Chrome Extension Best Practices Quiz: Test your knowledge of recommended practic...
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Chrome Extension Best Practices Quiz: Test your knowledge of recommended practic...

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Questions and Answers

What is the consensus sequence for the -10 element of the promoter in prokaryotic DNA?

  • TATAAT/A (correct)
  • TTGATC
  • TTGACA
  • Pribno box

    • What is the function of an operator in gene expression regulation?

  • Promoter regions where activators (of transcription) bind
  • Stimulate RNA Pol binding to the promoter
  • Promoter regions where repressors (of transcription) bind (correct)
  • Inhibit RNA Pol binding to the promoter

    • What is the definition of polycistronic mRNA?

  • A transcript that encodes only one protein
  • A transcript that encodes a single gene
  • A transcript that encodes several proteins (correct)
  • A transcript that encodes a regulatory DNA-binding protein

    • What is the function of an operon?

    <p>An expression unit consisting of co-transcribed genes and the operator and promoter sequences</p> Signup and view all the answers

    What is the role of a coactivator in transcriptional regulation?

    <p>Assist activators in enhancing transcription</p> Signup and view all the answers

    What is the primary function of the -35 element in the promoter of prokaryotic DNA?

    <p>It usually consists of the six nucleotides TTGACA</p> Signup and view all the answers

    What does a corepressor do in gene expression regulation?

    <p>Assist repressors in inhibiting transcription</p> Signup and view all the answers

    What is the function of an insulator in gene expression regulation?

    <p>Block the interaction between enhancers and promoters</p> Signup and view all the answers

    What is the characteristic of prokaryotic genes in terms of expression?

    <p>Mostly constitutively expressed</p> Signup and view all the answers

    What is the role of an effector in transcriptional regulation?

    <p>Modulate the activity of regulatory proteins</p> Signup and view all the answers

    What is the function of a core promoter in gene expression?

    <p>It is the site where the basal transcription machinery assembles</p> Signup and view all the answers

    What is the definition of a regulon?

    <p>A set of operons controlled by the same regulatory protein</p> Signup and view all the answers

    Which type of regulation involves repressor proteins inhibiting transcription by binding to the promoter?

    <p>Negative regulation</p> Signup and view all the answers

    What is the function of insulators in gene expression regulation?

    <p>Block unintended effects of DNA looping and control gene expression</p> Signup and view all the answers

    What is the role of effectors in gene expression regulation?

    <p>Small molecules that can bind activators or repressors, causing conformational changes that increase or decrease transcription</p> Signup and view all the answers

    What is the function of a regulon in molecular biology?

    <p>Refers to a group of genes or operons that are coordinately regulated, whether spatially distant or not</p> Signup and view all the answers

    What is the primary role of the bacterial Lac repressor?

    <p>Binds distant DNA sites to form a DNA loop</p> Signup and view all the answers

    What can inactivate a repressor, enabling transcription?

    <p>Effectors</p> Signup and view all the answers

    What facilitates the coordinated transcription of several genes in the SOS system in response to DNA damage?

    <p>RecA protein and LexA repressor protein</p> Signup and view all the answers

    What induces autocleavage of the LexA repressor in the SOS system?

    <p>DNA damage</p> Signup and view all the answers

    What is the role of RecA protein in the SOS system?

    <p>Binds sites of single-stranded DNA and facilitates the destruction of LexA</p> Signup and view all the answers

    What is the function of DNA looping in gene expression regulation?

    <p>Brings distant regulatory sites together to enable transcription</p> Signup and view all the answers

    What is the function of global regulation of gene expression?

    <p>Involves coordinated expression of several genes in response to stress</p> Signup and view all the answers

    What is the role of insulators in prokaryotes and eukaryotes?

    <p>Block unintended effects of DNA looping and control gene expression</p> Signup and view all the answers

    Study Notes

    Regulation of Gene Expression in Molecular Biology

    • Regulon refers to a group of genes or operons that are coordinately regulated, whether spatially distant or not.
    • Transcriptional regulation can be negative, where repressor proteins inhibit transcription by binding to the promoter, or positive, where activator proteins recruit RNA polymerase to initiate transcription.
    • DNA looping, facilitated by transcription activators and architectural regulators, can bring distant regulatory sites together to enable transcription.
    • The bacterial Lac repressor, made of four subunits, binds distant DNA sites to form a DNA loop.
    • Insulators, present in prokaryotes and eukaryotes, block unintended effects of DNA looping and control gene expression.
    • Effectors, small molecules, can bind activators or repressors, causing conformational changes that increase or decrease transcription.
    • Effectors can inactivate a repressor, enabling transcription, or activate a repressor, shutting down transcription.
    • Effectors can also inactivate or enable activators to stimulate transcription.
    • Global regulation of gene expression involves coordinated expression of several genes in response to stress, occurring in prokaryotes and eukaryotes.
    • Coordinated transcription of several genes in the SOS system involves the RecA protein and LexA repressor protein in response to DNA damage.
    • In the SOS system, DNA damage induces autocleavage of LexA repressor, facilitated by RecA protein, leading to the induction of the SOS response.
    • DNA damage creates sites of single-stranded DNA, which are bound by RecA protein, and their association facilitates the destruction of LexA and induction of the SOS response.

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    Related Documents

    Lecture 11 Tr Reg Gene Exp-I (1).pdf

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    Chrome Extension Best Practices Quiz: Test your knowledge of recommended practices for Chrome extensions with this interactive quiz. Learn and understand the key concepts for developing efficient and user-friendly Chrome extensions. Assess your expertise and improve your skills in extension development.

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