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What is the primary purpose of culturing microorganisms in a lab?
What is the primary purpose of culturing microorganisms in a lab?
In nutrient broth solution, the flask is stoppered with rubber to prevent air from contaminating the culture.
In nutrient broth solution, the flask is stoppered with rubber to prevent air from contaminating the culture.
False (B)
What is the function of the agar in an agar gel plate?
What is the function of the agar in an agar gel plate?
culture medium
Wire loops called ______ are used to spread the microorganisms over the agar.
Wire loops called ______ are used to spread the microorganisms over the agar.
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Match the following terms with their description:
Match the following terms with their description:
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Flashcards
Culturing Microorganisms
Culturing Microorganisms
The process of growing microorganisms in a laboratory setting using nutrient-rich mediums.
Nutrient Broth
Nutrient Broth
A liquid solution containing essential nutrients that supports microbial growth. Examples include carbohydrates, minerals, proteins, and vitamins.
Agar Gel Plate
Agar Gel Plate
A solid media made from agar, a gelatinous substance derived from seaweed. Used to culture and separate bacteria in petri dishes, forming visible colonies.
Inoculating Loop
Inoculating Loop
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Sterile Technique
Sterile Technique
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Study Notes
Culturing Microorganisms
- Microorganisms are small, requiring cultivation for study.
- Culture media provides nutrients (carbohydrates, minerals, proteins, vitamins) for growth.
- Two methods exist for culturing microorganisms:
Nutrient Broth Solution
- Bacteria suspension preparation in sterile nutrient broth (culture medium).
- Flask is stoppered with cotton wool to prevent contamination and is shaken regularly for oxygen supply.
Agar Gel Plate
- Agar acts as culture medium.
- Sterilised agar jelly poured into Petri dish and allowed to cool and set.
- Inoculating loops dipped in microorganism solution and spread evenly on agar.
- Plate incubated at optimal temperature.
- Plate storage: upside down.
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Description
This quiz explores the fundamental concepts of culturing microorganisms, including the use of nutrient broth solutions and agar gel plates. You'll learn about the methods for cultivating these tiny organisms and the necessary precautions to avoid contamination. Test your knowledge of the materials and processes involved in microbiological studies.
