24 Questions
In which type of chromatography is the stationary phase packed in a closed container?
Adsorption chromatography
Which type of chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase?
Partition chromatography
Which type of chromatography depends on relative solubility of the compound between the sorbent (stationary phase) and the solvent (mobile phase)?
Gel filtration chromatography
Which type of chromatography involves a competition of each component for the active site of the adsorbent?
Adsorption chromatography
What type of chromatography depends on interactions of biological molecules, such as antibody-antigen interaction and DNA-protein interaction?
Affinity chromatography
In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?
Ion-exchange chromatography
How can the partition be turned into adsorption and vice versa?
Drying
Which type of chromatography depends on the relative size of the molecules of the compound being separated?
Gel filtration chromatography
What is the main difference between adsorption chromatography and partition chromatography?
Adsorption chromatography involves the distribution of the solute between a solid stationary phase and a liquid or gas mobile phase, while partition chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase which could be a liquid or a gas.
In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?
Ion-exchange chromatography
What type of chromatography depends on interactions of biological molecules, such as antibody-antigen interaction and DNA-protein interaction?
Affinity chromatography
What type of interactions determine the distribution of solutes in adsorption chromatography?
Weak chemical bonds such as hydrogen bonds, Van der Waal forces, and dipole-dipole interactions
In which type of chromatography is the competition of each component for the active site of the adsorbent a significant factor?
Affinity chromatography
Which type of chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase?
Thin layer chromatography
Which type of chromatography involves the distribution of solutes between a solid stationary phase and a liquid or gas mobile phase?
Adsorption chromatography
How can the partition be turned into adsorption and vice versa in chromatography?
By increasing the temperature
In which type of chromatography does the separation rely on the competition of each component for the active site of the adsorbent?
Affinity chromatography
How can the partition be turned into adsorption and vice versa in chromatography?
By modifying the chemical nature of the stationary phase
In which type of chromatography does the separation rely on differences in affinity between ionic components and the stationary phase?
Ion-exchange chromatography
Which type of chromatography involves a competition of each component for the active site of the adsorbent?
Affinity chromatography
What type of chromatography involves the distribution of solutes between a solid stationary phase and a liquid or gas mobile phase?
Adsorption chromatography
In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?
Ion-exchange chromatography
What is the main difference between gel filtration chromatography and affinity chromatography?
The relative size of the molecules of the compound being separated
Which type of interactions determine the distribution of solutes in adsorption chromatography?
Active sites in stationary and mobile phases (e.g. solutes can form weak chemical bonds: hydrogen bonds, Van der Waal forces, & dipole-dipole interactions)
Test your knowledge on the classification of chromatography with this quiz covering closed column chromatography, open column chromatography, physical arrangement of the system, and different mechanisms of separation.
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