23 Questions
What is the purpose of Lysine Iron Agar culture media?
Ability to produce lysine decarboxylase enzyme
Why is Triple Sugar Iron Agar used in microbiology?
To differentiate among different groups or genera of the Enterobacteriaceae
What is the recommended action regarding swabs as specimen for microbiological testing?
Swabs are poor specimens
How should specimen containers be prepared for collection?
Use sterile containers
What preservative should be used for urine at room temperature?
Boric acid
What is the ideal time frame for transporting specimens in microbiology?
Within 30 minutes
What specimen storage method is recommended for CSF?
Incubator
Why is it important to collect specimens before administering antibiotics?
To ensure accurate culture results
How should the skin be disinfected before collecting a bone sample?
Before surgery
What is the recommended storage temperature for serum for serological studies?
-20 degrees Celsius
What is the recommended preservative for blood culture?
70% alcohol and iodophore
How long can CSF be stored at 37 degrees Celsius?
6 hours
Who is known as the 'Father of Microbiology'?
Anton van Leeuwenhoek
Who refuted the idea of spontaneous generation by demonstrating that maggots do not spontaneously generate on meat left out in the open air?
Francesco Redi
Who established the first proof that bacteria indeed cause disease and discovered Bacillus anthracis and Mycobacterium tuberculosis?
Robert Koch
What are the steps of Koch's postulates?
- The microorganism must be present in every case of the disease but absent from healthy organisms.
- Suspected microorganism must be isolated and grown in pure culture.
- The same disease must result when the isolated microorganism is inoculated in healthy host.
- The same organism must be isolated again from the diseased host.
Who experimented on how people can be protected from smallpox and developed attenuated cultures as vaccines?
Louis Pasteur
What is the purpose of the Citrate Utilization Test in microbiology?
To determine the ability of an organism to use citrate as the sole source of energy.
What is the composition of Tryptic Soy Broth culture media?
Pancreatic digest of casein, Sodium chloride, Papaic digest of soybean Meal, Dextrose, Dipotassium Phosphate
Describe the principle behind the Sulfide Indole Motility test.
It detects sulfur production, indole production, and motility of microorganisms.
Who challenged the theory of spontaneous generation with the concept of 'biogenesis'?
Rudolf Virchow
What did Ignaz Semmelweis demonstrate in the field of microbiology?
Routine handwashing can prevent the spread of infection.
What did Joseph Lister develop in the field of surgery?
Antiseptic techniques using phenol.
Explore the history and fundamentals of microbiology, including bacteriology, mycology, phycology, and parasitology. Learn about the study of bacteria, fungi, algae, and parasites, their characteristics, and their significance in the field of microbiology.
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