Podcast
Questions and Answers
What is the primary function of tight junctions in epithelial cells?
What is the primary function of tight junctions in epithelial cells?
- To serve as barriers to solute diffusion (correct)
- To facilitate cell migration
- To enable solute diffusion
- To regulate cell proliferation
What is the role of ZO proteins in tight junctions?
What is the role of ZO proteins in tight junctions?
- To regulate cell migration
- To bind to claudins and occludins
- To facilitate cell-cell adhesion
- To act as a scaffold (correct)
What is the primary function of cadherins in epithelial cells?
What is the primary function of cadherins in epithelial cells?
- To facilitate solute diffusion
- To regulate cell migration
- To mediate heterophilic interactions with the ECM
- To mediate homophilic cell-cell adhesion (correct)
What is the role of integrins in epithelial cells?
What is the role of integrins in epithelial cells?
What is the primary component of the ECM that provides structural support?
What is the primary component of the ECM that provides structural support?
What is the process by which lymphocytes migrate into tissues?
What is the process by which lymphocytes migrate into tissues?
What is the primary component of ECM produced by fibroblasts?
What is the primary component of ECM produced by fibroblasts?
What percentage of skin is composed of collagen?
What percentage of skin is composed of collagen?
How many types of collagen proteins exist?
How many types of collagen proteins exist?
What is the sequence found in all collagens?
What is the sequence found in all collagens?
What is the typical structure of an α chain?
What is the typical structure of an α chain?
What is the function of proline in collagen?
What is the function of proline in collagen?
How are collagen polypeptide α chains arranged in a triple helix?
How are collagen polypeptide α chains arranged in a triple helix?
Where is post-translational modification of collagen chains carried out?
Where is post-translational modification of collagen chains carried out?
What is unique about the structure of matrix proteoglycans and glycosaminoglycans?
What is unique about the structure of matrix proteoglycans and glycosaminoglycans?
Which cells are responsible for secreting matrix macromolecules in connective tissues?
Which cells are responsible for secreting matrix macromolecules in connective tissues?
What is the primary function of the ECM in connective tissues?
What is the primary function of the ECM in connective tissues?
What is the composition of the basal lamina different from?
What is the composition of the basal lamina different from?
What is the primary function of the basal lamina?
What is the primary function of the basal lamina?
What is the focus of the video series covered in Module 8?
What is the focus of the video series covered in Module 8?
What is the primary function of the TGN in polarised epithelial cells?
What is the primary function of the TGN in polarised epithelial cells?
What is the mechanism of protein transport in the indirect pathway?
What is the mechanism of protein transport in the indirect pathway?
What is the role of lipid rafts in protein transport?
What is the role of lipid rafts in protein transport?
What is the function of the cytoplasmic tail of a protein?
What is the function of the cytoplasmic tail of a protein?
What is the direction of protein transport in the direct pathway?
What is the direction of protein transport in the direct pathway?
What is the role of the antibody to the cytoplasmic tail of the blue cargo protein?
What is the role of the antibody to the cytoplasmic tail of the blue cargo protein?
Where do all newly synthesized proteins go first in polarised epithelial cells?
Where do all newly synthesized proteins go first in polarised epithelial cells?
Where does endocytosis occur in polarised epithelial cells?
Where does endocytosis occur in polarised epithelial cells?
Which type of protein facilitates direct contacts between the ECM and cells via cell surface receptors?
Which type of protein facilitates direct contacts between the ECM and cells via cell surface receptors?
What is the primary function of integrins in relation to the ECM?
What is the primary function of integrins in relation to the ECM?
What is the role of multiple binding domains in adhesive glycoproteins?
What is the role of multiple binding domains in adhesive glycoproteins?
Which of the following is a characteristic of adhesive glycoproteins in the ECM?
Which of the following is a characteristic of adhesive glycoproteins in the ECM?
What is the role of actin cytoskeleton in the formation of integrin-ECM interactions?
What is the role of actin cytoskeleton in the formation of integrin-ECM interactions?
Which of the following ECM components is involved in the formation of direct contacts between the ECM and cells?
Which of the following ECM components is involved in the formation of direct contacts between the ECM and cells?
What is the role of integrins in the migration of lymphocytes into tissues?
What is the role of integrins in the migration of lymphocytes into tissues?
What is the function of fibronectin and laminin in the ECM?
What is the function of fibronectin and laminin in the ECM?
Flashcards are hidden until you start studying
Study Notes
Experimental Rationales for Sorting Mechanisms in Epithelial Cells
- Experimental approaches to distinguish different sorting mechanisms in epithelial cells involve understanding how membrane proteins reach the correct surface of polarized epithelial cells.
- Two pathways exist: direct sorting of membrane proteins in the TGN (Trans-Golgi Network) and indirect sorting via endocytosis and selective transport to the apical surface.
Integrating Cells into Organized Tissues
- The extracellular matrix (ECM) plays a crucial role in integrating cells into organized tissues.
- The ECM is made and oriented by the cells within it, bearing mechanical stresses of tension and compression.
Extracellular Matrix (ECM)
- The ECM is a highly dynamic structure continually produced and remodeled.
- The ECM composition of basal laminae is different from connective tissue.
- The ECM is made up of various components, including collagen, proteoglycans, and glycosaminoglycans.
Collagen and Collagen Fibres
- Collagen is a major fibrillar component of the ECM, found in various tissues such as cartilage, cornea, bone, and skin.
- There are 42 types of collagen, all containing the Gly-Xaa-Yaa-sequon in tandem (10-90% of sequence).
- Collagen has a triple helix structure, with three collagen polypeptide α chains wound around each other.
- Proline stabilizes the helical conformation, while glycine fits into the crowded interior, allowing tight packing of the triple helix.
Adhesive Glycoproteins of the ECM
- Adhesive ECM glycoproteins, such as fibronectin and laminin, form direct contacts between the ECM and cells via cell surface receptors (integrins).
- These glycoproteins contain multiple binding domains and interact with cell surface receptors, localizing fibronectin/laminin at appropriate sites for assembly and increasing local protein concentration at the cell surface.
Cell-Cell Adhesion and Integrins
- Cadherins mediate homophilic cell-cell adhesion, with a specific structure and molecular interactions.
- Integrins mediate heterophilic interactions between cells and the ECM, with a specific structure, molecular interactions, and regulation.
- Integrins play a crucial role in lymphocyte migration into tissues.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
