18 Questions
What is the main limitation of constitutive knock-out models?
Embryonic lethality associated to the deletion of some genes
What is the purpose of conditional knock-out technology?
To bypass embryonic lethality associated to gene knock-out
What is the site-specific recombinase technology used in conditional knock-out?
Cre-LoxP
What is the function of LoxP sites in the Cre-LoxP system?
To recognize Cre recombinase
What is the minimum number of mouse strains required for conditional knock-out?
Two
In what year was the conditional knock-out technology published in Science?
1995
What is the purpose of injecting ES cells into a blastocyst?
To generate a pure colony of mice with a specific genetic trait
What is the result of breeding chimeric mice?
A mix of wild-type, heterozygous, and homozygous mice
What is a common issue with gene knockouts?
All of the above
What is the result of disrupting the MYB allele (exon 5-8 removed)?
A mouse with embryonic lethality at E15.5
Why are concerns raised about the use of ES cells in gene knockout experiments?
They raise concerns with animalist and religious organizations
What is the purpose of breeding mice to obtain different genetic combinations?
To establish a colony of wild-type, heterozygous, and homozygous mice
What is the primary focus of genetic engineering?
The direct manipulation of an organism's genes using biotechnology
Which of the following animal models is commonly used for genetic manipulation?
All of the above
What is the purpose of transgenic animals in research labs?
To dissect with great precision the function and contribution of several genes to fundamental biological processes
Why are cell lines often not very useful models?
They have accumulated other mutations rather than the original one
What is the main advantage of using animal models in biomedical research?
They allow to understand the pathogenesis of human diseases at cellular and molecular levels
What is the purpose of genetic engineering in organisms?
To produce improved or novel organisms
Learn about conditional knock-out technology, a method that allows for the inducible knock-out of a gene in specific cell types, lineages, tissues, or the whole body. This method bypasses embryonic lethality associated with constitutive knock-out models. Understand the principles and advantages of conditional knock-out technology.
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