Connective Tissue: medium level

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Questions and Answers

Connective tissue proper, cartilage, and bone share the same building plan.

True (A)

The extracellular matrix of connective tissue proper contains a homogenous solid substance.

False (B)

Transient cells in connective tissue proper originate mainly from chondroblastic stem cells.

False (B)

Fixed cells in connective tissue proper originate from undifferentiated neuroglial cells.

<p>False (B)</p> Signup and view all the answers

Elastic membranes in the aorta can be stained using thionin.

<p>False (B)</p> Signup and view all the answers

Collagen fibers in loose collagenous connective tissue can be demonstrated using anilin blue staining.

<p>True (A)</p> Signup and view all the answers

The three main types of connective tissue are connective tissue proper, muscle, and bone.

<p>False (B)</p> Signup and view all the answers

Collagen is a component of the fibrillar part of the extracellular matrix.

<p>True (A)</p> Signup and view all the answers

The extracellular matrix contains only fibers and no ground substance.

<p>False (B)</p> Signup and view all the answers

Fixed cells in connective tissue leave the bloodstream to migrate into the connective tissue.

<p>False (B)</p> Signup and view all the answers

Mucous connective tissue is typically found in the brain of an adult.

<p>False (B)</p> Signup and view all the answers

Leukocytes originate from hemopoietic stem cells.

<p>True (A)</p> Signup and view all the answers

Connective tissue proper consists only of the extracellular matrix.

<p>False (B)</p> Signup and view all the answers

Plasma cells differentiate from adipocytes.

<p>False (B)</p> Signup and view all the answers

Adipose tissue is a type of muscle tissue.

<p>False (B)</p> Signup and view all the answers

Reticulin can be used to impregnate the tissue in reticular connective tissue.

<p>True (A)</p> Signup and view all the answers

Mucous connective tissue in the umbilical cord is stained using hematoxylin and eosin.

<p>False (B)</p> Signup and view all the answers

Mast cells are stained using thionin.

<p>True (A)</p> Signup and view all the answers

Dense collagenous connective tissue regular is found in the skin.

<p>False (B)</p> Signup and view all the answers

Elastic membranes are demonstrated in the aorta with aniline blue.

<p>False (B)</p> Signup and view all the answers

Reticular fibers are commonly found in dense collagenous connective tissue regular.

<p>False (B)</p> Signup and view all the answers

Ground substance, an amorphous component of the extracellular matrix, does not contain glycoproteins.

<p>False (B)</p> Signup and view all the answers

Elastic fibers provide rigid support to connective tissue proper.

<p>False (B)</p> Signup and view all the answers

Fibroblasts are transient cells within connective tissue proper.

<p>False (B)</p> Signup and view all the answers

Adipocytes found in adipose tissue are responsible for producing elastic fibers.

<p>False (B)</p> Signup and view all the answers

Collagen fibers are arranged irregularly in dense collagenous connective tissue found in tendons.

<p>False (B)</p> Signup and view all the answers

Loose areolar collagenous connective tissue is found in the mucosal and submucosal connective tissue of the large intestine.

<p>False (B)</p> Signup and view all the answers

Plasma cells in loose connective tissue produce elastic fibers.

<p>False (B)</p> Signup and view all the answers

The primary function of reticular connective tissue is to provide cushioning and insulation.

<p>False (B)</p> Signup and view all the answers

Mucous connective tissue, present in the umbilical cord, is characterized by abundant collagen fibers.

<p>True (A)</p> Signup and view all the answers

The three main types of connective tissue proper are: collagenous, reticular and mucous.

<p>False (B)</p> Signup and view all the answers

Elastic membranes in the aorta are composed primarily of fibronectin.

<p>False (B)</p> Signup and view all the answers

The walls of arteries may include elastic membranes, which aid in withstanding pressure changes.

<p>True (A)</p> Signup and view all the answers

Adipocytes, responsible for fat storage in adipose tissue, are derived from hematopoietic stem cells.

<p>False (B)</p> Signup and view all the answers

The amorphous ground substance's primary role is to give color to cutaneous tissue.

<p>False (B)</p> Signup and view all the answers

While orcein is used to stain elastic membranes, it's typically ineffective for staining collagen fibers.

<p>True (A)</p> Signup and view all the answers

The orientation of collagen fibers in dense regular connective tissue facilitates multidirectional stretching.

<p>False (B)</p> Signup and view all the answers

Plasma cells, derived from T lymphocytes, differentiate to produce antibodies.

<p>False (B)</p> Signup and view all the answers

Reticular connective tissue, offering a unique framework, is predominant in the liver.

<p>False (B)</p> Signup and view all the answers

Thionin is used to stain plasma cells

<p>False (B)</p> Signup and view all the answers

Connective tissue proper (CTP) is structured with both transient cells, originating from mesenchymal stem cells, and fixed cells, originating from hematopoietic stem cells.

<p>False (B)</p> Signup and view all the answers

Collagenous CTP can be classified as either loose or serous, depending on the organization of its collagen fibers.

<p>False (B)</p> Signup and view all the answers

In dense regular collagenous connective tissue, such as a tendon, the nuclei of fibroblasts appear oval-shaped when viewed under Hematoxylin and Eosin (HE) staining.

<p>False (B)</p> Signup and view all the answers

Plasma cells, a type of transient cell in connective tissue proper, originate from T lymphocytes and are commonly found in loose collagenous connective tissue.

<p>False (B)</p> Signup and view all the answers

Elastic membranes in the aorta are best visualized using periodic acid-Schiff (PAS) staining.

