Podcast
Questions and Answers
What binds to the operator to prevent transcription?
What binds to the operator to prevent transcription?
What happens to the lac repressor protein when glucose is present?
What happens to the lac repressor protein when glucose is present?
Where does the RNA polymerase bind to initiate transcription?
Where does the RNA polymerase bind to initiate transcription?
What is the role of cAMP in regulating the lac operon?
What is the role of cAMP in regulating the lac operon?
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What happens when the CRP-cAMP complex binds to the CRP-binding site?
What happens when the CRP-cAMP complex binds to the CRP-binding site?
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Where is the genetic code for the lac repressor protein found?
Where is the genetic code for the lac repressor protein found?
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What activates the CRP protein in E. coli?
What activates the CRP protein in E. coli?
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What happens as glucose levels drop in E. coli?
What happens as glucose levels drop in E. coli?
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What role does CRP play when bound to cAMP?
What role does CRP play when bound to cAMP?
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What is the result when lactose levels are high in E. coli?
What is the result when lactose levels are high in E. coli?
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What is true when glucose levels are high?
What is true when glucose levels are high?
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Which condition leads to activation of the lac operon?
Which condition leads to activation of the lac operon?
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Study Notes
Allosteric Activation of CRP
- Inactive CRP protein is converted to active CRP protein by binding to cAMP
- cAMP binds to CRP, enabling it to bind to a special DNA sequence adjacent to the RNA polymerase binding site
- This activation allows RNA polymerase to bind to the lac operon
Cyclic AMP and Glucose
- In E. coli, ATP is converted to cAMP by adenylate cyclase
- As glucose levels drop, cAMP levels increase
- cAMP binds to CRP, leading to transcription activation
Positive Regulation of Transcription
- A bound activator (CRP-cAMP complex) facilitates transcription by binding to specific DNA sequences
- This type of regulation is seen in the lac operon, where CRP-cAMP complex binds to the lac promoter, enhancing transcription
Regulation of the lac Operon
- In the absence of lactose, the repressor is bound, inhibiting expression
- When lactose is present, the repressor dissociates, allowing transcription
- High glucose levels prevent CRP binding, dampening transcription
- Low glucose levels increase cAMP, leading to CRP binding and activation of transcription
lac Operon Structure
- The lac operon has an operator site where the lac repressor protein binds, preventing transcription
- The lac promoter has a CRP-binding site where the CRP-cAMP complex binds, enhancing transcription
- The lac mRNA contains the genetic code for the lac repressor protein
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Description
This quiz covers the role of cAMP and CRP in gene regulation in E. coli, including allosteric activation and transcription activation.
