Classification of Microorganisms based on Oxygen Needs

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Where do aerobes grow and what do they use in respiration?

In areas with reduced oxygen levels but they do not use oxygen in respiration.

In full oxygen tension but they do not use oxygen in respiration.

In full oxygen tension and they use oxygen in respiration. (correct)

In areas with reduced oxygen levels and they use oxygen in fermentation.

Which microorganisms are often tolerant of some oxygen but are still considered anaerobic?

Aerotolerant anaerobes (correct)

Microaerophiles

Obligate anaerobes

Facultative anaerobes

What is a characteristic of obligatory aerobes when grown in a thioglycolate broth?

They only grow near the top of the container. (correct)

They do not grow at all in thioglycolate broth.

They only grow at the bottom of the container.

They grow throughout the container.

What technique is commonly used to create an oxygen-free environment for anaerobes?

Using reducing agents like thioglycolate Signup and view all the answers

Which classification of microorganisms can grow both in the presence and absence of oxygen?

Facultative anaerobes Signup and view all the answers

What is a distinguishing feature of methanogens among the archaea group mentioned in the text?

They produce methane and are very sensitive to oxygen. Signup and view all the answers

Which of the following statements accurately describes microaerophiles?

They prefer environments with moderate levels of oxygen, avoiding direct contact with air. Signup and view all the answers

Which technique is NOT mentioned for culturing strict anaerobes?

Supplementation of growth media with reducing agents Signup and view all the answers

Which of the following is an advantage of genotypic classification over phenotypic methods?

Genotypic methods provide a more objective measure of relatedness. Signup and view all the answers

What is the primary advantage of the 16S rRNA gene sequencing method for genotypic classification?

It targets a highly conserved region of the genome that varies between species. Signup and view all the answers

Which of the following statements accurately describes obligate aerobes?

They require molecular oxygen for growth and survival. Signup and view all the answers

Which of the following statements accurately describes anaerobes?

They require special techniques like incubation in oxygen-free gas chambers for culturing. Signup and view all the answers

Which of the following is NOT a criteria used for the classification of prokaryotes within domains?

The ability to form endospores Signup and view all the answers

Which technique is commonly used for culturing obligate aerobes?

Shake flasks or fermenters with continuous aeration Signup and view all the answers

What is a key difference between the culture techniques for aerobes and anaerobes?

Anaerobes require an oxygen-free atmosphere, while aerobes need oxygen Signup and view all the answers

Which of the following is a phenotypic characteristic that can be used for the classification of prokaryotes?

Metabolic pathways utilized for energy production Signup and view all the answers

What is the primary tool mentioned for determining evolutionary relationships and classification of prokaryotes based on genotype?

Analysis of the 16S rRNA gene Signup and view all the answers

Which of the following statements about anaerobes is correct?

Anaerobes can tolerate the presence of oxygen but do not require it Signup and view all the answers