Podcast
Questions and Answers
What is the main purpose of the thermocycler in PCR?
What is the main purpose of the thermocycler in PCR?
- To separate molecules by electric current
- To measure sample concentration
- To visualize PCR results
- To alter the temperature for DNA synthesis (correct)
Gel electrophoresis separates molecules based on their electrical charge.
Gel electrophoresis separates molecules based on their electrical charge.
True (A)
Name one piece of equipment essential for measuring sample concentration.
Name one piece of equipment essential for measuring sample concentration.
Spectrophotometer
The area where samples and nucleic acids are prepared is called the ______.
The area where samples and nucleic acids are prepared is called the ______.
Which of the following is NOT a necessary component of gel electrophoresis?
Which of the following is NOT a necessary component of gel electrophoresis?
Match the laboratory equipment to their primary function:
Match the laboratory equipment to their primary function:
What is the primary objective of maintaining sterility in the culture room?
What is the primary objective of maintaining sterility in the culture room?
Cross contamination in the laboratory is unlikely to occur due to physical separation of working areas.
Cross contamination in the laboratory is unlikely to occur due to physical separation of working areas.
What does the term ‘-foresis’ in gel electrophoresis refer to?
What does the term ‘-foresis’ in gel electrophoresis refer to?
The CO2 incubator's main function is to provide humidity control for cell cultures.
The CO2 incubator's main function is to provide humidity control for cell cultures.
Name two types of microscopes used in the cytogenetics laboratory.
Name two types of microscopes used in the cytogenetics laboratory.
The __________ is used for sterilizing samples and materials through high temperatures and pressure.
The __________ is used for sterilizing samples and materials through high temperatures and pressure.
Who is commonly referred to as the father of aseptic techniques?
Who is commonly referred to as the father of aseptic techniques?
Which of the following is NOT a component of a laminar flow cabinet?
Which of the following is NOT a component of a laminar flow cabinet?
Match the laboratory equipment with its primary function:
Match the laboratory equipment with its primary function:
Sterilization only removes most active microorganisms from a surface.
Sterilization only removes most active microorganisms from a surface.
Gel electrophoresis is a technique used to amplify DNA samples.
Gel electrophoresis is a technique used to amplify DNA samples.
What are two types of risks addressed in laboratory safety?
What are two types of risks addressed in laboratory safety?
What is the role of the water purification system in a cytogenetics laboratory?
What is the role of the water purification system in a cytogenetics laboratory?
_______ is the process of using UV radiation or an autoclave to eliminate all life forms from materials.
_______ is the process of using UV radiation or an autoclave to eliminate all life forms from materials.
Match the laboratory safety types with their descriptions:
Match the laboratory safety types with their descriptions:
Which of the following is NOT a method for disinfection?
Which of the following is NOT a method for disinfection?
Proper ergonomic practices in the lab can help prevent physical safety risks.
Proper ergonomic practices in the lab can help prevent physical safety risks.
Using appropriate __________ can help prevent exposure to hazardous biological agents.
Using appropriate __________ can help prevent exposure to hazardous biological agents.
Flashcards
PCR (Polymerase Chain Reaction)
PCR (Polymerase Chain Reaction)
A technique that exponentially amplifies a DNA chain.
Pre-PCR Area
Pre-PCR Area
The area where samples and nucleic acids are prepared before PCR.
Gel Electrophoresis
Gel Electrophoresis
A process that separates DNA molecules based on size by applying an electric current.
Thermocycler
Thermocycler
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Cytogenetics
Cytogenetics
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Cross-Contamination
Cross-Contamination
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Negative Controls
Negative Controls
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Molecular Biology
Molecular Biology
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UV Transilluminator
UV Transilluminator
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Cell Culture Room
Cell Culture Room
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Spectrophotometer
Spectrophotometer
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Laminar Flow Cabinet
Laminar Flow Cabinet
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CO2 Incubator
CO2 Incubator
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Inverted Microscope
Inverted Microscope
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Fluorescence Microscope
Fluorescence Microscope
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Sterility (in a lab)
Sterility (in a lab)
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Aseptic Technique
Aseptic Technique
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Sterilization
Sterilization
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Disinfection
Disinfection
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Biosafety
Biosafety
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Chemical Safety
Chemical Safety
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Physical Safety (Lab)
Physical Safety (Lab)
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PPE (Personal Protective Equipment)
PPE (Personal Protective Equipment)
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Contamination (Control Tube)
Contamination (Control Tube)
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Study Notes
Cytogenetics and Molecular Biology Laboratory
- Cytogenetics: Studies chromosomes in dividing cells and their abnormalities.
- Molecular Biology: Studies the properties (structure, composition, and function) of cellular molecules and processes.
Laboratory Functions
- Culture Room: Perform cell cultures. Requires living, dividing cells for chromosome analysis.
- Cytogenetics Area: Perform analysis techniques.
Equipment
- Laminar Flow Hood: Filters out particles larger than 0.2 micrometers to maintain sterility. Features a transparent front panel and a mechanical system. Includes HEPA filters.
- CO2 Incubator: Maintains optimal conditions (temperature, CO2 level, humidity) for cell growth. Contains temperature, CO2, humidity controls, and air recirculation.
- Inverted Microscope: Used to observe cell culture development.
- Conventional Light Microscope: Used for standard cell staining techniques.
- Fluorescence Microscope: Used to observe fluorescently-labeled structures.
- Image Analysis System: Analyzes and records microscopy images for further study.
- Cryogenic Equipment: Stores samples in liquid nitrogen for long-term preservation.
- Autoclaves: Used to sterilize materials and samples.
- Water Purification System: Produces distilled water.
PCR Laboratory
- Pre-PCR Zone: Sample preparation (DNA).
- PCR Zone: PCR amplification process for DNA duplication.
- Post-PCR Zone: Result analysis. Includes use of a thermocycler.
- Equipment: Thermocyclers, incubators, plates, refrigerators, centrifuges allow for accurate sample management.
Material Handling and Sterilization
- Cross-Contamination: Avoidance of contamination from substances (airborne particles, personal contamination) in laboratory experiments.
- Sterilization: Processes to eliminate (or largely reduce) microorganisms.
- Chemical Methods: Employ chemicals (ethanol, sodium hypochlorite) to disinfect surfaces or materials.
- Physical Methods: Using physical methods like autoclaving and UV radiation for sterilization.
Laboratory Safety
- Biological Hazards: Protection against biological agents and toxins. Includes wearing appropriate personal protective equipment (PPE) for both workers and the environment.
- Chemical Hazards: Protection against harmful chemicals. Requires the use of appropriate equipment and personal protective measures. This focuses strictly on safety considerations for working with such products.
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Description
Explore the fundamental concepts of cytogenetics and molecular biology as applied in a laboratory setting. Learn about cell culture techniques, the analysis of chromosomes, and essential laboratory equipment. This quiz will test your knowledge on laboratory functions and the tools used for cytogenetic analysis.
