Classification of Microorganisms based on Oxygen Needs
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Questions and Answers

Where do aerobes grow and what do they use in respiration?

  • In areas with reduced oxygen levels but they do not use oxygen in respiration.
  • In full oxygen tension but they do not use oxygen in respiration.
  • In full oxygen tension and they use oxygen in respiration. (correct)
  • In areas with reduced oxygen levels and they use oxygen in fermentation.

Which microorganisms are often tolerant of some oxygen but are still considered anaerobic?

  • Aerotolerant anaerobes (correct)
  • Microaerophiles
  • Obligate anaerobes
  • Facultative anaerobes

What is a characteristic of obligatory aerobes when grown in a thioglycolate broth?

  • They only grow near the top of the container. (correct)
  • They do not grow at all in thioglycolate broth.
  • They only grow at the bottom of the container.
  • They grow throughout the container.

What technique is commonly used to create an oxygen-free environment for anaerobes?

<p>Using reducing agents like thioglycolate (B)</p> Signup and view all the answers

Which classification of microorganisms can grow both in the presence and absence of oxygen?

<p>Facultative anaerobes (A)</p> Signup and view all the answers

What is a distinguishing feature of methanogens among the archaea group mentioned in the text?

<p>They produce methane and are very sensitive to oxygen. (D)</p> Signup and view all the answers

Which of the following statements accurately describes microaerophiles?

<p>They prefer environments with moderate levels of oxygen, avoiding direct contact with air. (A)</p> Signup and view all the answers

Which technique is NOT mentioned for culturing strict anaerobes?

<p>Supplementation of growth media with reducing agents (C)</p> Signup and view all the answers

Which of the following is an advantage of genotypic classification over phenotypic methods?

<p>Genotypic methods provide a more objective measure of relatedness. (A)</p> Signup and view all the answers

What is the primary advantage of the 16S rRNA gene sequencing method for genotypic classification?

<p>It targets a highly conserved region of the genome that varies between species. (B)</p> Signup and view all the answers

Which of the following statements accurately describes obligate aerobes?

<p>They require molecular oxygen for growth and survival. (D)</p> Signup and view all the answers

Which of the following statements accurately describes anaerobes?

<p>They require special techniques like incubation in oxygen-free gas chambers for culturing. (D)</p> Signup and view all the answers

Which of the following is NOT a criteria used for the classification of prokaryotes within domains?

<p>The ability to form endospores (C)</p> Signup and view all the answers

Which technique is commonly used for culturing obligate aerobes?

<p>Shake flasks or fermenters with continuous aeration (A)</p> Signup and view all the answers

What is a key difference between the culture techniques for aerobes and anaerobes?

<p>Anaerobes require an oxygen-free atmosphere, while aerobes need oxygen (D)</p> Signup and view all the answers

Which of the following is a phenotypic characteristic that can be used for the classification of prokaryotes?

<p>Metabolic pathways utilized for energy production (A)</p> Signup and view all the answers

What is the primary tool mentioned for determining evolutionary relationships and classification of prokaryotes based on genotype?

<p>Analysis of the 16S rRNA gene (A)</p> Signup and view all the answers

Which of the following statements about anaerobes is correct?

<p>Anaerobes can tolerate the presence of oxygen but do not require it (A)</p> Signup and view all the answers

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