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Chromatography Terms and Concepts
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Chromatography Terms and Concepts

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Questions and Answers

In linear chromatography, what type of peak is observed as a result of the separation?

  • Exponential peak
  • Rectangular peak
  • Triangular peak
  • Gaussian peak (correct)
  • What is the equation for the distribution constant (K) in chromatography?

  • K = CS - CM
  • K = CM/CS
  • K = CS * CM
  • K = CS/CM (correct)
  • What is the equation for the average linear velocity of solute (V')?

  • V' = L / tR (correct)
  • V' = tR / L
  • V' = L * tR
  • V' = L - tR
  • What does tM represent in chromatography?

    <p>Retention time of the mobile phase</p> Signup and view all the answers

    What does U represent in the context of linear velocity of the mobile phase?

    <p>Linear velocity of the mobile phase</p> Signup and view all the answers

    What does the distribution constant (K) in chromatography represent?

    <p>The ratio of solute concentration in the stationary phase to the mobile phase</p> Signup and view all the answers

    What does the average linear velocity of solute (V') represent in chromatography?

    <p>The speed at which the solute travels through the column</p> Signup and view all the answers

    What is the retention time of an un-retained substance in chromatography denoted by tM?

    <p>The time spent by the mobile phase in the column (dead time)</p> Signup and view all the answers

    What is indicated by a linear chromatography separation obeying equation (1)?

    <p>The separation follows a distribution constant ratio of solute concentration in both phases</p> Signup and view all the answers

    What does tR' denote in chromatography?

    <p>The time spent by the analyte in the stationary phase (adjusted retention time)</p> Signup and view all the answers

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