and cyclohexyl rings, making them more rigid.

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Membrane Function

The membrane acts as a barrier, maintaining a stable internal environment despite external fluctuations. It prevents unrestricted diffusion of substances, ensuring a controlled environment for cell processes.

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What is the periplasm?

The gel-like space between the inner and outer membranes of Gram-negative bacteria, containing enzymes and proteins.

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What are porins?

Specialized proteins in the outer membrane of Gram-negative bacteria that allow the passage of small molecules and nutrients into the periplasm; they can be specific or non-specific.

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Explain the ABC transport system.

A type of active transport system found in bacteria, consisting of periplasmic binding proteins, membrane transporters, and ATP-hydrolyzing proteins to efficiently transport substances across the membrane.

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What is peptidoglycan?

A major component of bacterial cell walls, made of alternating units of N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM). It provides structural integrity, rigidity, and shape to bacterial cells.

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Describe the Gram staining process.

A staining technique that differentiates bacteria based on their cell wall structure. Gram-positive bacteria retain the crystal violet stain, appearing purple, while Gram-negative bacteria lose the stain and take up the counterstain, appearing pink or red.

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What is pseudomurein?

A type of cell wall found in some Archaea, containing N-acetylglucosamine and N-acetyltalosaminuronic acid instead of N-acetylmuramic acid. It provides structural support similar to peptidoglycan.

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What are pili?

Hair-like appendages found only in Gram-negative bacteria, typically 1-4 per cell. They are involved in conjugation processes, where genetic material is transferred between bacteria.

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What are fimbriae?

Short, bristle-like appendages found in both Gram-positive and Gram-negative bacteria. They help bacteria adhere to surfaces, form biofilms, and sometimes contribute to virulence.

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What is a biofilm?

A protective layer surrounding bacterial cells that enhances survival, resistance, and biofilm formation.

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What is swimming motility?

A complex process where bacteria use flagella to move through their environment.

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What are flagella?

These structures, 15-20 nm in size, enable bacteria to move through their environment by 'swimming'.

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S-Layer

A paracrystalline layer of interlocking proteins or glycoproteins that acts as a selective sieve, retaining proteins close to the cell surface and providing structural support.

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Glycocalyx

A thin, deformed layer surrounding bacterial cell walls, composed of polysaccharides and glycoproteins. It helps with cell binding, nutrient trapping, surface adhesion, and protection against desiccation.

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Fimbriae

Short, bristle-like appendages found on bacterial surfaces, involved in adhesion to surfaces and other cells. They are essential for biofilm formation and colonization.

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Pseudomurein

A unique cell wall polymer found in Archaea, differing from peptidoglycan in glycosidic bond structure and stereochemistry. It provides strength and rigidity to the cell wall.

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Polyphosphate

A type of polysaccharide found in bacteria and some eukaryotes, serving as an energy storage and phosphate reservoir. It can be converted to ATP, providing energy for various cellular processes.

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Chemolithotrophy

The process by which prokaryotes utilize sulfur for energy metabolism, often in environments lacking oxygen.

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Autotrophy

The process by which organisms use carbon dioxide as their primary carbon source to produce organic compounds. It is often coupled with chemolithotrophy in sulfur-oxidizing bacteria.

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Biomineralization

A process involving the formation of mineral structures, often catalyzed by prokaryotes. It plays a significant role in stabilizing cellular environments and shaping habitats.

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Benstonite

A type of mineral, often formed by prokaryotes, that is important for maintaining cellular environments and providing a stable habitat.

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Sulfur Oxidation

A process by which bacteria use sulfur as an electron donor for energy generation. It is a crucial step in the sulfur cycle, playing a significant role in ecosystems.

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Polyphosphate Function

Polyphosphate granules store energy and phosphate, releasing ATP when broken down.

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Sulfur Metabolism

Sulfur is primarily sourced from reduced sulfur. When limited, the cell oxidizes sulfur granules to sulfate.

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Carbonate Mineral Formation

Filamentous cyanobacteria like Gleomargarita form carbonate minerals like benstonite.

