Culture Media in Medical Microbiology

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Questions and Answers

Which type of media involves adding special substrates and indicators to visually differentiate between different bacteria?

  • Differential media (correct)
  • Specialized media
  • Selective media
  • Assay media

Which type of media is specifically used for sensitivity tests like antibiotic, amino acid, and vitamins?

  • Specialized media
  • Selective media
  • Differential media
  • Assay media (correct)

On MacConkey agar, how do E. coli colonies typically appear due to lactose fermentation?

  • Yellow
  • White
  • Green
  • Pink (correct)

Which type of media allows the growth of certain organisms while inhibiting the growth of others?

<p>Selective media (A)</p> Signup and view all the answers

What is the purpose of using TCBS Agar in microbiology?

<p>Isolation of specific bacteria genus like Vibrio cholera (B)</p> Signup and view all the answers

What is the main purpose of using culture media in microbiology?

<p>To provide a suitable environment for the growth of microorganisms outside the body (A)</p> Signup and view all the answers

Which component is responsible for solidifying microbiological media?

<p>Agar (B)</p> Signup and view all the answers

What nutrients are essential for the growth of microorganisms in culture media?

<p>Energy source, carbon source, nitrogen source (B)</p> Signup and view all the answers

Why is a suitable pH (around 7.2-7.4) important in culture media?

<p>To mimic the physiological conditions for microbial growth (A)</p> Signup and view all the answers

What is the role of peptone in culture media?

<p>Supply carbon and nitrogen to the media (A)</p> Signup and view all the answers

Why is it important to classify culture media based on consistency?

<p>To determine the appropriate growth conditions for specific microorganisms (D)</p> Signup and view all the answers

What is the main purpose of solid media in bacterial culture?

<p>To isolate, characterize, and identify bacteria (B)</p> Signup and view all the answers

Which agar concentration is typically found in semi-solid media?

<p>0.3-0.5% (B)</p> Signup and view all the answers

What defines an enriched medium in bacterial culture?

<p>Extra nutrients like blood or serum are added to support fastidious bacteria (B)</p> Signup and view all the answers

In the classification based on chemical components, what characterizes semi-synthetic media?

<p>Includes both natural and synthetic substances (C)</p> Signup and view all the answers

What distinguishes liquid or broth media from solid media in bacterial culture?

<p>They do not contain agar and are for the proliferation of bacteria (C)</p> Signup and view all the answers

Which type of culture media is considered a general purpose media in a lab setting?

<p>Peptone water (C)</p> Signup and view all the answers

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