Culturing Microorganisms Overview

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Questions and Answers

What is the primary purpose of culturing microorganisms in a lab?

To grow a large number of them for study. (correct)
To prevent contamination.
To destroy all microorganisms.
To test their susceptibility to antibiotics.

In nutrient broth solution, the flask is stoppered with rubber to prevent air from contaminating the culture.

False (B)

What is the function of the agar in an agar gel plate?

culture medium

Wire loops called ______ are used to spread the microorganisms over the agar.

<p>inoculating loops</p> Signup and view all the answers

Match the following terms with their description:

<p>Nutrient broth = A liquid medium used to grow microorganisms. Agar gel plate = A solid medium used to grow microorganisms. Inoculating loop = A wire tool for spreading microorganisms. Culture medium = Substance containing all the necessary nutrients for microorganism growth.</p> Signup and view all the answers

Flashcards

Culturing Microorganisms

The process of growing microorganisms in a laboratory setting using nutrient-rich mediums.

Nutrient Broth

A liquid solution containing essential nutrients that supports microbial growth. Examples include carbohydrates, minerals, proteins, and vitamins.

Agar Gel Plate

A solid media made from agar, a gelatinous substance derived from seaweed. Used to culture and separate bacteria in petri dishes, forming visible colonies.

Inoculating Loop

A sterilized loop used to transfer a small sample of microorganisms onto a culture medium.

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Sterile Technique

The practice of keeping a culture medium free from contamination by unwanted microorganisms, ensuring the growth of only the intended organisms.

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Study Notes

Culturing Microorganisms

Microorganisms are small, requiring cultivation for study.
Culture media provides nutrients (carbohydrates, minerals, proteins, vitamins) for growth.
Two methods exist for culturing microorganisms:

Nutrient Broth Solution

Bacteria suspension preparation in sterile nutrient broth (culture medium).
Flask is stoppered with cotton wool to prevent contamination and is shaken regularly for oxygen supply.