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Peritrichous Flagellation

Flagella are helical structures found over the entire surface of the cell, propelling bacterial movement.

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Flagellar Structure

Flagella are composed of a filament, hook, and motor, powered by the proton motive force.

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Flagellar Movement

Flagella act like a motor, rotating to propel the cell forward or backward.

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Chemotaxis

Bacteria move towards attractants and away from repellents using flagellar motion.

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Peritrichous Flagellar Movement

Peritrichous flagellated bacteria move forward in runs and tumble when they stop and jiggle.

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Polar Flagellar Movement

Polar flagellated bacteria can reverse flagellar rotation to change movement direction.

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Archaeal Flagella

Archaeal flagella are composed of different flagellin proteins and powered directly by ATP.

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Study Notes

Cytoplasmic Membrane Structure and Transport

  • Cytoplasmic membrane acts as a barrier, controlling what enters and leaves the cytoplasm.
  • Composed of a phospholipid bilayer, about 8-10 nanometers wide.
  • Contains fatty acids (14-20 carbons), giving it structure.
  • Fluid-like, not rigid, due to its lipid nature.
  • Hopanoids (sterol-like compounds) stabilize bacterial membranes.

Membrane Proteins

  • Proteins span the membrane, with hydrophobic parts embedded and hydrophilic parts interacting with the surroundings.
  • Integral proteins are firmly embedded; peripheral proteins are loosely associated.
  • Lipoproteins anchor proteins to the membrane.

Membrane Function

  • Maintains cell integrity and structure.
  • Regulates what enters and exits the cell.
  • Supports selective permeability.
  • Conserves and uses energy.

Membrane Permeability

  • A selective barrier preventing uncontrolled substance diffusion.
  • Water passes easily due to its small size (aquaporins assist).
  • Larger molecules require specific transport proteins.

Transport Proteins

  • Essential for moving substances that cannot readily diffuse across the membrane.
  • Channel proteins create pathways for water and ions.
  • Carrier proteins bind to and carry specific molecules across the membrane.

Nutrient Transport

  • Essential for acquiring necessary nutrients for growth and metabolism.
  • Specific transport systems are vital for nutrient uptake.

Archaeal Membranes

  • Differ from bacterial membranes by using phytanyl groups and ether linkages instead of ester linkages.
  • Lipids may form monolayers or bilayers.
  • Highly resistant to heat, commonly found in extreme environments.

Gram-positive vs Gram-negative Bacteria

  • Gram-positive: Thick peptidoglycan layer, contains teichoic acids.
  • Gram-negative: Thin peptidoglycan layer, outer membrane with lipopolysaccharides (LPS).

Lipopolysaccharides (LPS)

  • Component of the outer membrane of Gram-negative Bacteria.
  • Consists of Lipid A, a core polysaccharide, and O-specific polysaccharide.
  • Anchors LPS to the outer membrane.

Periplasm and Porins

  • Gel-like space between inner and outer membranes of Gram-negative bacteria.
  • Contains enzymes and proteins.
  • Porins are outer membrane proteins allowing passage of small molecules.

Cell Walls of Bacteria and Archaea

  • Peptidoglycan: Polymer made of alternating NAG and NAM, providing structural integrity and shape.

Archaeal Cell Walls

  • May have pseudomurein (differs from peptidoglycan).
  • Commonly have an S-layer (protective paracrystalline layer).

Glycocalyx

  • Thin layer surrounding the cell wall.
  • Involved in cell attachment, nutrient trapping, and protection.
  • Slime layers are a type of glycocalyx.

Fimbriae and Pili

  • Short, hairlike appendages.
  • Aid in attachment and biofilm formation.
  • Pili are longer and involved in conjugation.

Polyphosphate and Sulfur Processes

  • Energy and phosphate storage
  • Important aspects of metabolism

Microbial Locomotion

  • Flagella: Allow bacteria to move (using rotary motor for swimming).
  • Pili:involved in attachment
  • Gliding: gliding motility, a form of bacterial movement across surfaces.

Chemotaxis and Phototaxis

  • Movement towards attractants or away from repellents via chemotaxis.
  • Movement towards or away from light via phototaxis.

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