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Cold Sterilization Methods in Pharmaceutical Microbiology

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Cold Sterilization Methods in Pharmaceutical Microbiology

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Questions and Answers

What is the main mechanism of action of ethylene oxide gas in sterilization?

Denaturation of microbial enzymes

Inhibition of microbial DNA replication

Oxidation of microbial cell membranes

Alkylation of hydroxyl and carboxyl groups of proteins and nucleic acids (correct)

What is the recommended concentration range for ethylene oxide gas in sterilization?

100-300 mg/L

400-600 mg/L

2000-2500 mg/L

800-1200 mg/L (correct)

At what relative humidity level does the activity of ethylene oxide gas decrease?

> 60%

< 30% (correct)

40-50%

< 10%

Which material does ethylene oxide gas not penetrate during sterilization?

<p>Glass</p> Signup and view all the answers

What is the temperature range for ethylene oxide gas in sterilization?

<p>45-63°C</p> Signup and view all the answers

Study Notes

Ethylene Oxide Gas Sterilization

The main mechanism of action of ethylene oxide gas is alkylating cell components, particularly DNA, to disrupt microbial metabolism and eventually kill microorganisms.
The recommended concentration range for ethylene oxide gas in sterilization is 450-1200 mg/L.
The activity of ethylene oxide gas decreases at a relative humidity level below 30%.
Ethylene oxide gas does not penetrate certain materials, such as rubber and some plastics, during sterilization.
The temperature range for ethylene oxide gas in sterilization is 49-63°C (120-145°F).

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Description

Test your knowledge of cold sterilization methods in pharmaceutical microbiology with this quiz. Explore gas sterilization, radiation, and filtration techniques, as well as their applications in sterilizing surgical instruments, medical equipment, and powders.