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Conditional Knock-out Technology

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Questions and Answers

What is the main limitation of constitutive knock-out models?

Tissue-specific deletion

Inducible deletion

Embryonic lethality associated to the deletion of some genes (correct)

Time-specific deletion

What is the purpose of conditional knock-out technology?

To generate transgenic organisms

To develop retrovirus vectors

To insert genes into embryonic stem cells

To bypass embryonic lethality associated to gene knock-out (correct)

What is the site-specific recombinase technology used in conditional knock-out?

LoxP-Cre

Cre-Phage

Phage-Cre

Cre-LoxP (correct)

What is the function of LoxP sites in the Cre-LoxP system?

To recognize Cre recombinase Signup and view all the answers

What is the minimum number of mouse strains required for conditional knock-out?

Two Signup and view all the answers

In what year was the conditional knock-out technology published in Science?

1995 Signup and view all the answers

What is the purpose of injecting ES cells into a blastocyst?

To generate a pure colony of mice with a specific genetic trait Signup and view all the answers

What is the result of breeding chimeric mice?

A mix of wild-type, heterozygous, and homozygous mice Signup and view all the answers

What is a common issue with gene knockouts?

All of the above Signup and view all the answers

What is the result of disrupting the MYB allele (exon 5-8 removed)?

A mouse with embryonic lethality at E15.5 Signup and view all the answers

Why are concerns raised about the use of ES cells in gene knockout experiments?

They raise concerns with animalist and religious organizations Signup and view all the answers

What is the purpose of breeding mice to obtain different genetic combinations?

To establish a colony of wild-type, heterozygous, and homozygous mice Signup and view all the answers

What is the primary focus of genetic engineering?

The direct manipulation of an organism's genes using biotechnology Signup and view all the answers

Which of the following animal models is commonly used for genetic manipulation?

All of the above Signup and view all the answers

What is the purpose of transgenic animals in research labs?

To dissect with great precision the function and contribution of several genes to fundamental biological processes Signup and view all the answers

Why are cell lines often not very useful models?

They have accumulated other mutations rather than the original one Signup and view all the answers

What is the main advantage of using animal models in biomedical research?

They allow to understand the pathogenesis of human diseases at cellular and molecular levels Signup and view all the answers

What is the purpose of genetic engineering in organisms?

To produce improved or novel organisms Signup and view all the answers