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Chromatography Terms and Concepts

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Questions and Answers

In linear chromatography, what type of peak is observed as a result of the separation?

Exponential peak
Rectangular peak
Triangular peak
Gaussian peak (correct)

What is the equation for the distribution constant (K) in chromatography?

K = CS - CM
K = CM/CS
K = CS * CM
K = CS/CM (correct)

What is the equation for the average linear velocity of solute (V')?

V' = L / tR (correct)
V' = tR / L
V' = L * tR
V' = L - tR

What does tM represent in chromatography?

Retention time of the mobile phase (D) Signup and view all the answers

What does U represent in the context of linear velocity of the mobile phase?

Linear velocity of the mobile phase (A) Signup and view all the answers

What does the distribution constant (K) in chromatography represent?

The ratio of solute concentration in the stationary phase to the mobile phase (C) Signup and view all the answers

What does the average linear velocity of solute (V') represent in chromatography?

The speed at which the solute travels through the column (D) Signup and view all the answers

What is the retention time of an un-retained substance in chromatography denoted by tM?

The time spent by the mobile phase in the column (dead time) (D) Signup and view all the answers

What is indicated by a linear chromatography separation obeying equation (1)?

The separation follows a distribution constant ratio of solute concentration in both phases (C) Signup and view all the answers

What does tR' denote in chromatography?

The time spent by the analyte in the stationary phase (adjusted retention time) (C) Signup and view all the answers