Culture Media in Medical Microbiology
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Questions and Answers

Which type of media involves adding special substrates and indicators to visually differentiate between different bacteria?

  • Differential media (correct)
  • Specialized media
  • Selective media
  • Assay media
  • Which type of media is specifically used for sensitivity tests like antibiotic, amino acid, and vitamins?

  • Specialized media
  • Selective media
  • Differential media
  • Assay media (correct)
  • On MacConkey agar, how do E. coli colonies typically appear due to lactose fermentation?

  • Yellow
  • White
  • Green
  • Pink (correct)
  • Which type of media allows the growth of certain organisms while inhibiting the growth of others?

    <p>Selective media</p> Signup and view all the answers

    What is the purpose of using TCBS Agar in microbiology?

    <p>Isolation of specific bacteria genus like Vibrio cholera</p> Signup and view all the answers

    What is the main purpose of using culture media in microbiology?

    <p>To provide a suitable environment for the growth of microorganisms outside the body</p> Signup and view all the answers

    Which component is responsible for solidifying microbiological media?

    <p>Agar</p> Signup and view all the answers

    What nutrients are essential for the growth of microorganisms in culture media?

    <p>Energy source, carbon source, nitrogen source</p> Signup and view all the answers

    Why is a suitable pH (around 7.2-7.4) important in culture media?

    <p>To mimic the physiological conditions for microbial growth</p> Signup and view all the answers

    What is the role of peptone in culture media?

    <p>Supply carbon and nitrogen to the media</p> Signup and view all the answers

    Why is it important to classify culture media based on consistency?

    <p>To determine the appropriate growth conditions for specific microorganisms</p> Signup and view all the answers

    What is the main purpose of solid media in bacterial culture?

    <p>To isolate, characterize, and identify bacteria</p> Signup and view all the answers

    Which agar concentration is typically found in semi-solid media?

    <p>0.3-0.5%</p> Signup and view all the answers

    What defines an enriched medium in bacterial culture?

    <p>Extra nutrients like blood or serum are added to support fastidious bacteria</p> Signup and view all the answers

    In the classification based on chemical components, what characterizes semi-synthetic media?

    <p>Includes both natural and synthetic substances</p> Signup and view all the answers

    What distinguishes liquid or broth media from solid media in bacterial culture?

    <p>They do not contain agar and are for the proliferation of bacteria</p> Signup and view all the answers

    Which type of culture media is considered a general purpose media in a lab setting?

    <p>Peptone water</p> Signup and view all the answers

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