Clostridium Perfringens: Toxinotypes and Toxins

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Questions and Answers

What is the ideal location for collecting specimens of exudates from a wound?

  • From the surrounding healthy tissue
  • From the deeper areas of the wound where the infection seems to be most pronounced (correct)
  • From the drainage site of the wound
  • From the surface of the wound

What is the characteristic shape of the spore of the bacterium?

  • Spherical and terminal
  • Round and bulging
  • Oval and sub-terminal (correct)
  • Irregular and non-terminal

What is the primary purpose of Nagler's reaction?

  • To detect the presence of H2S and NH3
  • To detect the lecithinase activity (correct)
  • To determine the anaerobic growth of the bacterium
  • To identify Gram-positive bacilli

What is the effect of CI.perfringens antiserum on Nagler's reaction?

<p>It inhibits the lecithinase activity (A)</p> Signup and view all the answers

What is the characteristic appearance of colonies on blood agar?

<p>Beta-hemolytic colonies (Double zone of haemolysis) (B)</p> Signup and view all the answers

What is the ideal temperature for anaerobic culture of the bacterium?

<p>37C (B)</p> Signup and view all the answers

What is the characteristic of the bacterium's growth on Robertson's cooked meat medium?

<p>Blackening of meat with the production of H2S and NH3 (B)</p> Signup and view all the answers

What is the biochemical characteristic of CI.perfringens?

<p>It ferments many carbohydrates with acid and gas (A)</p> Signup and view all the answers

What is the result of CI.perfringens on litmus milk?

<p>It acidifies litmus milk with stormy clot production (B)</p> Signup and view all the answers

What is the enzymatic activity of a-toxin (lecithinase) in CI.perfringens?

<p>It hydrolyzes phospholipids in egg-yolk agar (D)</p> Signup and view all the answers

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