Confirmation of Amyloid Fibrils Quiz

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How can the presence of amyloid fibrils be confirmed?

Congo red binding leading to red birefringence under polarized light

Recognition of apple-green birefringence under polarized light after Congo red staining (correct)

Appearance on light microscopy

Thioflavine-T binding producing a blue fluorescence

Which staining technique can determine the type of amyloidosis?

Hematoxylin and eosin staining

Gram staining

Immunohistochemical staining of kappa and lambda light chains (correct)

Periodic acid-Schiff staining

How can cardiac involvement in amyloidosis be suspected?

Low platelet count

Presence of fever

Arrhythmia or heart block on electrocardiogram (correct)

Elevated white blood cell count

What is a common feature of patients with primary nerve, kidney, or liver involvement in amyloidosis?

Restrictive cardiomyopathy Signup and view all the answers

Which imaging technique can help detect restrictive cardiomyopathy related to amyloidosis?

MRI Signup and view all the answers

Which clinical suspicion may remain if evaluation for other causes of symptoms is unrevealing?

Monoclonal plasma cell proliferative disorder Signup and view all the answers

What characteristic appearance of amyloid fibrils can be observed under polarized light after binding to Congo red?

Green birefringence Signup and view all the answers

Why might biopsy sections less than 6 microns thick not stain appropriately with Congo red despite the presence of amyloid fibrils?

Congo red has a limited capacity to bind to thin sections Signup and view all the answers

Which method is preferred for determining the type of amyloidosis once the histologic diagnosis of amyloid is made?

Mass spectrometry Signup and view all the answers

What is the characteristic appearance of amyloid fibrils under electron microscopy?

Fibrillar and non-branching structure Signup and view all the answers

Which staining method produces an intense yellow-green fluorescence in the presence of amyloid?

Thioflavine-T Signup and view all the answers

What is the characteristic appearance of amyloid under hematoxylin and eosin staining on biopsy sections?

Pink, amorphous, waxy substance Signup and view all the answers

What is the typical diameter of amyloid fibrils seen in renal amyloidosis?

7 to 12 nanometers Signup and view all the answers

Where are the mesangial cells and mesangial matrix usually located in a capillary tuft affected by amyloidosis?

In the central or stalk regions of the tuft Signup and view all the answers

What is the similarity between the thickness of the glomerular capillary wall and the tubular basement membranes in amyloidosis?

They are of similar thickness Signup and view all the answers

Which staining method is typically used to visualize amyloid deposits under a microscope?

Congo red stain Signup and view all the answers

In which type of glomerulonephritis are the amyloid fibrils smaller compared to those seen in renal amyloidosis?

Fibrillary glomerulonephritis Signup and view all the answers

What is the typical number of cells per capillary tuft in a case of renal amyloidosis?

One to three cells Signup and view all the answers