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Clostridium Perfringens: Toxinotypes and Toxins

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Clostridium Perfringens: Toxinotypes and Toxins

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Questions and Answers

What is the ideal location for collecting specimens of exudates from a wound?

From the surrounding healthy tissue

From the deeper areas of the wound where the infection seems to be most pronounced (correct)

From the drainage site of the wound

From the surface of the wound

What is the characteristic shape of the spore of the bacterium?

Spherical and terminal

Round and bulging

Oval and sub-terminal (correct)

Irregular and non-terminal

What is the primary purpose of Nagler's reaction?

To detect the presence of H2S and NH3

To detect the lecithinase activity (correct)

To determine the anaerobic growth of the bacterium

To identify Gram-positive bacilli

What is the effect of CI.perfringens antiserum on Nagler's reaction?

It inhibits the lecithinase activity Signup and view all the answers

What is the characteristic appearance of colonies on blood agar?

Beta-hemolytic colonies (Double zone of haemolysis) Signup and view all the answers

What is the ideal temperature for anaerobic culture of the bacterium?

37C Signup and view all the answers

What is the characteristic of the bacterium's growth on Robertson's cooked meat medium?

Blackening of meat with the production of H2S and NH3 Signup and view all the answers

What is the biochemical characteristic of CI.perfringens?

It ferments many carbohydrates with acid and gas Signup and view all the answers

What is the result of CI.perfringens on litmus milk?

It acidifies litmus milk with stormy clot production Signup and view all the answers

What is the enzymatic activity of a-toxin (lecithinase) in CI.perfringens?

It hydrolyzes phospholipids in egg-yolk agar Signup and view all the answers