<p>False (B)</p> Signup and view all the answers

Flashcards

Connective Tissue

A type of connective tissue that includes connective tissue proper (CTP), cartilage, and bone.

Connective Tissue Proper (CTP)

A sub-type of connective tissue, distinct from cartilage and bone

Connective Tissue Structure

The structure consists of cells (fixed and transient) and the extracellular matrix (amorphous ground substance and fibers).

Fixed Cells of CTP

Originate from undifferentiated embryonic mesenchymal cells; includes fibroblasts/fibrocytes, reticular cells, pigment cells, and fat cells (adipocytes).

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Transient (free) cells of CTP

Originate mostly from hematopoietic stem cells of the bone marrow; include mast cells, plasma cells, macrophages, and leukocytes.

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Amorphous Ground Substance

Highly hydrated, colorless, and transparent substance, a complex mixture of glycosaminoglycans, proteoglycans, and glycoproteins.

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Fibers of the Extracellular Matrix

Includes collagen, elastic, and reticular fibers.

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Types of Connective Tissue Proper

Collagenous, elastic, reticular, mucous and adipose are the types of?

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Collagenous CTP

Type of CTP with regular and irregular subtypes.

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Reticular CTP

Type of CTP that is found in lymph nodes.

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Mucous CTP

This is connective tissue in umbilical cord

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Adipose CTP

Consists of white and brown subtypes.

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Thionin Stain

Common stain used to demonstrate mast cells in loose collagenous connective tissue.

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Hematoxylin and eosin

Common stains used to demonstrate plasma cells

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Anilin Blue

Common stain used to demonstrate collagen fibers in loose collagenous connective tissue.

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Orcein Stain

Stain used to demonstrate elastic membranes in aorta.

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Loose areolar connective tissue

Hematoxylin and eosin (HE) is used to identify the

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Dense Collagenous Connective Tissue

Hematoxylin and Eosin (HE) is used to identify regular tendon

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Dense Collagenous Connective Tissue Irregular

Green Trichrome is used to demonstrate

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Reticular Connective Tissue

Silver salts are used identify?

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Adipose Tissue

Hematoxylin and eosin (HE) is used to identify

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Mucous Connective Tissue

Green Trichrome is used to demonstrate

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Study Notes

Connective Tissue Types

  • Three types include connective tissue proper, cartilage and bone.
  • These are built according to the same base plan.
  • Connective tissue structure consists of cells and an extracellular matrix.

Connective Tissue Proper - CTP Structure

  • CTP contains fixed and transient cells, and an extracellular matrix.
  • Fixed cells originate from undifferentiated mesenchymal cells.
  • Transient cells originate from hematopoietic stem cells.

Cells in CTP

  • Fixed CTP cells originate from undifferentiated embryonic mesenchymal cells and stay within the connective tissue proper.
  • Fibroblasts/fibrocytes, reticular cells, pigment cells, and fat cells(adipocytes) are fixed cells.
  • Transient (free) CTP cells originate mostly from hemopoietic stem cells of bone marrow.
  • Free CTP cells leave the bloodstream, migrating into the loose connective tissue.
  • Mast cells, plasma cells, macrophages, and leukocytes (eosinophils and neutrophils) are transient cells.
  • Plasma cells are derived from B lymphocytes.

Extracellular Matrix

  • The amorphous ground substance is highly hydrated, colorless,and transparent.
  • It's a complex mixture of glycosaminoglycans, proteoglycans, and glycoproteins.
  • The fibrillar part has three types of fibers: collagen, elastic, and reticular.

Types of CTP

  • Types include loose collagenous, dense collagenous, elastic, and reticular.
  • Dense collagenous CTP can be regular or irregular.
  • Additional types are mucous and adipose CTP.
  • Adipose CTP types consist of white and brown adipose tissues.

Slides

  • Demonstrations include mast cells (thionin) and plasma cells (HE) in loose collagenous connective tissue proper.
  • Collagen fibers are visible in loose collagenous connective tissue proper (anilin blue).
  • Elastic membranes can be seen in the aorta (orcein).
  • Loose collagenous connective tissue proper is observed in the mucosal and submucosal connective tissue of a rat's small intestine(HE).
  • Dense collagenous connective tissue is visible as regular in tendon (HE) and regular in skin (trichrome).
  • Reticular connective tissue appears in the lymph node (impregnation of silver salts-reticuline).
  • Adipose tissue (HE) and Mucous connective tissue in the umbilical cord (trichrome) can also be observed.

Mast Cells

  • Important region localisation demonstrated in loose collagenous connective tissue with thionin.

Plasma Cells

  • Plasma cells are demonstrated in loose collagenous connective tissue, using Hematoxylin and Eosin (HE) staining.

Collagen Fibers

  • Collagen fibers in loose collagenous connective tissue are highlightable using Anilin blue staining.

Elastic Membranes

  • Elastic membranes in cross-sections of the aorta wall can be demonstrated with orcein.

Loose Areolar Collagenous Connective Tissue

  • It's found in the mucosal and submucosal connective tissue of a small intestine in rats, highlighted via Hematoxylin and Eosin (HE).

Dense Collagenous Connective Tissue

  • Regular arrangement is seen in tendons using Hematoxylin and Eosin (HE).
  • Irregular arrangement is seen in skin using trichrome staining.

Reticular Connective Tissue

  • Reticular fibers in lymph nodes are demonstrated via impregnation with silver salts (AgNO3).

Adipose Tissue

  • Can be stained by(HE).
  • Adipocytes have a univacuolar type with a nucleus.

Mucous Connective Tissue

  • Found in the umbilical cord, stained with green trichrome.
  • Collagen fibers appear green in this tissue.

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