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Questions and Answers
Which gene does the Arabidopsis CBF4 gene encode?
Which gene does the Arabidopsis CBF4 gene encode?
- CRISPR-Cas9 complex
- AP2/ERF family transcription factor (correct)
- Staphylococcus aureus Cas9 complex
- Golden Gate cloning
What is the purpose of generating a cbf4 knock-out mutant?
What is the purpose of generating a cbf4 knock-out mutant?
- To determine protein localization
- To study the function of the CRISPR-Cas9 complex
- To analyze mutant phenotype (correct)
- To clone sgRNA in CRISPR expression vector
Which technology is used to clone sgRNA in CRISPR expression vector?
Which technology is used to clone sgRNA in CRISPR expression vector?
- Gateway recombination technology
- CRISPR-Cas9 complex
- Staphylococcus aureus Cas9 complex
- Golden Gate cloning (correct)
What is the next step after transforming E. coli with the CRISPR expression vector?
What is the next step after transforming E. coli with the CRISPR expression vector?
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What is the purpose of generating a CBF4-YFP fusion protein?
What is the purpose of generating a CBF4-YFP fusion protein?
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