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CRISPR-Cas Technology and Gene Editing

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Questions and Answers

Which gene does the Arabidopsis CBF4 gene encode?

CRISPR-Cas9 complex

AP2/ERF family transcription factor (correct)

Staphylococcus aureus Cas9 complex

Golden Gate cloning

What is the purpose of generating a cbf4 knock-out mutant?

To determine protein localization

To study the function of the CRISPR-Cas9 complex

To analyze mutant phenotype (correct)

To clone sgRNA in CRISPR expression vector

Which technology is used to clone sgRNA in CRISPR expression vector?

Gateway recombination technology

CRISPR-Cas9 complex

Staphylococcus aureus Cas9 complex

Golden Gate cloning (correct)

What is the next step after transforming E. coli with the CRISPR expression vector?

<p>Transform Agrobacterium tumefaciens</p> Signup and view all the answers

What is the purpose of generating a CBF4-YFP fusion protein?

<p>To determine protein localization and overexpression phenotype</p> Signup and view all the answers

CRISPR-Cas Technology and Gene Editing

Choose a study mode

Podcast

Questions and Answers

Which gene does the Arabidopsis CBF4 gene encode?

What is the purpose of generating a cbf4 knock-out mutant?

Which technology is used to clone sgRNA in CRISPR expression vector?

What is the next step after transforming E. coli with the CRISPR expression vector?

What is the purpose of generating a CBF4-YFP fusion protein?

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