18 Questions
What is the recommended number of cells to count during cerebrospinal fluid analysis?
100 cells, classified in percentage
What is the primary benefit of adding 30% albumin to the conical chamber during cytocentrifugation?
Increases cell yield and decreases cellular distortion
What is the typical composition of cerebrospinal fluid in adults?
70% lymphocytes and 30% monocytes
What is the purpose of cytocentrifugation in cerebrospinal fluid analysis?
To concentrate the specimen
What is the main advantage of cytocentrifugation in meningitis diagnosis?
It is highly sensitive
What is the reversed proportion of lymphocytes and monocytes found in children's cerebrospinal fluid?
30% lymphocytes and 70% monocytes
Why is it important to correct the WBC count in CSF analysis?
To account for contamination from blood
What is the purpose of adding 3% glacial acetic acid to the diluting fluid?
To lyse RBCs prior to WBC count
What is the purpose of performing a differential count on a stained smear?
To identify the type of WBCs present
Why is it important to use a calibrated pipette when collecting CSF?
To ensure accurate measurements
What is the purpose of rinsing the pipette with the diluting fluid prior to sucking?
To ensure equal distribution of cells in the chamber
When is it necessary to correct the RBC count?
When a traumatic tap has occurred
What is the primary cause of parasitic invasion of the CNS worldwide?
Fungal infections (primarily Coccidiodes immitis)
What is the normal percentage of neutrophils in CSF with a normal WBC count?
Up to 7%
What type of cells are seen in CSF following diagnostic procedures such as pneumoencephalography?
Clusters of uniform cells
Which of the following is characterized by an autoimmune disorder where the myelin sheath is attacked?
Multiple sclerosis
What is the significance of finding hemosiderin granules in CSF?
May be seen 2-4 hours after RBCs enter the CSF
What is the primary function of laboratory personnel in regards to CSF analysis?
To differentiate between abnormal and normal cells
This quiz covers the methods of specimen concentration, including sedimentation, filtration, centrifugation, and cytocentrifugation. Learn about the techniques used to increase cell yield and decrease cellular distortion. Test your knowledge of laboratory procedures and cellular analysis.
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