Citric Acid Cycle: Energy Metabolism

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Questions and Answers

What form must fuel molecules take to enter the citric acid cycle?

  • A fatty acid
  • Acetyl coenzyme A (correct)
  • A dicarboxylic acid
  • An amino acid

In the context of the citric acid cycle, what is the primary fate of acetate?

  • Reduction to ethanol
  • Conversion to pyruvate
  • Incorporation into fatty acids
  • Oxidation to $CO_2$ (correct)

Which of the following is the primary role of the citric acid cycle in aerobic metabolism?

  • To directly produce large amounts of ATP
  • To oxidize fuel molecules and produce reduced electron carriers. (correct)
  • To synthesize amino acids and nucleotides
  • To regenerate NADP+ for glycolysis.

Which of the following is a direct output of each turn of the citric acid cycle?

<p>One molecule of GTP (A)</p> Signup and view all the answers

What is the ultimate destination of NADH and $FADH_2$ produced during the citric acid cycle?

<p>Oxidative phosphorylation (C)</p> Signup and view all the answers

Besides energy production, what other crucial role does the citric acid cycle play in the cell?

<p>Supply of precursors for anabolic processes (B)</p> Signup and view all the answers

Where within the cell do all the enzymatic reactions of the citric acid cycle take place?

<p>Mitochondrial matrix or inner mitochondrial membrane (A)</p> Signup and view all the answers

How many molecules of $CO_2$ are produced per molecule of acetyl-CoA that enters the citric acid cycle?

<p>2 (D)</p> Signup and view all the answers

What is the net production of NADH from one turn of the citric acid cycle?

<p>Three (A)</p> Signup and view all the answers

Which of the following reactions in the citric acid cycle involves the direct consumption of water?

<p>Conversion of fumarate to malate (D)</p> Signup and view all the answers

Which enzyme catalyzes the condensation of acetyl-CoA with oxaloacetate to form citrate?

<p>Citrate synthase (C)</p> Signup and view all the answers

What is the role of the enzyme aconitase in the citric acid cycle?

<p>To convert citrate to isocitrate via cis-aconitate (B)</p> Signup and view all the answers

Which enzyme catalyzes the first oxidation reaction in the citric acid cycle?

<p>Isocitrate dehydrogenase (A)</p> Signup and view all the answers

Which of the following enzymatic reactions in the citric acid cycle is similar to the reaction converting pyruvate to acetyl-CoA?

<p>Conversion of $\alpha$-ketoglutarate to succinyl-CoA (A)</p> Signup and view all the answers

The conversion of succinyl-CoA to succinate is coupled to which of the following?

<p>GTP synthesis (A)</p> Signup and view all the answers

Which enzyme catalyzes the oxidation of succinate to fumarate in the citric acid cycle?

<p>Succinate dehydrogenase (B)</p> Signup and view all the answers

What type of reaction is catalyzed by fumarase in the citric acid cycle?

<p>Hydration (A)</p> Signup and view all the answers

Which enzyme catalyzes the final oxidation reaction in the citric acid cycle, regenerating oxaloacetate?

<p>Malate dehydrogenase (D)</p> Signup and view all the answers

Which of the following best describes the regulation of pyruvate dehydrogenase?

<p>Inhibited by ATP, acetyl-CoA, and NADH (A)</p> Signup and view all the answers

What limits the activity of citrate synthase?

<p>Availability of oxaloacetate (B)</p> Signup and view all the answers

How is isocitrate dehydrogenase regulated?

<p>Activated by ADP, inhibited by NADH (A)</p> Signup and view all the answers

By what mechanism is $\alpha$-ketoglutarate dehydrogenase regulated?

<p>Inhibited allosterically by products (D)</p> Signup and view all the answers

Which of the following describes a key function of the pentose phosphate pathway?

<p>To provide NADPH and ribose 5-phosphate for biosynthesis (D)</p> Signup and view all the answers

Which electron carrier is primarily used to reduce substrates and functions synthetically, such as in steroid and fatty acid synthesis?

