Clinical Biochemistry: Calcium, Parathyroid Hormone, Vitamin D Quiz
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Questions and Answers

What is the main effect of vitamin D2 (ergocalciferol) on the body?

  • Increases secretion of parathyroid hormone
  • Increases absorption of calcium in the intestine (correct)
  • Decreases renal excretion of calcium
  • Decreases reabsorption of calcium in the kidneys

Which of the following can interfere with the analytic method for phosphate determination?

  • Oxalate, citrate or EDTA (correct)
  • Lithium heparin plasma
  • 24-hour urine
  • Serum

Which of the following is a potential cause of an elevated anion gap?

  • Hypoalbuminemia
  • Severe hypercalcemia
  • Methanol, ethanol, or ethylene glycol poisoning (correct)
  • Hyponatremia

What is the primary purpose of calculating the anion gap?

<p>To determine the difference between measured and unmeasured anions (A)</p> Signup and view all the answers

What is the primary effect of excessive secretion or administration of growth hormone?

<p>Increases reabsorption of calcium in the kidneys (A)</p> Signup and view all the answers

Which of the following is the most accurate method for determining phosphate levels?

<p>Fiske-Subbarow method with ammonium phosphomolybdate complex (340 nm) (A)</p> Signup and view all the answers

Which of the following is the most important function of the Na*-K*-ATPase pumps?

<p>To regulate the osmotic activity of plasma (D)</p> Signup and view all the answers

What is the primary cause of hyponatremia?

<p>Increased movement of water into the cells, causing a decrease in serum/plasma sodium levels (A)</p> Signup and view all the answers

What is the primary cause of hyperchloremia?

<p>Metabolic alkalosis with a tendency for the plasma chloride level to rise as the bicarbonate level increases (B)</p> Signup and view all the answers

Which method is considered the most accurate for the determination of chloride levels?

<p>Ion-Selective Electrode (B)</p> Signup and view all the answers

What is the primary function of bicarbonate in the extracellular fluid (ECF)?

<p>To serve as the major component of the buffering system in the blood (B)</p> Signup and view all the answers

Which of the following is the most accurate method for the determination of total carbon dioxide (CO2) in plasma?

<p>Ion-Selective Electrode (A)</p> Signup and view all the answers

What is the primary function of magnesium in the body?

<p>To act as an essential cofactor for more than 300 enzymes (D)</p> Signup and view all the answers

Which hormone is primarily responsible for the regulation of magnesium levels in the body?

<p>Parathyroid hormone (C)</p> Signup and view all the answers

What is the recommended specimen for the determination of magnesium levels?

<p>Serum or lithium heparin plasma (B)</p> Signup and view all the answers

Which of the following is the primary cause of an increased anion gap?

<p>Metabolic acidosis caused by increased production or diminished excretion of organic acids (A)</p> Signup and view all the answers

Which hormone is responsible for regulating blood calcium levels?

<p>Parathyroid hormone (A)</p> Signup and view all the answers

What is the typical range for normal plasma osmolality?

<p>275-295 mOsm/kg (A)</p> Signup and view all the answers

Which element is not involved in the blood coagulation process?

<p>Potassium (D)</p> Signup and view all the answers

What is used to calculate the Osmolal Gap?

<p>$2Na + 1.86 Na + glucose (mg/dl)$ (B)</p> Signup and view all the answers

Which cation is the most abundant in the extracellular fluid?

<p>Sodium (D)</p> Signup and view all the answers

Which hormone stimulates the sensation of thirst in response to increased blood osmolality?

<p>Arginine vasopressin (D)</p> Signup and view all the answers

What enzymatic activity does Aspartate Aminotransferase (AST) have?

<p>Transfer of an amino group from alanine to a-ketoglutarate (B)</p> Signup and view all the answers

Which tissue has the highest concentration of Alanine Aminotransferase (ALT)?

<p>Liver (B)</p> Signup and view all the answers

What is the diagnostic significance of Aspartate Aminotransferase (AST) in hepatic disorders?

<p>Increased concentration in acute inflammatory conditions (B)</p> Signup and view all the answers

Which enzymatic activity does Alanine Aminotransferase (ALT) share with Aspartate Aminotransferase (AST)?

<p>Transfer of an amino group from alanine to a-ketoglutarate (C)</p> Signup and view all the answers

What makes Alanine Aminotransferase (ALT) a more sensitive and specific screening test for posttransfusion hepatitis?

<p>More liver-specific than Aspartate Aminotransferase (AST) (D)</p> Signup and view all the answers

Which of the following is the primary organ affected by elevated levels of Aspartate Aminotransferase (AST)?

