5 Questions
Which gene does the Arabidopsis CBF4 gene encode?
AP2/ERF family transcription factor
What is the purpose of generating a cbf4 knock-out mutant?
To analyze mutant phenotype
Which technology is used to clone sgRNA in CRISPR expression vector?
Golden Gate cloning
What is the next step after transforming E. coli with the CRISPR expression vector?
Transform Agrobacterium tumefaciens
What is the purpose of generating a CBF4-YFP fusion protein?
To determine protein localization and overexpression phenotype
Test your knowledge of genome editing using CRISPR-Cas technology and explore the crystal structure of the Staphylococcus aureus Cas9 complex. Also, learn about the function of the Arabidopsis CBF4 gene and how to study it in BIOD21.
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