<p>NADP+ (C)</p> Signup and view all the answers

Where does the pentose phosphate pathway take place within the cell?

<p>Cytosol (D)</p> Signup and view all the answers

What are the two main phases of the pentose phosphate pathway?

<p>Oxidative and nonoxidative phases (A)</p> Signup and view all the answers

Which of the following is directly generated in the oxidative phase of the pentose phosphate pathway?

<p>NADPH (B)</p> Signup and view all the answers

The first committed step in the oxidative phase of the pentose phosphate pathway is catalyzed by which enzyme?

<p>Glucose-6-phosphate dehydrogenase (C)</p> Signup and view all the answers

What type of reaction is catalyzed by 6-phosphogluconate dehydrogenase in the pentose phosphate pathway?

<p>Decarboxylation (D)</p> Signup and view all the answers

What is the function of ribose phosphate isomerase in the pentose phosphate pathway?

<p>To convert ribulose 5-phosphate to ribose 5-phosphate (D)</p> Signup and view all the answers

What is the fate of ribulose 5-phosphate in cells that require more NADPH than pentose phosphates?

<p>It is converted into glucose precursors in the nonoxidative phase. (B)</p> Signup and view all the answers

Which of the following conversions occurs during the nonoxidative phase of the pentose phosphate pathway?

<p>Ribulose 5-phosphate to glyceraldehyde 3-phosphate and fructose 6-phosphate (B)</p> Signup and view all the answers

Flashcards

Citric Acid Cycle

A series of chemical reactions that extract energy from acetyl-CoA and produce electron carriers (NADH, FADH2) and GTP.

Citric Acid Cycle's Role

The final common pathway for the oxidation of fuel molecules, including amino acids, fatty acids, and carbohydrates.

Acetyl Coenzyme A

Fuel molecules enter the cycle in this form.

Products per cycle

One molecule of GTP; three molecules of NADH; one molecule of FADH2.

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First step of TCA cycle

Oxaloacetate condenses with acetyl-CoA to form citrate.

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Second step of TCA cycle

Citrate is converted to isocitrate via cis-aconitate (enzyme: aconitase).

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Third step of TCA cycle

isocitrate is oxidized to α-ketoglutarate, producing CO2 and NADH (enzyme: isocitrate dehydrogenase).

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Fourth step of TCA cycle

α-ketoglutarate is oxidized to succinyl-CoA, producing CO2 and NADH (enzyme: α-ketoglutarate dehydrogenase complex).

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Fifth step of TCA cycle

Succinyl-CoA is converted to succinate, producing GTP (enzyme: succinyl CoA synthetase).

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Sixth step of TCA cycle

Succinate is oxidized to fumarate, producing FADH2 (enzyme: succinate dehydrogenase).

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Seventh step of TCA cycle

Fumarate is hydrated to form L-malate (enzyme: fumarase).

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Eighth step of TCA cycle

Malate is oxidized to oxaloacetate, producing NADH (enzyme: malate dehydrogenase).

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Pentose Phosphate Shunt

Oxidation of glucose to provide NADPH and ribose 5-phosphate for biosynthesis.

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Functions of PPP

Generates NADPH, pentose phosphates, and interconverts C3-C7 carbohydrates.

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Subcellular Location of PPP

Enzymes are soluble in the cytosol.

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Two Phases of PPP

Oxidative and non-oxidative phases.

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Oxidative Phase of PPP

Generates NADPH from glucose-6-phosphate.

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Isomerization in PPP

converts ribulose 5-phosphate to ribose 5-phosphate

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Overall Reaction of First Phase (PPP)

Glucose-6-phosphate + 2 NADP+ + H2O -> Ribose-5-phosphate + CO2 + 2 NADPH + 2 H+

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Nonoxidative Phase of PPP

Three pentose phosphates are transformed into two hexose phosphates and one triose phosphate.

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