COVID-19 and Alzheimer's Disease

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What was observed in Western blots from brain lysates of COVID-19 patients?

Increased RyR2 oxidation and PKA phosphorylation (correct)
No changes in RyR2 oxidation and PKA phosphorylation
Decreased PKA phosphorylation
Reduced calstabin2 and NOX2 bound to the channel

What was the method used to determine oxidative stress levels in brain tissues?

Western blots depicting RyR2 oxidation
Measuring the ratio of GSSG to GSH by an ELISA kit (correct)
3 [H]ryanodine binding from immunoprecipitated RyR2
TMB substrate incubation

What was the purpose of adding TMB substrate to each well?

To measure the ratio of GSSG to GSH
To incubate the plate for 30 minutes at RT
To aspirate the 3.2.1 Oxidative stress plate
To determine the absorbance at 450 nm (correct)

Which of the following proteins was found to be dysregulated in COVID-19 brains?

Ryanodine receptor 2 (RyR2) (C) Signup and view all the answers

What was the comparison made to determine the sample signals?

Comparison to a standard curve (D) Signup and view all the answers

What was the significance of the data obtained from the Western blots?

Data showed a significant difference between control and COVID-19 samples (B) Signup and view all the answers

What is the main mechanism of cognitive impairment associated with COVID-19?

Oxidative stress (C) Signup and view all the answers

Which of the following is NOT a therapeutic target for COVID-19 neurological complications?

Ryanodine receptor/calcium release channels (RyR) (A) Signup and view all the answers

What is the role of TGF-β signaling in COVID-19?

Activating Alzheimer's disease-like signaling (A) Signup and view all the answers

What is the name of the protein associated with Alzheimer's disease-like signaling in COVID-19 patients?

Ryanodine receptor (RyR) (A) Signup and view all the answers

What is the effect of SARS-CoV-2 infection on the brain?

Activating TGF-β signaling (C) Signup and view all the answers

What is the consequence of oxidative overload in COVID-19 patients?

Increased risk of Alzheimer's disease (A) Signup and view all the answers

What is proposed as a potential therapeutic target for ameliorating COVID-19 neurological symptoms?

Defective intracellular Ca2+ regulation (C) Signup and view all the answers

What is associated with fibrosis in the lungs and other organs?

TGF-β activation (B) Signup and view all the answers

What is a potential risk factor for developing AD in the future?

Prior COVID-19 infection (D) Signup and view all the answers

What is reported to be upregulated in COVID-19 infection?

Genes involved in oxidative overload (D) Signup and view all the answers

What is suggested to explain the coordination deficits experienced by hospitalized COVID-19 patients?

Tau protein pathology in the cerebellum (A) Signup and view all the answers

What is involved in the formation of cellular fibrosis?

TGF-β (B) Signup and view all the answers