Clinical Bacteriology and Microbiology Overview

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What is the purpose of Lysine Iron Agar culture media?

Ability to produce lysine decarboxylase enzyme

Why is Triple Sugar Iron Agar used in microbiology?

To differentiate among different groups or genera of the Enterobacteriaceae

What is the recommended action regarding swabs as specimen for microbiological testing?

Swabs are poor specimens

How should specimen containers be prepared for collection?

Use sterile containers Signup and view all the answers

What preservative should be used for urine at room temperature?

Boric acid Signup and view all the answers

What is the ideal time frame for transporting specimens in microbiology?

Within 30 minutes Signup and view all the answers

What specimen storage method is recommended for CSF?

Incubator Signup and view all the answers

Why is it important to collect specimens before administering antibiotics?

To ensure accurate culture results Signup and view all the answers

How should the skin be disinfected before collecting a bone sample?

Before surgery Signup and view all the answers

What is the recommended storage temperature for serum for serological studies?

-20 degrees Celsius Signup and view all the answers

What is the recommended preservative for blood culture?

70% alcohol and iodophore Signup and view all the answers

How long can CSF be stored at 37 degrees Celsius?

6 hours Signup and view all the answers

Who is known as the 'Father of Microbiology'?

Anton van Leeuwenhoek Signup and view all the answers

Who refuted the idea of spontaneous generation by demonstrating that maggots do not spontaneously generate on meat left out in the open air?

Francesco Redi Signup and view all the answers

Who established the first proof that bacteria indeed cause disease and discovered Bacillus anthracis and Mycobacterium tuberculosis?

Robert Koch Signup and view all the answers

What are the steps of Koch's postulates?

<ol> <li>The microorganism must be present in every case of the disease but absent from healthy organisms.</li> <li>Suspected microorganism must be isolated and grown in pure culture.</li> <li>The same disease must result when the isolated microorganism is inoculated in healthy host.</li> <li>The same organism must be isolated again from the diseased host.</li> </ol> Signup and view all the answers

Who experimented on how people can be protected from smallpox and developed attenuated cultures as vaccines?

Louis Pasteur Signup and view all the answers

What is the purpose of the Citrate Utilization Test in microbiology?

To determine the ability of an organism to use citrate as the sole source of energy. Signup and view all the answers

What is the composition of Tryptic Soy Broth culture media?

Pancreatic digest of casein, Sodium chloride, Papaic digest of soybean Meal, Dextrose, Dipotassium Phosphate Signup and view all the answers

Describe the principle behind the Sulfide Indole Motility test.

It detects sulfur production, indole production, and motility of microorganisms. Signup and view all the answers

Who challenged the theory of spontaneous generation with the concept of 'biogenesis'?

Rudolf Virchow Signup and view all the answers

What did Ignaz Semmelweis demonstrate in the field of microbiology?

Routine handwashing can prevent the spread of infection. Signup and view all the answers

What did Joseph Lister develop in the field of surgery?

Antiseptic techniques using phenol. Signup and view all the answers