Classification of Chromatography
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Questions and Answers

In which type of chromatography is the stationary phase packed in a closed container?

  • Gel filtration chromatography
  • Ion-exchange chromatography
  • Adsorption chromatography (correct)
  • Partition chromatography

    • Which type of chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase?

  • Partition chromatography (correct)
  • Gel filtration chromatography
  • Adsorption chromatography
  • Ion-exchange chromatography

    • Which type of chromatography depends on relative solubility of the compound between the sorbent (stationary phase) and the solvent (mobile phase)?

  • Ion-exchange chromatography
  • Adsorption chromatography
  • Affinity chromatography
  • Gel filtration chromatography (correct)

    • Which type of chromatography involves a competition of each component for the active site of the adsorbent?

    <p>Adsorption chromatography</p> Signup and view all the answers

    What type of chromatography depends on interactions of biological molecules, such as antibody-antigen interaction and DNA-protein interaction?

    <p>Affinity chromatography</p> Signup and view all the answers

    In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?

    <p>Ion-exchange chromatography</p> Signup and view all the answers

    How can the partition be turned into adsorption and vice versa?

    <p>Drying</p> Signup and view all the answers

    Which type of chromatography depends on the relative size of the molecules of the compound being separated?

    <p>Gel filtration chromatography</p> Signup and view all the answers

    What is the main difference between adsorption chromatography and partition chromatography?

    <p>Adsorption chromatography involves the distribution of the solute between a solid stationary phase and a liquid or gas mobile phase, while partition chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase which could be a liquid or a gas.</p> Signup and view all the answers

    In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?

    <p>Ion-exchange chromatography</p> Signup and view all the answers

    What type of chromatography depends on interactions of biological molecules, such as antibody-antigen interaction and DNA-protein interaction?

    <p>Affinity chromatography</p> Signup and view all the answers

    What type of interactions determine the distribution of solutes in adsorption chromatography?

    <p>Weak chemical bonds such as hydrogen bonds, Van der Waal forces, and dipole-dipole interactions</p> Signup and view all the answers

    In which type of chromatography is the competition of each component for the active site of the adsorbent a significant factor?

    <p>Affinity chromatography</p> Signup and view all the answers

    Which type of chromatography involves the distribution of the solute between a liquid stationary phase and a mobile phase?

    <p>Thin layer chromatography</p> Signup and view all the answers

    Which type of chromatography involves the distribution of solutes between a solid stationary phase and a liquid or gas mobile phase?

    <p>Adsorption chromatography</p> Signup and view all the answers

    How can the partition be turned into adsorption and vice versa in chromatography?

    <p>By increasing the temperature</p> Signup and view all the answers

    In which type of chromatography does the separation rely on the competition of each component for the active site of the adsorbent?

    <p>Affinity chromatography</p> Signup and view all the answers

    How can the partition be turned into adsorption and vice versa in chromatography?

    <p>By modifying the chemical nature of the stationary phase</p> Signup and view all the answers

    In which type of chromatography does the separation rely on differences in affinity between ionic components and the stationary phase?

    <p>Ion-exchange chromatography</p> Signup and view all the answers

    Which type of chromatography involves a competition of each component for the active site of the adsorbent?

    <p>Affinity chromatography</p> Signup and view all the answers

    What type of chromatography involves the distribution of solutes between a solid stationary phase and a liquid or gas mobile phase?

    <p>Adsorption chromatography</p> Signup and view all the answers

    In which type of chromatography is the separation achieved by differences in affinity between ionic components and the stationary phase?

    <p>Ion-exchange chromatography</p> Signup and view all the answers

    What is the main difference between gel filtration chromatography and affinity chromatography?

    <p>The relative size of the molecules of the compound being separated</p> Signup and view all the answers

    Which type of interactions determine the distribution of solutes in adsorption chromatography?

    <p>Active sites in stationary and mobile phases (e.g. solutes can form weak chemical bonds: hydrogen bonds, Van der Waal forces, &amp; dipole-dipole interactions)</p> Signup and view all the answers

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