Classification of Chromatography

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Questions and Answers

In which type of chromatography is the stationary phase packed in a closed container?

  • Gel filtration chromatography
  • Ion-exchange chromatography
  • Adsorption chromatography (correct)
  • Partition chromatography

Which type of chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase?

  • Partition chromatography (correct)
  • Gel filtration chromatography
  • Adsorption chromatography
  • Ion-exchange chromatography

Which type of chromatography depends on relative solubility of the compound between the sorbent (stationary phase) and the solvent (mobile phase)?

  • Ion-exchange chromatography
  • Adsorption chromatography
  • Affinity chromatography
  • Gel filtration chromatography (correct)

Which type of chromatography involves a competition of each component for the active site of the adsorbent?

<p>Adsorption chromatography (C)</p> Signup and view all the answers

What type of chromatography depends on interactions of biological molecules, such as antibody-antigen interaction and DNA-protein interaction?

<p>Affinity chromatography (A)</p> Signup and view all the answers

In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?

<p>Ion-exchange chromatography (D)</p> Signup and view all the answers

How can the partition be turned into adsorption and vice versa?

<p>Drying (C)</p> Signup and view all the answers

Which type of chromatography depends on the relative size of the molecules of the compound being separated?

<p>Gel filtration chromatography (C)</p> Signup and view all the answers

What is the main difference between adsorption chromatography and partition chromatography?

<p>Adsorption chromatography involves the distribution of the solute between a solid stationary phase and a liquid or gas mobile phase, while partition chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase which could be a liquid or a gas. (A)</p> Signup and view all the answers

In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?

<p>Ion-exchange chromatography (B)</p> Signup and view all the answers

What type of chromatography depends on interactions of biological molecules, such as antibody-antigen interaction and DNA-protein interaction?

<p>Affinity chromatography (B)</p> Signup and view all the answers

What type of interactions determine the distribution of solutes in adsorption chromatography?

<p>Weak chemical bonds such as hydrogen bonds, Van der Waal forces, and dipole-dipole interactions (C)</p> Signup and view all the answers

In which type of chromatography is the competition of each component for the active site of the adsorbent a significant factor?

<p>Affinity chromatography (A)</p> Signup and view all the answers

Which type of chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase?

<p>Thin layer chromatography (D)</p> Signup and view all the answers

Which type of chromatography involves the distribution of solutes between a solid stationary phase and a liquid or gas mobile phase?

<p>Adsorption chromatography (D)</p> Signup and view all the answers

How can the partition be turned into adsorption and vice versa in chromatography?

<p>By increasing the temperature (B)</p> Signup and view all the answers

In which type of chromatography does the separation rely on the competition of each component for the active site of the adsorbent?

<p>Affinity chromatography (C)</p> Signup and view all the answers

How can the partition be turned into adsorption and vice versa in chromatography?

<p>By modifying the chemical nature of the stationary phase (D)</p> Signup and view all the answers

In which type of chromatography does the separation rely on differences in affinity between ionic components and the stationary phase?

<p>Ion-exchange chromatography (A)</p> Signup and view all the answers

Which type of chromatography involves a competition of each component for the active site of the adsorbent?

<p>Affinity chromatography (C)</p> Signup and view all the answers

What type of chromatography involves the distribution of solutes between a solid stationary phase and a liquid or gas mobile phase?

<p>Adsorption chromatography (B)</p> Signup and view all the answers

In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?

<p>Ion-exchange chromatography (A)</p> Signup and view all the answers

What is the main difference between gel filtration chromatography and affinity chromatography?

<p>The relative size of the molecules of the compound being separated (B)</p> Signup and view all the answers

Which type of interactions determine the distribution of solutes in adsorption chromatography?

<p>Active sites in stationary and mobile phases (e.g. solutes can form weak chemical bonds: hydrogen bonds, Van der Waal forces, &amp; dipole-dipole interactions) (C)</p> Signup and view all the answers

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