<p>Liver (C)</p> Signup and view all the answers

What is the primary role of the coupled enzymatic reaction in the measurement of Alanine Aminotransferase (ALT) activity?

<p>To convert alanine and α-ketoglutarate into pyruvate and glutamate (C)</p> Signup and view all the answers

What is the primary function of the 2,4-DNPH reagent in the Reitman and Frankel method for measuring AST and ALT activities?

<p>To act as a color developer for the spectrophotometric analysis (B)</p> Signup and view all the answers

Which of the following is the EARLIEST marker for the diagnosis of acute pancreatitis?

<p>Serum amylase (D)</p> Signup and view all the answers

What is the primary reason for the increased urinary excretion of amylase in acute pancreatitis?

<p>The amylase enzyme is released from the damaged pancreatic acinar cells into the bloodstream and is then filtered by the kidneys. (B)</p> Signup and view all the answers

Which of the following is the most predominant pancreatic amylase isoenzyme in acute pancreatitis?

<p>Isoenzyme P3 (C)</p> Signup and view all the answers

Where is Gamma Glutamyl Transamine Peptidase/Transferase (GGT) primarily located?

<p>Hepatic cells (D)</p> Signup and view all the answers

What is the diagnostic significance of Gamma Glutamyl Transamine Peptidase/Transferase (GGT)?

<p>Sensitive indicator of alcoholism (C)</p> Signup and view all the answers

Which enzyme is secreted by the liver and reflects synthetic function rather than hepatocyte injury?

<p>Pseudocholinesterase (PChE) (C)</p> Signup and view all the answers

What is the primary function of Aspartate Aminotransferase (AST)?

<p>Reflects synthetic function of the liver (A)</p> Signup and view all the answers

What is the diagnostic significance of Alanine Aminotransferase (ALT) among hepatobiliary disorders?

<p>Increased in pancreatitis (A)</p> Signup and view all the answers

What is the primary effect of elevated levels of Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT)?

<p>Implies liver injury or damage (D)</p> Signup and view all the answers

What is the primary characteristic that defines enzymes?

<p>They hasten chemical reactions in organic matter (B)</p> Signup and view all the answers

What happens to the reaction rate when the substrate concentration reaches a maximal value?

<p>The reaction rate remains constant (B)</p> Signup and view all the answers

Which factor affects enzyme reactions by being nonprotein entities that must bind to particular enzymes before a reaction occurs?

<p>Cofactors (D)</p> Signup and view all the answers

In enzyme kinetics, what is the role of coenzymes?

<p>Act as substrates for the enzymes (B)</p> Signup and view all the answers

What is the primary outcome of increasing enzyme concentration in a reaction?

<p>Increased reaction rate due to more substrate binding (C)</p> Signup and view all the answers

Which term describes the situation when substrate concentration surpasses enzyme availability, resulting in a constant reaction rate?

<p><em>Enzyme saturation</em> (C)</p> Signup and view all the answers

What happens when an uncompetitive inhibitor binds to the enzyme-substrate complex?

<p>More ES complexes are formed and inhibition increases (D)</p> Signup and view all the answers

At what temperature is the denaturation of enzymes usually significant?

<p>40°C-50°C (D)</p> Signup and view all the answers

What is the effect of extreme pH levels on enzymes?

<p>Denaturation or structural changes (A)</p> Signup and view all the answers

What does fractionating isoenzymes help in enhancing?

<p>Total enzyme activity (C)</p> Signup and view all the answers

What is the significance of the temperature coefficient (Q10) in enzyme activity?

<p>It increases enzyme activity with temperature rise (A)</p> Signup and view all the answers

In which pH range do most physiological reactions occur?

<p>7-8 (C)</p> Signup and view all the answers

According to the Lock and Key Theory proposed by Emil Fisher, what is the key analogy referring to?

<p>The substrate (A)</p> Signup and view all the answers

Which type of enzymatic reaction follows a rate that is directly proportional to the substrate concentration?

<p>First-order reaction (D)</p> Signup and view all the answers

What is the primary advantage of using a continuous monitoring/kinetic assay method over a fixed-time method for measuring enzyme activity?

<p>It allows for real-time monitoring of the reaction (D)</p> Signup and view all the answers

Which type of enzyme specificity is exhibited when an enzyme reacts with specific chemical bonds?

<p>Bond specificity (C)</p> Signup and view all the answers

According to the Induced Fit Theory proposed by Daniel Koshland, what happens when a substrate binds to an enzyme?

<p>The enzyme's active site changes shape to fit the substrate (B)</p> Signup and view all the answers

Which of the following methods of measuring enzyme activity involves determining the change in coenzyme concentration?

<p>All of the above (D)</p> Signup and view all the answers

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