Podcast
Questions and Answers
What is the main limitation of constitutive knock-out models?
What is the main limitation of constitutive knock-out models?
- Tissue-specific deletion
- Inducible deletion
- Embryonic lethality associated to the deletion of some genes (correct)
- Time-specific deletion
What is the purpose of conditional knock-out technology?
What is the purpose of conditional knock-out technology?
- To generate transgenic organisms
- To develop retrovirus vectors
- To insert genes into embryonic stem cells
- To bypass embryonic lethality associated to gene knock-out (correct)
What is the site-specific recombinase technology used in conditional knock-out?
What is the site-specific recombinase technology used in conditional knock-out?
- LoxP-Cre
- Cre-Phage
- Phage-Cre
- Cre-LoxP (correct)
What is the function of LoxP sites in the Cre-LoxP system?
What is the function of LoxP sites in the Cre-LoxP system?
What is the minimum number of mouse strains required for conditional knock-out?
What is the minimum number of mouse strains required for conditional knock-out?
In what year was the conditional knock-out technology published in Science?
In what year was the conditional knock-out technology published in Science?
What is the purpose of injecting ES cells into a blastocyst?
What is the purpose of injecting ES cells into a blastocyst?
What is the result of breeding chimeric mice?
What is the result of breeding chimeric mice?
What is a common issue with gene knockouts?
What is a common issue with gene knockouts?
What is the result of disrupting the MYB allele (exon 5-8 removed)?
What is the result of disrupting the MYB allele (exon 5-8 removed)?
Why are concerns raised about the use of ES cells in gene knockout experiments?
Why are concerns raised about the use of ES cells in gene knockout experiments?
What is the purpose of breeding mice to obtain different genetic combinations?
What is the purpose of breeding mice to obtain different genetic combinations?
What is the primary focus of genetic engineering?
What is the primary focus of genetic engineering?
Which of the following animal models is commonly used for genetic manipulation?
Which of the following animal models is commonly used for genetic manipulation?
What is the purpose of transgenic animals in research labs?
What is the purpose of transgenic animals in research labs?
Why are cell lines often not very useful models?
Why are cell lines often not very useful models?
What is the main advantage of using animal models in biomedical research?
What is the main advantage of using animal models in biomedical research?
What is the purpose of genetic engineering in organisms?
What is the purpose of genetic engineering in organisms?
